{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:31:59Z","timestamp":1792801919730,"version":"4.5.0"},"reference-count":60,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,1]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n                  <jats:p>\n                    Zoonotic diseases account for more than 60% of emerging infectious diseases, and they are the leading cause of pandemics. As humans and livestock become increasingly transient and the environment and climate change, disease vectors expand into previously untouched geographical regions, spreading pathogens. In this study, we assessed the seroprevalence of arthropod-borne and hemorrhagic fever viruses of similar clinical presentation and endemicity in high-risk populations in Ghana (animal handlers). Using a microneutralization assay, we compared total IgG prevalence with live virus neutralizing response. In total, 300 blood samples were collected from consenting healthy adults at five military and three civilian sites across Ghana. The observed seroprevalence rates for Rift Valley fever virus (RVFV), Crimean\u2013Congo hemorrhagic fever virus (CCHFV), Ebola virus, Lassa virus, and Marburg virus were 14.7%, 7.0%, 2.3%, 1%, and 0%, respectively. Microneutralization data further verified virus-specific neutralization positives of the total IgG positives. Among animal handlers who had recently skinned livestock, 19 (25.3%) were exposed to RVFV, and 20 (28.6%) of those in the coastal savannah ecological zone were also more likely to be exposed to RVFV compared with those in the other ecological zones (\n                    <jats:italic>P<\/jats:italic>\n                    = 0.002). Animal handlers younger than 25 years old had a higher exposure rate to CCHFV than those older than 25 years old (\n                    <jats:italic>P<\/jats:italic>\n                    &lt;0.001). These data help us better understand the risk of exposure to zoonotic and vector-borne diseases in the region. Moreover, this study establishes methods for assessing seropositivity in a multiplexed format for higher-throughput sample analysis.\n                  <\/jats:p>","DOI":"10.4269\/ajtmh.25-0452","type":"journal-article","created":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T13:35:05Z","timestamp":1777988105000},"page":"145-152","source":"Crossref","is-referenced-by-count":1,"title":["Cross-Sectional Analysis of Serologic Response to Arthropod-Borne and Hemorrhagic Fever Viruses in Ghanaian Livestock Herders"],"prefix":"10.4269","volume":"115","author":[{"given":"Keersten","family":"Ricks","sequence":"first","affiliation":[{"name":"Diagnostic Systems Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Monticelli","sequence":"additional","affiliation":[{"name":"Virology Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland;"},{"name":"The Geneva Foundation, Tacoma, Washington;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seth Offei","family":"Addo","sequence":"additional","affiliation":[{"name":"Parasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara","family":"Clements","sequence":"additional","affiliation":[{"name":"Diagnostic Systems Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mba-Tihssommah","family":"Mosore","sequence":"additional","affiliation":[{"name":"Parasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald E.","family":"Bentil","sequence":"additional","affiliation":[{"name":"Parasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janice","family":"Tagoe","sequence":"additional","affiliation":[{"name":"Parasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Yeboah","sequence":"additional","affiliation":[{"name":"Parasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Behene","sequence":"additional","affiliation":[{"name":"Parasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Asiedu","sequence":"additional","affiliation":[{"name":"Public Health Division, Ghana Armed Forces, Accra, Ghana;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Mingle","sequence":"additional","affiliation":[{"name":"Public