{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T17:10:09Z","timestamp":1780333809161,"version":"3.54.1"},"reference-count":168,"publisher":"American Society of Tropical Medicine and Hygiene","license":[{"start":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T00:00:00Z","timestamp":1773705600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,17]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    malaria remains a leading cause of mortality in Angola, with emerging antimalarial resistance threatening treatment and prevention strategies. Efficacy of artemether\u2013lumefantrine has been reported below 90% in two provinces, underscoring the need for routine resistance surveillance. This study aimed to provide a geographically comprehensive and up-to-date overview of antimalarial drug resistance markers in Angola. Between March and July 2023, dried blood spots and demographic data were collected from\n                    <jats:italic>P<\/jats:italic>\n                    .\n                    <jats:italic>falciparum<\/jats:italic>\n                    -positive participants at 14 health facilities across 7 provinces. Multiplexed amplicon sequencing was used to characterize single nucleotide polymorphisms in 12 genes linked with resistance, estimate allele frequencies, and detect coinfecting non-falciparum\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    species. Sequence data from 820 samples revealed significant geographic variation in resistance markers. In the southeast, artemisinin partial resistance markers (\n                    <jats:italic>k13<\/jats:italic>\n                    P574L, P441L) were detected at very low prevalence (&lt;0.1%), whereas the quintuple\n                    <jats:italic>dhps<\/jats:italic>\n                    \/\n                    <jats:italic>dhfr<\/jats:italic>\n                    haplotype, linked to sulfadoxine-pyrimethamine resistance, was very prevalent (&gt;35% of samples). In the northwest, the sextuple\n                    <jats:italic>dhps<\/jats:italic>\n                    \/\n                    <jats:italic>dhfr<\/jats:italic>\n                    haplotype, a marker of higher sulfadoxine-pyrimethamine resistance, was most prevalent in the Zaire province (14.2%). The chloroquine resistance marker\n                    <jats:italic>crt<\/jats:italic>\n                    C72\/V73\/M74I\/N75E\/K76T (CVIET) haplotype had a national prevalence of 17.7%, detected in over 48% of samples from the northern sites. The\n                    <jats:italic>mdr1<\/jats:italic>\n                    N86 genotype, linked to reduced lumefantrine susceptibility, was detected in 99.2% of samples. Coinfections of\n                    <jats:italic>P<\/jats:italic>\n                    .\n                    <jats:italic>falciparum<\/jats:italic>\n                    and non-falciparum species were rare, with no\n                    <jats:italic>Plasmodium vivax<\/jats:italic>\n                    coinfections detected. These findings highlight the need for continued monitoring to safeguard treatment efficacy, reinforcing the importance of molecular surveillance in malaria control strategies.\n                  <\/jats:p>","DOI":"10.4269\/ajtmh.25-0226","type":"journal-article","created":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T15:12:26Z","timestamp":1773760346000},"source":"Crossref","is-referenced-by-count":1,"title":["Geographical Heterogeneity in Antimalarial Resistance Markers by Genomic Surveillance in Angola, 2023"],"prefix":"10.4269","author":[{"given":"Maria Florinda","family":"Jo\u00e3o","sequence":"first","affiliation":[{"name":"Programa Nacional do Controlo da Mal\u00e1ria, Luanda, Angola;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s","family":"Aranda-D\u00edaz","sequence":"additional","affiliation":[{"name":"EPPIcenter Research Program, Department of Medicine, University of California, San Francisco, California;"},{"name":"Malaria Elimination Initiative, Global Health Group, University of California, San Francisco, California;"},{"name":"ISGlobal Barcelona, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faith","family":"De Amaral","sequence":"additional","affiliation":[{"name":"EPPIcenter Research Program, Department of Medicine, University of California, San Francisco, California;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takalani I.","family":"Makhanthisa","sequence":"additional","affiliation":[{"name":"Laboratory for Antimalarial Resistance Monitoring and Malaria Operational Research, Centre of Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases, Johannesburg, South Africa;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonja B.","family":"Lauterbach","sequence":"additional","affiliation":[{"name":"Laboratory for Antimalarial Resistance Monitoring and Malaria Operational Research, Centre of Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases, Johannesburg, South Africa;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mukosha","family":"Chisenga","sequence":"additional","affiliation":[{"name":"SADC Malaria Elimination Eight Secretariat, Windhoek, Namibia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brighton","family":"Mangena","sequence":"additional","affiliation":[{"name":"SADC Malaria Elimination Eight Secretariat, Windhoek, Namibia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo","family":"Maquina","sequence":"additional","affiliation":[{"name":"SADC Malaria Elimination Eight Secretariat, Windhoek, Namibia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isobel","family":"Routledge","sequence":"additional","affiliation":[{"name":"Malaria Elimination Initiative, Global Health Group, University of California, San Francisco, California;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chadwick","family":"Sikaala","sequence":"additional","affiliation":[{"name":"SADC Malaria Elimination Eight Secretariat, Windhoek, Namibia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Chimumbwa","sequence":"additional","affiliation":[{"name":"SADC Malaria Elimination Eight Secretariat, Windhoek, Namibia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domingos","family":"Jandondo","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Investiga\u00e7\u00e3o em Sa\u00fade, Luanda, Angola;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Franco","family":"Martins","sequence":"additional","affiliation":[{"name":"Programa Nacional do Controlo da Mal\u00e1ria, Luanda, Angola;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaishree","family":"Raman","sequence":"additional","affiliation":[{"name":"Laboratory for Antimalarial Resistance Monitoring and Malaria Operational Research, Centre of Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases, Johannesburg, South Africa;"},{"name":"Wits Research Institute for Malaria, University of Witwatersrand, Johannesburg, South Africa;"},{"name":"University of Pretoria Institute for Sustainable Malaria Control, University of Pretoria, Pretoria, South Africa;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer L.","family":"Smith","sequence":"additional","affiliation":[{"name":"Malaria Elimination Initiative, Global Health Group, University of California, San Francisco, California;"},{"name":"Department of Epidemiology & Biostatistics, University of California, San Francisco, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro Rafael","family":"Dimbu","sequence":"additional","affiliation":[{"name":"Programa Nacional do Controlo da Mal\u00e1ria, Luanda, Angola;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","article-title":"World Malaria Report 2024. 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