{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T10:31:19Z","timestamp":1783506679725,"version":"3.55.0"},"reference-count":64,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","license":[{"start":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T00:00:00Z","timestamp":1764720000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,12,3]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n                  <jats:p>\n                    The coronavirus disease 2019 pandemic showcased the power of genomic surveillance in tracking infectious diseases, driving rapid public health responses, and global collaboration. This same infrastructure is being leveraged for malaria molecular surveillance (MMS) in Africa to address challenges such as artemisinin partial resistance and deletions in the\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    histidine-rich protein 2 and 3 genes. However, variability in reporting sequencing methods and data reporting currently limits the validation, comparability, and reuse of data. To maximize the impact of MMS, minimal and optimal data that are key for validation and maximizing transparency and findable, accessible, interoperable, and reusable principles are proposed for reporting. Rather than focusing on specific data formats, in the current study, the authors propose what should be reported and why. Progressing to reporting individual infection-level polymorphism or microhaplotype data is central to maximizing the impact of MMS. Reporting must adhere to local regulatory practices and ensure proper data oversight and management, preventing data colonialism and preserving opportunities for data generators. With malaria\u2019s challenges transcending borders, reporting and adopting standardized practices are essential to advancing research and strengthening global public health efforts.\n                  <\/jats:p>","DOI":"10.4269\/ajtmh.25-0147","type":"journal-article","created":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T18:07:19Z","timestamp":1758823639000},"page":"1169-1177","source":"Crossref","is-referenced-by-count":1,"title":["Toward Setting Minimum and Optimal Data to Report for Malaria Molecular Surveillance with Targeted Sequencing: The \u201cWhat\u201d and \u201cWhy\u201d"],"prefix":"10.4269","volume":"113","author":[{"given":"Jonathan J.","family":"Juliano","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, School of Medicine, University of North Carolina, Chapel Hill, North Carolina;"},{"name":"Institute for Global Health and Infectious Diseases, University of North Carolina, Chapel Hill, North Carolina;"},{"name":"Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina;"},{"name":"Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecile P. 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School of Medicine, University of North Carolina, Chapel Hill, North Carolina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan B.","family":"Parr","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, School of Medicine, University of North Carolina, Chapel Hill, North Carolina;"},{"name":"Institute for Global Health and Infectious Diseases, University of North Carolina, Chapel Hill, North Carolina;"},{"name":"Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa D.","family":"Conrad","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California- San Francisco, San Francisco, California;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob M.","family":"Sadler","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, School of Medicine, University of North Carolina, Chapel Hill, North Carolina;"},{"name":"Institute for Global Health and Infectious Diseases, University of North Carolina, Chapel Hill, North Carolina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher M.","family":"Hennelly","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, School of Medicine, University of North Carolina, Chapel Hill, North Carolina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashenafi","family":"Assefa","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, School of Medicine, University of North Carolina, Chapel Hill, North Carolina;"},{"name":"Ethiopian