{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T20:01:15Z","timestamp":1772740875542,"version":"3.50.1"},"reference-count":44,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,4]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n                  <jats:p>\n                    Filter paper blood samples (i.e., dry blood spots [DBSs]) have been widely used for pathogen detection, especially in malaria control and elimination. Dried blood spot-based polymerase chain reaction (PCR) testing has also been increasingly used for diagnosis and gene monitoring. The current method of DNA extraction from DBSs is limited in practical application because of its low efficiency, time-consuming procedure, and high cost. Accordingly, a hemolysis preservation (HP) method was developed and compared with the most commonly used Tween-Chelex method and spin-column method kit (QIAamp DNA Mini) under equivalent conditions by extracting template DNA from the same batch of\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    density-gradient DBSs. The HP method yielded the highest DNA recovery volume (557.32 ng\/\n                    <jats:italic>\u00b5<\/jats:italic>\n                    L of blood) and the lowest limit of detection (LOD; equivalent to 0.1 parasites\/\n                    <jats:italic>\u00b5<\/jats:italic>\n                    L) from DBSs with the minimum blood volume (4\u20135\n                    <jats:italic>\u00b5<\/jats:italic>\n                    L) using the simplest reagent. Additionally, it had the shortest time (\u223c50 minutes) and the lowest cost ($0.27 per sample). The LOD of the direct PCR method also reached a value of 0.11 parasites\/\n                    <jats:italic>\u00b5<\/jats:italic>\n                    L. Therefore, the DBS PCR test conducted using the HP method can detect asymptomatic low-density\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    infection and is a cost-effective option in resource-deficient areas.\n                  <\/jats:p>","DOI":"10.4269\/ajtmh.25-0115","type":"journal-article","created":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T16:49:18Z","timestamp":1768495758000},"page":"578-583","source":"Crossref","is-referenced-by-count":0,"title":["A Novel Method for the Rapid and Efficient Preparation of Polymerase Chain Reaction Template DNA from Filter Paper Blood Samples"],"prefix":"10.4269","volume":"114","author":[{"given":"Huang","family":"Yuting","sequence":"first","affiliation":[{"name":"Guizhou Provincial Center for Disease Control and Prevention, Guiyang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hu","family":"Jiahuan","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Center for Disease Control and Prevention, Guiyang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Lidan","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Center for Disease Control and Prevention, Guiyang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"She","family":"Danya","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Center for Disease Control and Prevention, Guiyang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huang","family":"Tianyi","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Center for Disease Control and Prevention, Guiyang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Am J Trop Med Hyg","first-page":"256","article-title":"Dried blood spots for global health diagnostics and surveillance: Opportunities and challenges","volume":"99","author":"Lim","year":"2018"},{"key":"b2","series-title":"J Vector Borne Dis","first-page":"11","article-title":"Dried blood spots: A robust tool for malaria surveillance in countries targeting elimination","volume":"60","author":"Sharma","year":"2023"},{"key":"b3","series-title":"Am J Trop Med Hyg","first-page":"922","article-title":"The effect of storage and extraction methods on amplification of Plasmodium falciparum DNA from dried blood spots","volume":"92","author":"Schwartz","year":"2015"},{"key":"b4","series-title":"Rev Inst Med Trop S Paulo","first-page":"205","article-title":"Evaluation of three different DNA extraction methods from blood samples collected in dried filter paper in Plasmodium subpatent infections from the Amazon region in Brazil","volume":"55","author":"Miguel","year":"2013"},{"key":"b5","series-title":"Trans R Soc Trop Med Hyg","first-page":"488","article-title":"Comparison of four methods for extracting DNA from dried blood on filter paper for PCR targeting the mitochondrial Plasmodium genome","volume":"108","author":"Str\u00f8m","year":"2014"},{"key":"b6","series-title":"Malar J","first-page":"50","article-title":"Molecular methods for tracking residual Plasmodium falciparum transmission