{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:32:56Z","timestamp":1787031176086,"version":"build-2736575974"},"reference-count":40,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","license":[{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,2,4]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n                  <jats:p>Malaria remains a significant global health burden, with \u223c263 million cases across 83 countries. It is essential to quickly and accurately detect cases to control the spread of the disease. Given the widespread use of malaria rapid diagnostic tests (mRDTs) for case management and surveillance, it is crucial to understand test reliability. Clarifying how mRDT results differ from real-time polymerase chain reaction (qPCR) test results, as well as the nature of additional variance by test manufacturer, will be useful for reducing measurement bias. After comparing three national standard mRDTs and a research mRDT with qPCR results from a 2021 cross-sectional study in Tanzania, differences were found in terms of age, sex, and regional malaria transmission rate. The research test underperformed overall, with poor sensitivity across transmission strata. After comparing the research mRDT to standard mRDTs, the odds ratios indicated that transmission intensity may affect mRDT agreement and diagnostic performance. These results offer pertinent information on test accuracy and decrease outcome misclassification for malaria prevalence.<\/jats:p>","DOI":"10.4269\/ajtmh.25-0460","type":"journal-article","created":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T18:57:22Z","timestamp":1764874642000},"page":"287-291","source":"Crossref","is-referenced-by-count":1,"title":["Varying Malaria Rapid Diagnostic Test Accuracy by Regional Transmission Level and Demographics in Tanzania"],"prefix":"10.4269","volume":"114","author":[{"given":"Danielle","family":"Wiener","sequence":"first","affiliation":[{"name":"Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Misago D.","family":"Seth","sequence":"additional","affiliation":[{"name":"National Institute for Medical Research, Dar es Salaam, Tanzania;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celine I.","family":"Mandara","sequence":"additional","affiliation":[{"name":"National Institute for Medical Research, Dar es Salaam, Tanzania;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rashid A.","family":"Madebe","sequence":"additional","affiliation":[{"name":"National Institute for Medical Research, Dar es Salaam, Tanzania;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zachary R.","family":"Popkin-Hall","sequence":"additional","affiliation":[{"name":"Institute for Global Health and Infectious Diseases, University of North Carolina, Chapel Hill, North Carolina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Giesbrecht","sequence":"additional","affiliation":[{"name":"Brown University, Providence, Rhode Island;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Bakari","sequence":"additional","affiliation":[{"name":"National Institute for Medical Research, Dar es Salaam, Tanzania;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatus","family":"Lyimo","sequence":"additional","affiliation":[{"name":"National Institute for Medical Research, Dar es Salaam, Tanzania;"},{"name":"Nelson Mandela African Institute of Science and Technology, Arusha, Tanzania;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dativa","family":"Pereus","sequence":"additional","affiliation":[{"name":"National Institute for Medical Research, Dar es Salaam, Tanzania;"},{"name":"Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filbert","family":"Francis","sequence":"additional","affiliation":[{"name":"National Institute for Medical Research, Dar es Salaam, Tanzania;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Mbwambo","sequence":"additional","affiliation":[{"name":"National Malaria Control Programme, Dodoma, Tanzania;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sijenunu","family":"Aaron","sequence":"additional","affiliation":[{"name":"National Malaria Control Programme, Dodoma, Tanzania;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdalah","family":"Lusasi","sequence":"additional","affiliation":[{"name":"National Malaria Control Programme, Dodoma, Tanzania;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samwel","family":"Lazaro","sequence":"additional","affiliation":[{"name":"National Malaria Control Programme, Dodoma, Tanzania;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy P.","family":"Sheahan","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan B.","family":"Parr","sequence":"additional","affiliation":[{"name":"Institute for Global Health and Infectious Diseases, University of North Carolina, Chapel Hill, North Carolina;"},{"name":"Curriculum in Genetics and Molecular Biology, School of Medicine, University of North Carolina, Chapel Hill, North Carolina;"},{"name":"Division of Infectious Diseases, School of Medicine, University of North Carolina, Chapel Hill, North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey A","family":"Bailey","sequence":"additional","affiliation":[{"name":"Brown University, Providence, Rhode Island;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deus S.","family":"Ishengoma","sequence":"additional","affiliation":[{"name":"National Institute for Medical Research, Dar es Salaam, Tanzania;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan J.","family":"Juliano","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina;"},{"name":"Institute for Global Health and Infectious Diseases, University of North Carolina, Chapel Hill, North Carolina;"},{"name":"Curriculum in Genetics and Molecular Biology, School of Medicine, University of North Carolina, Chapel Hill, North Carolina;"},{"name":"Division of Infectious Diseases, School of Medicine, University of North Carolina, Chapel Hill, North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","article-title":"World Malaria Report 2024.","year":"2024"},{"key":"b2","series-title":"Ann Afr Med","first-page":"470","article-title":"SD-Bioline malaria rapid diagnostic test performance and time to become negative after treatment of 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malaria","volume":"111","author":"Golden","year":"2024"},{"key":"b10","series-title":"Malar J","first-page":"265","article-title":"Comparison of three rapid diagnostic tests for Plasmodium falciparum diagnosis in Ghana","volume":"23","author":"Kayode","year":"2024"},{"key":"b11","series-title":"Diagnostics (Basel)","first-page":"262","article-title":"Assessment of the performance of lactate dehydrogenase-based rapid diagnostic test for malaria in Djibouti in 2022\u20132023","volume":"14","author":"Abdi Moussa","year":"2024"},{"key":"b12","series-title":"Malar J","first-page":"236","article-title":"Field performance of Plasmodium falciparum lactate dehydrogenase rapid diagnostic tests during a large histidine-rich protein 2 deletion survey in Ethiopia","volume":"21","author":"Feleke","year":"2022"},{"key":"b13","series-title":"J Infect Dis","first-page":"713","article-title":"High-sensitivity assays for Plasmodium falciparum infection by immuno-polymerase chain reaction detection of 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Children","volume":"22","author":"Orimadegun","year":"2023"},{"key":"b3","series-title":"Int J Infect Dis","first-page":"377","article-title":"Comparative analysis of four malaria diagnostic tools and implications for malaria treatment in southwestern Nigeria","volume":"108","author":"Oboh","year":"2021"},{"key":"b4","series-title":"Sci Rep","first-page":"8158","article-title":"Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions among patients enrolled at 100 health facilities throughout Tanzania: February to July 2021","volume":"14","author":"Rogier","year":"2024"},{"key":"b5","series-title":"Pan Afr Med J","first-page":"48","article-title":"Accuracy of clinical diagnosis and malaria rapid diagnostic test and its influence on the management of children with fever under reduced malaria burden in Misungwi district, Mwanza Tanzania","volume":"25","author":"Nkonya","year":"2016"},{"key":"b6","series-title":"PLoS One","first-page":"e0302840","article-title":"Comparative evaluation of 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