Public Health Institute, Addis Ababa, 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COVID-19 testing laboratory in resource-limited settings: Challenges and prospects reported from Ethiopia","volume":"13","author":"Abera","year":"2020"},{"key":"b3","series-title":"Nat Commun","first-page":"5006","article-title":"Inference of person-to-person transmission of COVID-19 reveals hidden super-spreading events during the early outbreak phase","volume":"11","author":"Wang","year":"2020"},{"key":"b4","series-title":"JAMA Netw Open","first-page":"e2024191","article-title":"Analysis of genomic characteristics and transmission routes of patients with confirmed SARS-CoV-2 in Southern California during the early stage of the US COVID-19 pandemic","volume":"3","author":"Zhang","year":"2020"},{"key":"b5","series-title":"Lancet","first-page":"514","article-title":"A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: A study of a family cluster","volume":"395","author":"Chan","year":"2020"},{"key":"b6","series-title":"Science","first-page":"571","article-title":"Cryptic transmission of SARS-CoV-2 in Washington state","volume":"370","author":"Bedford","year":"2020"},{"key":"b7","series-title":"Clin Infect Dis","first-page":"1776","article-title":"Household transmission of severe acute respiratory syndrome coronavirus 2 in the United States: Living density, viral load, and disproportionate impact on communities of color","volume":"74","author":"Cerami","year":"2022"},{"key":"b8","series-title":"Bioinformatics","first-page":"4121","article-title":"Nextstrain: Real-time tracking of pathogen evolution","volume":"34","author":"Hadfield","year":"2018"},{"key":"b9","series-title":"Sci Transl Med","first-page":"eabg4262","article-title":"Key epidemiological drivers and impact of interventions in the 2020 SARS-CoV-2 epidemic in England","volume":"13","author":"Knock","year":"2021"},{"key":"b10","series-title":"Bull Math Biol","first-page":"118","article-title":"Optimal control of the COVID-19 pandemic with non-pharmaceutical interventions","volume":"82","author":"Perkins","year":"2020"},{"key":"b11","series-title":"Science","first-page":"413","article-title":"The impact of COVID-19 and strategies for mitigation and suppression in low- and middle-income countries","volume":"369","author":"Walker","year":"2020"},{"key":"b12","series-title":"PLOS Comput Biol","first-page":"e1009374","article-title":"Using test positivity and reported case rates to estimate state-level COVID-19 prevalence and seroprevalence in the United States","volume":"17","author":"Chiu","year":"2021"},{"key":"b13","series-title":"Viruses","first-page":"2532","article-title":"Challenges and opportunities for global genomic surveillance strategies in the COVID-19 era","volume":"14","author":"Ling-Hu","year":"2022"},{"key":"b14","article-title":"WHO Global Genomic Surveillance Strategy for Pathogens with Pandemic and Epidemic Potential 2022\u20132032.","year":"2023"},{"key":"b15","series-title":"Malar J","first-page":"163","article-title":"Use cases for genetic epidemiology in malaria elimination","volume":"18","author":"Dalmat","year":"2019"},{"key":"b16","series-title":"Lancet Microbe","first-page":"100920","article-title":"Prevalence of mutations associated with artemisinin partial resistance and sulfadoxine-pyrimethamine resistance in 13 regions in Tanzania in 2021: A cross-sectional survey","volume":"5","author":"Juliano","year":"2024"},{"key":"b17","series-title":"Lancet Infect Dis","first-page":"1225","article-title":"Evidence of artemisinin partial resistance in northwestern Tanzania: Clinical and molecular markers of resistance","volume":"24","author":"Ishengoma","year":"2024"},{"key":"b18","series-title":"Malar J","first-page":"597","article-title":"Whole genome sequencing of Plasmodium falciparum from dried blood spots using selective whole genome amplification","volume":"15","author":"Oyola","year":"2016"},{"key":"b19","series-title":"Nucleic Acids Res","first-page":"e21","article-title":"SeekDeep: Single-base resolution de novo clustering for amplicon deep sequencing","volume":"46","author":"Hathaway","year":"2018"},{"key":"b20","series-title":"Front Parasitol","first-page":"1509261","article-title":"Application