in a close-to-elimination setting in Zanzibar","volume":"19","author":"Grossenbacher","year":"2020"},{"key":"b7","series-title":"Malar J","first-page":"116","article-title":"Optimization of whole-genome sequencing of Plasmodium falciparum from low-density dried blood spot samples","volume":"20","author":"Teyssier","year":"2021"},{"key":"b8","series-title":"Malar J","first-page":"377","article-title":"A novel method for extracting nucleic acids from dried blood spots for ultrasensitive detection of low-density Plasmodium falciparum and Plasmodium vivax infection","volume":"16","author":"Zainabadi","year":"2017"},{"key":"b9","series-title":"Malar J","first-page":"266","article-title":"Filter paper collection of Plasmodium falciparum mRNA for detecting low-density gametocytes","volume":"11","author":"Jones","year":"2012"},{"key":"b10","series-title":"J Trop Med","first-page":"5715436","article-title":"The comparison of PCR kits for the detection of erythrocytic parasites on filter paper","volume":"2022","author":"Tao","year":"2022"},{"key":"b11","series-title":"Biotech Bull","first-page":"221","article-title":"Study of FTA cards and general qualitative filter paper method for extracting DNA","volume":"11","author":"Fei","year":"2011"},{"key":"b12","series-title":"Malar J","first-page":"192","article-title":"An improved nucleic acid extraction method from dried blood spots for amplification of Plasmodium falciparum kelch13 for detection of artemisinin resistance","volume":"18","author":"Zainabadi","year":"2019"},{"key":"b13","series-title":"Sci Rep","first-page":"4845","article-title":"Comparative performance of PCR using DNA extracted from dried blood spots and whole blood samples for malaria diagnosis: A meta-analysis","volume":"11","author":"Mahittikorn","year":"2021"},{"key":"b14","series-title":"Malar J","first-page":"244","article-title":"Real-time PCR detection of Plasmodium directly from whole blood and filter paper samples","volume":"10","author":"Taylor","year":"2011"},{"key":"b15","series-title":"Malar J","first-page":"137","article-title":"PCR targeting Plasmodium mitochondrial genome of DNA extracted from dried blood on filter paper compared to whole blood","volume":"13","author":"Str\u00f8m","year":"2014"},{"key":"b16","series-title":"Am J Trop Med Hyg","first-page":"573","article-title":"Malaria PCR detection in Cambodian low-transmission settings: Dried blood spots versus venous blood samples","volume":"92","author":"Canier","year":"2015"},{"key":"b17","series-title":"Malar J","first-page":"88","article-title":"Tenfold difference in DNA recovery rate: Systematic comparison of whole blood vs. dried blood spot sample collection for malaria molecular surveillance","volume":"21","author":"Holzschuh","year":"2022"},{"key":"b18","series-title":"J Clin Microbiol","first-page":"1628","article-title":"Novel nested direct PCR technique for malaria diagnosis using filter paper samples","volume":"49","author":"Fuehrer","year":"2011"},{"key":"b19","series-title":"J Infect Dis","first-page":"1322","article-title":"Numerical distributions of parasite densities during asymptomatic malaria","volume":"213","author":"Imwong","year":"2016"},{"key":"b20","series-title":"J Parasit Dis","first-page":"337","article-title":"Comparison between different methods of DNA isolation from dried blood spots for determination of malaria to determine specificity and cost effectiveness","volume":"43","author":"Panda","year":"2019"},{"key":"b21","series-title":"Am J Trop Med Hyg","first-page":"249","article-title":"Short report: Rapid DNA extraction from archive blood spots on filter paper for genotyping of Plasmodium falciparum.","volume":"72","author":"Bereczky","year":"2005"},{"key":"b22","series-title":"Mol Biochem Parasitol","first-page":"111557","article-title":"A simple alkali lysis method for Plasmodium falciparum DNA extraction from filter paper blood samples","volume":"254","author":"Xiang","year":"2023"},{"key":"b1","series-title":"Am J Trop Med Hyg","first-page":"256","article-title":"Dried blood spots for global health diagnostics and surveillance: Opportunities and challenges","volume":"99","author":"Lim","year":"2018"},{"key":"b2","series-title":"J Vector Borne Dis","first-page":"11","article-title":"Dried blood spots: A robust tool for malaria surveillance in countries targeting elimination","volume":"60","author":"Sharma","year":"2023"},{"key":"b3","series-title":"Am J Trop Med Hyg","first-page":"922","article-title":"The effect of storage and extraction methods on amplification of Plasmodium falciparum DNA from dried blood spots","volume":"92","author":"Schwartz","year":"2015"},{"key":"b4","series-title":"Rev Inst Med Trop S Paulo","first-page":"205","article-title":"Evaluation