{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T10:50:52Z","timestamp":1787655052107,"version":"build-2736575974"},"reference-count":40,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,10,1]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Helminthiases are common neglected tropical diseases in Thailand; thus, regular surveillance is necessary for their control. Among these helminthiases, the small liver fluke, <jats:italic>Opisthorchis viverrini<\/jats:italic> (<jats:italic>O. viverrini<\/jats:italic>), which is endemic in northeast Thailand, has been the focus of national control and eradication campaigns in the country for several decades. However, these helminths still exist and raise concerns regarding public health. Stool samples, blood samples, and demographic data were obtained from 307 participants. Stool examination via the formalin\u2013ethyl acetate concentration technique (FECT) and agar plate culture (APC) was performed, and parasite eggs and larvae were identified microscopically by experienced technicians. Of 307 individuals, 91 (29.6%) were positive for intestinal helminths and protozoans according to FECT and APC testing. Eggs of the <jats:italic>O. viverrini<\/jats:italic> species were the most commonly found (16% of participants), followed by <jats:italic>Strongyloides stercoralis<\/jats:italic> (14%), hookworms (1%), <jats:italic>Taenia<\/jats:italic> spp. (0.7%) and <jats:italic>Blastocystis<\/jats:italic> sp. (0.97%). The positive rates of anti-<jats:italic>Opisthorchis viverrini<\/jats:italic> IgG antibody, as evaluated via the immunochromatographic test (ICT) and ELISA, were 26.1% and 31.6%, respectively. The differences in positive results between the FECT (<jats:italic>O. viverrini<\/jats:italic> egg-positive) and anti-<jats:italic>O. viverrini<\/jats:italic> IgG antibody detected via ICT or ELISA were statistically significant (<jats:italic>P<\/jats:italic> &lt;0.05). The ICT and ELISA could possibly be used as the screening tools of choice for the diagnosis of human opisthorchiasis to aid in future treatment, prevention, and control measures.<\/jats:p>","DOI":"10.4269\/ajtmh.25-0258","type":"journal-article","created":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T14:24:24Z","timestamp":1754403864000},"page":"821-826","source":"Crossref","is-referenced-by-count":2,"title":["Current Prevalence of Infection with Opisthorchis viverrini and Intestinal Parasites in Rural Communities in Northeast Thailand Using Stool Examinations and Serological Screening Methods"],"prefix":"10.4269","volume":"113","author":[{"given":"Rutchanee","family":"Rodpai","sequence":"first","affiliation":[{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;"},{"name":"Department of Medical Technology, Faculty of Allied Health Sciences, Nakhonratchasima College, Nakhon Ratchasima, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Penchom","family":"Janwan","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lakkhana","family":"Sadaow","sequence":"additional","affiliation":[{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;"},{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oranuch","family":"Sanpool","sequence":"additional","affiliation":[{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;"},{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patcharaporn","family":"Boonroumkaew","sequence":"additional","affiliation":[{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;"},{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tassanee","family":"Saovana","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, Faculty of Allied Health Sciences, Nakhonratchasima College, Nakhon Ratchasima, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pokkamol","family":"Laoraksawong","sequence":"additional","affiliation":[{"name":"Faculty of Public Health, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pewpan M.","family":"Intapan","sequence":"additional","affiliation":[{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;"},{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanchai","family":"Maleewong","sequence":"additional","affiliation":[{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;"},{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weerachai","family":"Saijuntha","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Biomedical Science Research Unit, Mahasarakham University, Maha Sarakham, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tongjit","family":"Thanchomnang","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Biomedical Science Research Unit, Mahasarakham University, Maha Sarakham, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"J Clin Invest","first-page":"1311","article-title":"Helminth infections: The great neglected tropical diseases","volume":"118","author":"Hotez","year":"2008"},{"key":"b2","article-title":"Working to Overcome the Global Impact of Neglected Tropical Diseases: First WHO Report on Neglected Tropical Diseases.","year":"2021"},{"key":"b3","series-title":"Acta Trop","first-page":"106082","article-title":"Current status of helminthiases in Thailand: A cross-sectional, nationwide survey, 2019","volume":"223","author":"Wattanawong","year":"2021"},{"key":"b4","series-title":"Asian Biomed","first-page":"779","article-title":"Brief communication (Original). 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