Health Division, Ghana Armed Forces, Accra, Ghana;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra Abankwa","family":"Kwarteng","sequence":"additional","affiliation":[{"name":"Department of Theoretical and Applied Biology, College of Science, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorcas","family":"Atibilla","sequence":"additional","affiliation":[{"name":"Kintampo Health Research Center, Bono East Region, Kintampo, Ghana;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor","family":"Asoala","sequence":"additional","affiliation":[{"name":"Navrongo Health Research Center, Upper East Region, Navrongo, Ghana;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Stefan","sequence":"additional","affiliation":[{"name":"Diagnostic Systems Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Herbert","sequence":"additional","affiliation":[{"name":"Virology Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terrel","family":"Sanders","sequence":"additional","affiliation":[{"name":"U.S. Naval Medical Research Unit\u2014European, Africa, and Central Commands (EURAFCENT), Ghana Detachment, Accra, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne T.","family":"Fox","sequence":"additional","affiliation":[{"name":"U.S. Naval Medical Research Unit\u2014European, Africa, and Central Commands (EURAFCENT), Ghana Detachment, Accra, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel K.","family":"Dadzie","sequence":"additional","affiliation":[{"name":"Parasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew G.","family":"Letizia","sequence":"additional","affiliation":[{"name":"U.S. Naval Medical Research Unit\u2014European, Africa, and Central Commands (EURAFCENT), Ghana Detachment, Accra, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randal","family":"Schoepp","sequence":"additional","affiliation":[{"name":"Diagnostic Systems Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shirley C.","family":"Nimo-Paintsil","sequence":"additional","affiliation":[{"name":"U.S. Naval Medical Research Unit\u2014European, Africa, and Central Commands (EURAFCENT), Ghana Detachment, Accra, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"issue":"10243","key":"b1","series-title":"Lancet","first-page":"1","article-title":"Zoonoses: Beyond the human\u2013animal\u2013environment interface","volume":"396","year":"2020"},{"issue":"9","key":"b2","series-title":"Microorganisms","first-page":"1405","article-title":"Zoonotic diseases: Etiology, impact, and control","volume":"8","author":"Rahman","year":"2020"},{"issue":"7181","key":"b3","series-title":"Nature","first-page":"990","article-title":"Global trends in emerging infectious diseases","volume":"451","author":"Jones","year":"2008"},{"issue":"5","key":"b4","series-title":"Lancet Infect Dis","first-page":"e108","article-title":"Disease X: Accelerating the development of medical countermeasures for the next pandemic","volume":"20","author":"Simpson","year":"2020"},{"issue":"8","key":"b5","series-title":"PLoS Pathog","first-page":"e1004992","article-title":"Interdisciplinarity and infectious diseases: An Ebola case study","volume":"11","author":"Ezenwa","year":"2015"},{"issue":"21","key":"b6","series-title":"N Engl J Med","first-page":"1969","article-title":"Developing Covid-19 vaccines at pandemic speed","volume":"382","author":"Lurie","year":"2020"},{"issue":"546","key":"b7","series-title":"Sci Transl Med","first-page":"eabc1931","article-title":"COVID-19 diagnostics in context","volume":"12","author":"Weissleder","year":"2020"},{"issue":"4","key":"b8","series-title":"Lancet Glob Health","first-page":"e579","article-title":"Strengthening global health security by improving disease surveillance in remote rural areas of low-income and middle-income countries","volume":"10","author":"Worsley-Tonks","year":"2022"},{"issue":"7","key":"b9","series-title":"Emerg Infect Dis","first-page":"1176","article-title":"Undiagnosed acute viral febrile illnesses, Sierra