{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T13:47:13Z","timestamp":1785937633520,"version":"3.56.0"},"reference-count":77,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,5]]},"abstract":"ABSTRACT.<\/jats:title>\n \n Ticks are obligate hematophagous arthropods at all developmental stages, primarily feeding on terrestrial vertebrates. They serve as vectors for numerous pathogens affecting both humans and animals. Accurate tick species identification currently requires an integrated approach combining morphological, molecular, and proteomic techniques. As part of a tick-borne disease surveillance program conducted in 2023 in the Kaffrine and Tambacounda regions of Senegal, ticks were collected from livestock in domestic environments to investigate the presence of\n Rickettsia<\/jats:italic>\n spp. and other associated microorganisms. Specimens were identified using morphological keys, molecular markers, and matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry (MALDI-TOF MS), while pathogens were detected using quantitative PCR (qPCR) and sequencing. A total of 212 ticks were collected, representing three genera (\n Amblyomma<\/jats:italic>\n ,\n Hyalomma<\/jats:italic>\n , and\n Rhipicephalus<\/jats:italic>\n ) and six species:\n Amblyomma variegatum<\/jats:italic>\n (\n n<\/jats:italic>\n = 60),\n Hyalomma rufipes<\/jats:italic>\n (\n n<\/jats:italic>\n = 52),\n Hyalomma truncatum<\/jats:italic>\n (\n n<\/jats:italic>\n = 22),\n Rhipicephalus evertsi evertsi<\/jats:italic>\n (\n n<\/jats:italic>\n = 59),\n Rhipicephalus linnaei<\/jats:italic>\n (formerly\n Rh. sanguineus<\/jats:italic>\n tropical lineage) (\n n<\/jats:italic>\n = 9), and\n Rhipicephalus (Boophilus) decoloratus<\/jats:italic>\n (\n n<\/jats:italic>\n = 3). Among these, 36.8% (78\/212) tested positive for at least one microorganism.\n Rickettsia<\/jats:italic>\n spp. and\n Coxiella burnetii<\/jats:italic>\n were each detected in 7.4% (16\/212), while members of the\n Anaplasmataceae<\/jats:italic>\n family were found in 5.2% (11\/212). Specific identification confirmed the presence of\n Rickettsia africae<\/jats:italic>\n ,\n R. aeschlimannii<\/jats:italic>\n ,\n Anaplasma ovis<\/jats:italic>\n ,\n A. phagocytophilum<\/jats:italic>\n , \u201c\n Candidatus<\/jats:italic>\n Ehrlichia rustica\u201d, \u201c\n Candidatus<\/jats:italic>\n Ehrlichia urmitei\u201d,\n Theileria ovis<\/jats:italic>\n and\n T. mutans<\/jats:italic>\n . These findings highlight the necessity of combining identification methods to enhance taxonomic precision, especially for closely related or morphologically ambiguous specimens. The presence of zoonotic pathogens reinforces the need for improved surveillance and awareness among health professionals.\n <\/jats:p>","DOI":"10.4269\/ajtmh.25-0330","type":"journal-article","created":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T18:36:41Z","timestamp":1781635001000},"page":"299-309","source":"Crossref","is-referenced-by-count":0,"title":["Identification of Livestock Ticks and Molecular Characterization of Tick-Associated Microorganisms in Senegal"],"prefix":"10.4269","volume":"115","author":[{"given":"Adama Zan","family":"Diarra","sequence":"first","affiliation":[{"name":"Institut de recherche pour le d\u00e9veloppement (IRD), Maladies Infectieuses, N\u00e9glig\u00e9es et Emergentes au Sud (MINES), S\u00e9n\u00e9gal;"},{"name":"Aix-Marseille Universit\u00e9, SSA, RITMES, Marseille, France;"},{"name":"IHU-M\u00e9diterran\u00e9e Infection, Marseille, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massamba","family":"Sylla","sequence":"additional","affiliation":[{"name":"Laboratoire Vecteurs & Parasites, D\u00e9partement Sciences et Techniques de l\u2019Elevage, Universit\u00e9 du Sine Saloum El Hadji Ibrahima NIASS (USSEIN), Kaffrine, S\u00e9n\u00e9gal;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khalifa Serigne Babacar","family":"Sylla","sequence":"additional","affiliation":[{"name":"Laboratoire Vecteurs & Parasites, D\u00e9partement Sciences et Techniques de l\u2019Elevage, Universit\u00e9 du Sine Saloum El Hadji Ibrahima NIASS (USSEIN), Kaffrine, S\u00e9n\u00e9gal;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georges","family":"Diatta","sequence":"additional","affiliation":[{"name":"Aix-Marseille Universit\u00e9, IRD, MINES, Campus International IRD-UCAD, Dakar, S\u00e9n\u00e9gal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ndeye Coumba Toure","family":"Kane","sequence":"additional","affiliation":[{"name":"Laboratoire Vecteurs & Parasites, D\u00e9partement Sciences et Techniques de l\u2019Elevage, Universit\u00e9 du Sine Saloum El Hadji Ibrahima NIASS (USSEIN), Kaffrine, S\u00e9n\u00e9gal;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheikh","family":"Sokhna","sequence":"additional","affiliation":[{"name":"Institut de recherche pour le d\u00e9veloppement (IRD), Maladies Infectieuses, N\u00e9glig\u00e9es et Emergentes au Sud (MINES), S\u00e9n\u00e9gal;"},{"name":"Aix-Marseille Universit\u00e9, SSA, RITMES, Marseille, France;"},{"name":"IHU-M\u00e9diterran\u00e9e Infection, Marseille, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Parola","sequence":"additional","affiliation":[{"name":"Aix-Marseille Universit\u00e9, SSA, RITMES, Marseille, France;"},{"name":"IHU-M\u00e9diterran\u00e9e Infection, Marseille, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"issue":"10","key":"b1","series-title":"Trends Parasitol","first-page":"437","article-title":"Ticks and tick-borne diseases: A One Health perspective","volume":"28","author":"Dantas-Torres","year":"2012"},{"issue":"6","key":"b2","series-title":"Clin Infect Dis","first-page":"897","article-title":"Ticks and tickborne bacterial diseases in humans: An emerging infectious threat","volume":"32","author":"Parola","year":"2001"},{"key":"b3","series-title":"Parasitology","first-page":"S3","article-title":"The global importance of ticks","volume":"129","author":"Jongejan","year":"2004"},{"issue":"2","key":"b4","series-title":"Med Trop (Mars)","first-page":"119","article-title":"Ticks and human tick-borne diseases in Africa","volume":"68","author":"Socolovschi","year":"2008"},{"issue":"5","key":"b5","series-title":"Ticks Tick Borne Dis","first-page":"101756","article-title":"Cattle ticks and tick-borne 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