of a new highly multiplexed amplicon sequencing tool to evaluate Plasmodium falciparum antimalarial resistance and relatedness in individual and pooled samples from Dschang, Cameroon","volume":"3","author":"Sadler","year":"2025"},{"key":"b21","series-title":"Nat Commun","first-page":"3699","article-title":"Multiplexed ddPCR-amplicon sequencing reveals isolated Plasmodium falciparum populations amenable to local elimination in Zanzibar, Tanzania","volume":"14","author":"Holzschuh","year":"2023"},{"key":"b22","series-title":"Mol Ecol Resour","first-page":"2285","article-title":"Design and implementation of multiplexed amplicon sequencing panels to serve genomic epidemiology of infectious disease: A malaria case study","volume":"22","author":"LaVerriere","year":"2022"},{"key":"b23","series-title":"Sci Rep","first-page":"10737","article-title":"Sensitive and modular amplicon sequencing of Plasmodium falciparum diversity and resistance for research and public health","volume":"15","author":"Aranda-D\u00edaz","year":"2025"},{"key":"b24","series-title":"J Infect Dis","first-page":"1227","article-title":"Sensitive, highly multiplexed sequencing of microhaplotypes from the Plasmodium falciparum heterozygome","volume":"225","author":"Tessema","year":"2022"},{"key":"b25","series-title":"Nat Commun","first-page":"2107","article-title":"The impact of antimalarial resistance on the genetic structure of Plasmodium falciparum in the DRC","volume":"11","author":"Verity","year":"2020"},{"key":"b26","series-title":"J Infect Dis","first-page":"946","article-title":"Drug-resistance and population structure of 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infections","volume":"18","author":"Lerch","year":"2017"},{"key":"b31","series-title":"J Infect Dis","first-page":"580","article-title":"Use of massively parallel pyrosequencing to evaluate the diversity of and selection on Plasmodium falciparum csp T-cell epitopes in Lilongwe, Malawi","volume":"206","author":"Bailey","year":"2012"},{"key":"b32","series-title":"Lancet Infect Dis","first-page":"e591","article-title":"The emergence of artemisinin partial resistance in Africa: How do we respond?","volume":"24","author":"Rosenthal","year":"2024"},{"key":"b33","series-title":"Nat Rev Microbiol","first-page":"385","article-title":"Emergence, transmission dynamics and mechanisms of artemisinin partial resistance in malaria parasites in Africa","volume":"22","author":"Rosenthal","year":"2024"},{"key":"b34","series-title":"Nat Med","first-page":"1807","article-title":"Urgent action is needed to confront artemisinin partial resistance in African malaria parasites","volume":"30","author":"Ishengoma","year":"2024"},{"key":"b35","series-title":"J Infect Dis","first-page":"jiaf188","article-title":"Emergence and rising prevalence of artemisinin partial resistance marker kelch13 P441L in a low malaria transmission setting in Southern Zambia","volume":"2025","author":"Martin","year":"2025"},{"key":"b36","doi-asserted-by":"crossref","DOI":"10.1101\/2024.09.11.612449","article-title":"Multiplexed Nanopore Amplicon Sequencing to Distinguish Recrudescence From New Infection in Antimalarial Drug Trials.","author":"Holzschuh","year":"2024"},{"key":"b37","series-title":"Nat Microbiol","first-page":"1911","article-title":"Plasmodium falciparum resistant to artemisinin and diagnostics have emerged in Ethiopia","volume":"8","author":"Fola","year":"2023"},{"key":"b38","series-title":"Emerg Infect Dis","first-page":"462","article-title":"Major threat to malaria control programs by Plasmodium falciparum lacking histidine-rich protein 2, Eritrea","volume":"24","author":"Berhane","year":"2018"},{"key":"b39","series-title":"Nat Microbiol","first-page":"1289","article-title":"Plasmodium falciparum is evolving to escape malaria rapid diagnostic tests in Ethiopia","volume":"6","author":"Feleke","year":"2021"},{"key":"b40","series-title":"Bull World Health Organ","first-page":"558","article-title":"Prevalence of Plasmodium falciparum lacking histidine-rich proteins 2 and 3: A systematic review","volume":"98","author":"Thomson","year":"2020"},{"key":"b41","series-title":"Nat Comput Sci","first-page":"773","article-title":"Seamless