Leone","volume":"20","author":"Schoepp","year":"2014"},{"key":"b10","series-title":"Virol J","first-page":"314","article-title":"Capacity building permitting comprehensive monitoring of a severe case of Lassa hemorrhagic fever in Sierra Leone with a positive outcome: Case report","volume":"8","author":"Grove","year":"2011"},{"issue":"3","key":"b11","series-title":"Viruses","first-page":"790","article-title":"One Health surveillance highlights circulation of viruses with zoonotic potential in bats, pigs, and humans in Viet Nam","volume":"15","author":"Latinne","year":"2023"},{"key":"b12","series-title":"J Trop Med","first-page":"8938530","article-title":"Viral zoonoses of national importance in Ghana: Advancements and opportunities for enhancing capacities for early detection and response","volume":"2021","author":"Suu-Ire","year":"2021"},{"issue":"9","key":"b13","series-title":"PLoS Negl Trop Dis","first-page":"e821","article-title":"Tick-borne rickettsioses, neglected emerging diseases in rural Senegal","volume":"4","author":"Mediannikov","year":"2010"},{"issue":"3","key":"b14","series-title":"Open Forum Infect Dis","first-page":"ofz001","article-title":"Chikungunya and O\u2019nyong-nyong viruses in Uganda: Implications for diagnostics","volume":"6","author":"Clements","year":"2019"},{"key":"b15","series-title":"Anim Dis","first-page":"1","article-title":"First molecular identification of multiple tick-borne pathogens in livestock within Kassena-Nankana, Ghana","volume":"3","author":"Addo","year":"2023"},{"issue":"3","key":"b16","series-title":"Arch Virol","first-page":"62","article-title":"Molecular identification of Crimean\u2013Congo haemorrhagic fever virus in Hyalomma rufipes and Amblyomma variegatum in the Upper East Region of Ghana","volume":"169","author":"Addo","year":"2024"},{"issue":"1\u20132","key":"b17","series-title":"Exp Appl Acarol","first-page":"137","article-title":"Occurrence of Rickettsia spp. and Coxiella burnetii in ixodid ticks in Kassena-Nankana, Ghana","volume":"90","author":"Addo","year":"2023"},{"issue":"6","key":"b18","series-title":"Viruses","first-page":"1346","article-title":"Evidence of Rift Valley fever virus circulation in livestock and herders in southern Ghana","volume":"15","author":"Johnson","year":"2023"},{"key":"b19","series-title":"BMC Infect Dis","first-page":"324","article-title":"Crimean\u2013Congo hemorrhagic fever virus in livestock ticks and animal handler seroprevalence at an abattoir in Ghana","volume":"16","author":"Akuffo","year":"2016"},{"key":"b20","series-title":"Transbound Emerg Dis","first-page":"2063317","article-title":"First whole genome sequencing of Crimean\u2013Congo hemorrhagic fever virus (CCHFV) in tick species within Ghana","volume":"2023","author":"Bentil","year":"2023"},{"issue":"3","key":"b21","series-title":"J Infect Dis","first-page":"762","article-title":"A longitudinal analysis of memory immune responses in convalescent Crimean\u2013Congo hemorrhagic fever survivors in Uganda","volume":"231","author":"Cohen","year":"2025"},{"key":"b22","series-title":"Front Immunol","first-page":"1423474","article-title":"Serological cross-reactivity between Crimean\u2013Congo haemorrhagic fever virus and Nairobi sheep disease virus glycoprotein C","volume":"15","author":"Maze","year":"2024"},{"issue":"7","key":"b23","series-title":"Emerg Infect Dis","first-page":"1207","article-title":"Ebola virus antibodies in fruit bats, Ghana, West Africa","volume":"18","author":"Hayman","year":"2012"},{"issue":"11","key":"b24","series-title":"J Vet Med Sci","first-page":"1491","article-title":"Detection of anti-ebolavirus antibodies in Ghanaian pigs","volume":"84","author":"Ogawa","year":"2022"},{"issue":"25","key":"b25","series-title":"N Engl J Med","first-page":"2393","article-title":"Marburg virus disease in Ghana","volume":"388","author":"Bonney","year":"2023"},{"issue":"3","key":"b26","series-title":"Ghana Med J","first-page":"166","article-title":"The first cases of Lassa fever in