of three different DNA extraction methods from blood samples collected in dried filter paper in Plasmodium subpatent infections from the Amazon region in Brazil","volume":"55","author":"Miguel","year":"2013"},{"key":"b5","series-title":"Trans R Soc Trop Med Hyg","first-page":"488","article-title":"Comparison of four methods for extracting DNA from dried blood on filter paper for PCR targeting the mitochondrial Plasmodium genome","volume":"108","author":"Str\u00f8m","year":"2014"},{"key":"b6","series-title":"Malar J","first-page":"50","article-title":"Molecular methods for tracking residual Plasmodium falciparum transmission in a close-to-elimination setting in Zanzibar","volume":"19","author":"Grossenbacher","year":"2020"},{"key":"b7","series-title":"Malar J","first-page":"116","article-title":"Optimization of whole-genome sequencing of Plasmodium falciparum from low-density dried blood spot samples","volume":"20","author":"Teyssier","year":"2021"},{"key":"b8","series-title":"Malar J","first-page":"377","article-title":"A novel method for extracting nucleic acids from dried blood spots for ultrasensitive detection of low-density Plasmodium falciparum and Plasmodium vivax infection","volume":"16","author":"Zainabadi","year":"2017"},{"key":"b9","series-title":"Malar J","first-page":"266","article-title":"Filter paper collection of Plasmodium falciparum mRNA for detecting low-density gametocytes","volume":"11","author":"Jones","year":"2012"},{"key":"b10","series-title":"J Trop Med","first-page":"5715436","article-title":"The comparison of PCR kits for the detection of erythrocytic parasites on filter paper","volume":"2022","author":"Tao","year":"2022"},{"key":"b11","series-title":"Biotech Bull","first-page":"221","article-title":"Study of FTA cards and general qualitative filter paper method for extracting DNA","volume":"11","author":"Fei","year":"2011"},{"key":"b12","series-title":"Malar J","first-page":"192","article-title":"An improved nucleic acid extraction method from dried blood spots for amplification of Plasmodium falciparum kelch13 for detection of artemisinin resistance","volume":"18","author":"Zainabadi","year":"2019"},{"key":"b13","series-title":"Sci Rep","first-page":"4845","article-title":"Comparative performance of PCR using DNA extracted from dried blood spots and whole blood samples for malaria diagnosis: A meta-analysis","volume":"11","author":"Mahittikorn","year":"2021"},{"key":"b14","series-title":"Malar J","first-page":"244","article-title":"Real-time PCR detection of Plasmodium directly from whole blood and filter paper samples","volume":"10","author":"Taylor","year":"2011"},{"key":"b15","series-title":"Malar J","first-page":"137","article-title":"PCR targeting Plasmodium mitochondrial genome of DNA extracted from dried blood on filter paper compared to whole blood","volume":"13","author":"Str\u00f8m","year":"2014"},{"key":"b16","series-title":"Am J Trop Med Hyg","first-page":"573","article-title":"Malaria PCR detection in Cambodian low-transmission settings: Dried blood spots versus venous blood samples","volume":"92","author":"Canier","year":"2015"},{"key":"b17","series-title":"Malar J","first-page":"88","article-title":"Tenfold difference in DNA recovery rate: Systematic comparison of whole blood vs. dried blood spot sample collection for malaria molecular surveillance","volume":"21","author":"Holzschuh","year":"2022"},{"key":"b18","series-title":"J Clin Microbiol","first-page":"1628","article-title":"Novel nested direct PCR technique for malaria diagnosis using filter paper samples","volume":"49","author":"Fuehrer","year":"2011"},{"key":"b19","series-title":"J Infect Dis","first-page":"1322","article-title":"Numerical distributions of parasite densities during asymptomatic malaria","volume":"213","author":"Imwong","year":"2016"},{"key":"b20","series-title":"J Parasit Dis","first-page":"337","article-title":"Comparison between different methods of DNA isolation from dried blood spots for determination of malaria to determine specificity and cost effectiveness","volume":"43","author":"Panda","year":"2019"},{"key":"b21","series-title":"Am J Trop Med Hyg","first-page":"249","article-title":"Short report: Rapid DNA extraction from archive blood spots on filter paper for genotyping of Plasmodium falciparum.","volume":"72","author":"Bereczky","year":"2005"},{"key":"b22","series-title":"Mol Biochem Parasitol","first-page":"111557","article-title":"A simple alkali lysis method for Plasmodium falciparum DNA extraction from filter paper blood samples","volume":"254","author":"Xiang","year":"2023"}],"container-title":["The American Journal of Tropical Medicine and 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