sharing and peer review of code","volume":"2","year":"2022"},{"key":"b42","series-title":"Am J Epidemiol","first-page":"658","article-title":"Toward open and reproducible epidemiology","volume":"192","author":"Mathur","year":"2023"},{"key":"b43","series-title":"Am J Epidemiol","first-page":"783","article-title":"Reproducible epidemiologic research","volume":"163","author":"Peng","year":"2006"},{"key":"b44","series-title":"Sci Data","first-page":"160018","article-title":"The FAIR Guiding Principles for scientific data management and stewardship","volume":"3","author":"Wilkinson","year":"2016"},{"key":"b45","series-title":"Nat Med","first-page":"917","article-title":"Antimalarial drug resistance: Linking Plasmodium falciparum parasite biology to the clinic","volume":"23","author":"Blasco","year":"2017"},{"key":"b46","series-title":"Trends Parasitol","first-page":"537","article-title":"Recent highlights in antimalarial drug resistance and chemotherapy research","volume":"24","author":"Fidock","year":"2008"},{"key":"b47","series-title":"Curr Epidemiol Rep","first-page":"46","article-title":"Antimalarial drug resistance and implications for the WHO global technical strategy","volume":"8","author":"Ippolito","year":"2021"},{"key":"b48","series-title":"Lancet Infect Dis","first-page":"e338","article-title":"Antimalarial drug resistance in Africa: The calm before the storm?","volume":"19","author":"Conrad","year":"2019"},{"key":"b49","series-title":"Malar J","first-page":"89","article-title":"A systematic review and meta-analysis of evidence for correlation between molecular markers of parasite resistance and treatment outcome in falciparum malaria","volume":"8","author":"Picot","year":"2009"},{"key":"b50","series-title":"Bioinformatics","first-page":"3243","article-title":"Panoptes: Web-based exploration of large scale genome variation data","volume":"33","author":"Vauterin","year":"2017"},{"key":"b51","series-title":"Commun Med (Lond)","first-page":"97","article-title":"Household-level effects of seasonal malaria chemoprevention in the Gambia","volume":"4","author":"Soremekun","year":"2024"},{"key":"b52","series-title":"Am J Trop Med Hyg","first-page":"20","article-title":"Systematic review and meta-analysis of seasonal malaria chemoprevention","volume":"110","author":"Thwing","year":"2024"},{"key":"b53","series-title":"BMC Infect Dis","first-page":"872","article-title":"The changing landscape of Plasmodium falciparum drug resistance in the Democratic Republic of Congo","volume":"19","author":"Deutsch-Feldman","year":"2019"},{"key":"b54","series-title":"Trop Med Int Health","first-page":"1294","article-title":"Malaria in school-age children in Africa: An increasingly important challenge","volume":"19","author":"Nankabirwa","year":"2014"},{"key":"b55","series-title":"Malar J","first-page":"22","article-title":"Gender difference in the incidence of malaria diagnosed at public health facilities in Uganda","volume":"21","author":"Okiring","year":"2022"},{"key":"b56","series-title":"Elife","first-page":"e43510","article-title":"Using parasite genetic and human mobility data to infer local and cross-border malaria connectivity in Southern Africa","volume":"8","author":"Tessema","year":"2019"},{"key":"b57","article-title":"WHO External Quality Assurance Scheme for Malaria Nucleic Acid Amplification Testing.","year":"2023"},{"key":"b58","series-title":"Malar J","first-page":"129","article-title":"WHO malaria nucleic acid amplification test external quality assessment scheme: Results of distribution programmes one to three","volume":"19","author":"Cunningham","year":"2020"},{"key":"b59","series-title":"Trends Parasitol","first-page":"321","article-title":"Ahead of the curve: Next generation estimators of drug resistance in malaria infections","volume":"29","author":"Mideo","year":"2013"},{"key":"b60","article-title":"Towards an Open Analysis Ecosystem for Plasmodium Genomic Epidemiology.","author":"Ruybal-Pes\u00e1ntez","year":"2025"},{"key":"b61","series-title":"BMC Med Res Methodol","first-page":"10","article-title":"Overstating the evidence: Double counting in meta-analysis and related 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