Ghana","volume":"46","author":"Dzotsi","year":"2012"},{"key":"b27","article-title":"Weekly Bulletin on Outbreaks and Other Emergencies. Week 10: 27 February to 5 March 2023","year":"2023"},{"issue":"10","key":"b28","series-title":"Microorganisms","first-page":"1914","article-title":"Arbovirus epidemiology: The mystery of unnoticed epidemics in Ghana, West Africa","volume":"10","author":"Agboli","year":"2022"},{"key":"b29","series-title":"Front Cell Infect Microbiol","first-page":"975398","article-title":"Serological cross-reactivity among common flaviviruses","volume":"12","author":"Chan","year":"2022"},{"issue":"12","key":"b30","series-title":"Vaccines (Basel)","first-page":"1376","article-title":"Overview of neutralizing antibodies and their potential in COVID-19","volume":"9","author":"Morales-N\u00fa\u00f1ez","year":"2021"},{"issue":"10243","key":"b1","series-title":"Lancet","first-page":"1","article-title":"Zoonoses: Beyond the human\u2013animal\u2013environment interface","volume":"396","year":"2020"},{"issue":"9","key":"b2","series-title":"Microorganisms","first-page":"1405","article-title":"Zoonotic diseases: Etiology, impact, and control","volume":"8","author":"Rahman","year":"2020"},{"issue":"7181","key":"b3","series-title":"Nature","first-page":"990","article-title":"Global trends in emerging infectious diseases","volume":"451","author":"Jones","year":"2008"},{"issue":"5","key":"b4","series-title":"Lancet Infect Dis","first-page":"e108","article-title":"Disease X: Accelerating the development of medical countermeasures for the next pandemic","volume":"20","author":"Simpson","year":"2020"},{"issue":"8","key":"b5","series-title":"PLoS Pathog","first-page":"e1004992","article-title":"Interdisciplinarity and infectious diseases: An Ebola case study","volume":"11","author":"Ezenwa","year":"2015"},{"issue":"21","key":"b6","series-title":"N Engl J Med","first-page":"1969","article-title":"Developing Covid-19 vaccines at pandemic speed","volume":"382","author":"Lurie","year":"2020"},{"issue":"546","key":"b7","series-title":"Sci Transl Med","first-page":"eabc1931","article-title":"COVID-19 diagnostics in context","volume":"12","author":"Weissleder","year":"2020"},{"issue":"4","key":"b8","series-title":"Lancet Glob Health","first-page":"e579","article-title":"Strengthening global health security by improving disease surveillance in remote rural areas of low-income and middle-income countries","volume":"10","author":"Worsley-Tonks","year":"2022"},{"issue":"7","key":"b9","series-title":"Emerg Infect Dis","first-page":"1176","article-title":"Undiagnosed acute viral febrile illnesses, Sierra Leone","volume":"20","author":"Schoepp","year":"2014"},{"key":"b10","series-title":"Virol J","first-page":"314","article-title":"Capacity building permitting comprehensive monitoring of a severe case of Lassa hemorrhagic fever in Sierra Leone with a positive outcome: Case report","volume":"8","author":"Grove","year":"2011"},{"issue":"3","key":"b11","series-title":"Viruses","first-page":"790","article-title":"One Health surveillance highlights circulation of viruses with zoonotic potential in bats, pigs, and humans in Viet Nam","volume":"15","author":"Latinne","year":"2023"},{"key":"b12","series-title":"J Trop Med","first-page":"8938530","article-title":"Viral zoonoses of national importance in Ghana: Advancements and opportunities for enhancing capacities for early detection and response","volume":"2021","author":"Suu-Ire","year":"2021"},{"issue":"9","key":"b13","series-title":"PLoS Negl Trop Dis","first-page":"e821","article-title":"Tick-borne rickettsioses, neglected emerging diseases in rural Senegal","volume":"4","author":"Mediannikov","year":"2010"},{"issue":"3","key":"b14","series-title":"Open Forum Infect Dis","first-page":"ofz001","article-title":"Chikungunya and O\u2019nyong-nyong viruses in Uganda: Implications for diagnostics","volume":"6","author":"Clements","year":"2019"},{"key":"b15","series-title":"Anim Dis","first-page":"1","article-title":"First molecular identification of multiple tick-borne pathogens in livestock within Kassena-Nankana, Ghana","volume":"3","author":"Addo","year":"2023"},{"issue":"3","key":"b16","series-title":"Arch Virol","first-page":"62","article-title":"Molecular identification of Crimean\u2013Congo haemorrhagic fever virus in Hyalomma rufipes and Amblyomma variegatum in the Upper East Region of Ghana","volume":"169","author":"Addo","year":"2024"},{"issue":"1\u20132","key":"b17","series-title":"Exp Appl Acarol","first-page":"137","article-title":"Occurrence of Rickettsia spp. and Coxiella burnetii in ixodid ticks in Kassena-Nankana, Ghana","volume":"90","author":"Addo","year":"2023"},{"issue":"6","key":"b18","series-title":"Viruses","first-page":"1346","article-title":"Evidence of Rift Valley fever virus circulation in livestock and herders in southern Ghana","volume":"15","author":"Johnson","year":"2023"},{"key":"b19","series-title":"BMC Infect Dis","first-page":"324","article-title":"Crimean\u2013Congo hemorrhagic fever virus in livestock ticks and animal handler seroprevalence at an abattoir in Ghana","volume":"16","author":"Akuffo","year":"2016"},{"key":"b20","series-title":"Transbound Emerg Dis","first-page":"2063317","article-title":"First whole genome sequencing of Crimean\u2013Congo hemorrhagic fever virus (CCHFV) in tick species within Ghana","volume":"2023","author":"Bentil","year":"2023"},{"issue":"3","key":"b21","series-title":"J Infect Dis","first-page":"762","article-title":"A longitudinal analysis of memory immune responses in convalescent Crimean\u2013Congo hemorrhagic fever survivors in Uganda","volume":"231","author":"Cohen","year":"2025"},{"key":"b22","series-title":"Front Immunol","first-page":"1423474","article-title":"Serological cross-reactivity between Crimean\u2013Congo haemorrhagic fever virus and Nairobi sheep disease virus glycoprotein C","volume":"15","author":"Maze","year":"2024"},{"issue":"7","key":"b23","series-title":"Emerg Infect Dis","first-page":"1207","article-title":"Ebola virus antibodies in fruit bats, Ghana, West Africa","volume":"18","author":"Hayman","year":"2012"},{"issue":"11","key":"b24","series-title":"J Vet Med Sci","first-page":"1491","article-title":"Detection of anti-ebolavirus antibodies in Ghanaian pigs","volume":"84","author":"Ogawa","year":"2022"},{"issue":"25","key":"b25","series-title":"N Engl J Med","first-page":"2393","article-title":"Marburg virus disease in Ghana","volume":"388","author":"Bonney","year":"2023"},{"issue":"3","key":"b26","series-title":"Ghana Med J","first-page":"166","article-title":"The first cases of Lassa fever in Ghana","volume":"46","author":"Dzotsi","year":"2012"},{"key":"b27","article-title":"Weekly Bulletin on Outbreaks and Other Emergencies. Week 10: 27 February to 5 March 2023","year":"2023"},{"issue":"10","key":"b28","series-title":"Microorganisms","first-page":"1914","article-title":"Arbovirus epidemiology: The mystery of unnoticed epidemics in Ghana, West Africa","volume":"10","author":"Agboli","year":"2022"},{"key":"b29","series-title":"Front Cell Infect Microbiol","first-page":"975398","article-title":"Serological cross-reactivity among common flaviviruses","volume":"12","author":"Chan","year":"2022"},{"issue":"12","key":"b30","series-title":"Vaccines (Basel)","first-page":"1376","article-title":"Overview of neutralizing antibodies and their potential in COVID-19","volume":"9","author":"Morales-N\u00fa\u00f1ez","year":"2021"}],"container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/115\/1\/article-p145.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/115\/1\/article-p145.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/115\/1\/article-p145.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T16:20:42Z","timestamp":1782922842000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/115\/1\/article-p145.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,1]]},"references-count":60,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.25-0452","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,1]]}}}