{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T21:32:01Z","timestamp":1775079121173,"version":"3.50.1"},"reference-count":40,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,4,1]]},"abstract":"ABSTRACT.<\/jats:title>\n \n Gram-negative pathogens are notable causes of diverse human infections. This study determined the antibiotic sensitivity profile of clinical isolates of gram-negative bacteria to provide insights into high-priority pathogens that are potential threats to public health in Ghana. Gram-negative isolates from urine, stool, wound, and blood samples of consenting participants were tested against 10 antibiotics using the Kirby\u2013Bauer disc diffusion method.\n Escherichia coli<\/jats:italic>\n and\n Klebsiella pneumoniae<\/jats:italic>\n were the predominant bacteria isolated from the study participants. Resistance to colistin (4%) and meropenem (20%) was less prevalent, whereas isolates resistant to ampicillin (94%), tigecycline (85.5%), and chloramphenicol (76%) were widespread in the study participants.\n Acinetobacter baumannii<\/jats:italic>\n exhibited the highest resistance phenotype among the clinical isolates of Gram-negative bacteria. Our data highlight the ineffectiveness of tigecycline, ampicillin, and chloramphenicol against several gram-negative pathogens isolated from the study participants. The utilization of meropenem and colistin should be strongly monitored to avoid misuse because they represent effective antibiotics for treatment of gram-negative infections.\n <\/jats:p>","DOI":"10.4269\/ajtmh.25-0627","type":"journal-article","created":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T18:00:18Z","timestamp":1771351218000},"page":"639-643","source":"Crossref","is-referenced-by-count":0,"title":["Local Surveillance Identifies High-Priority Gram-Negative Pathogens to Guide Antibiotic Stewardship in Ghana: A Cross-Sectional Study"],"prefix":"10.4269","volume":"114","author":[{"given":"Gloria","family":"Amegatcher","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory Sciences, School of Biomedical and Allied Health Sciences, University of Ghana, Korle-Bu, Accra, Ghana;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johnson","family":"Adu","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences, School of Biomedical and Allied Health Sciences, University of Ghana, Korle-Bu, Accra, Ghana;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edwina Wilma","family":"Arcton","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences, School of Biomedical and Allied Health Sciences, University of Ghana, Korle-Bu, Accra, Ghana;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esther N.","family":"Okine","sequence":"additional","affiliation":[{"name":"Central Laboratory Services, Korle-Bu Teaching Hospital, Korle-Bu, Accra, Ghana;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas Kwesi","family":"Dankwah","sequence":"additional","affiliation":[{"name":"Central Laboratory Services, Korle-Bu Teaching Hospital, Korle-Bu, Accra, Ghana;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary-Magdalene","family":"Osei","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University of Ghana Medical School, Korle-Bu, Accra, Ghana;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isaac Kwame","family":"Sraku","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University of Ghana Medical School, Korle-Bu, Accra, Ghana;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marjorie Ntiwaa","family":"Quarchie","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences, School of Biomedical and Allied Health Sciences, University of Ghana, Korle-Bu, Accra, Ghana;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrews Abedi","family":"Ofosu","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences, School of Biomedical and Allied Health Sciences, University of Ghana, Korle-Bu, Accra, Ghana;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent","family":"Amarh","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, University of Ghana Medical School, Korle-Bu, Accra, Ghana;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moses","family":"Banyeh","sequence":"additional","affiliation":[{"name":"Department of Biomedical Laboratory Science, School of Allied Health Sciences, University for Development Studies, Tamale, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric S.","family":"Donkor","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University of Ghana Medical School, Korle-Bu, Accra, Ghana;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Lancet","first-page":"1172","article-title":"Global burden of antimicrobial resistance and forecasts to 2050","volume":"404","author":"Kariuki","year":"2024"},{"key":"b2","article-title":"Antimicrobial Resistance: Accelerating National and Global Responses. WHO Strategic and Operational Priorities to Address Drug-resistant Bacterial Infections in the Human Health Sector, 2025\u20132035.","year":"2025"},{"key":"b3","series-title":"JAC Antimicrob Resist","first-page":"dlad062","article-title":"Drivers of inappropriate antibiotic use in low- and middle-income countries","volume":"5","author":"Otaigbe","year":"2023"},{"key":"b4","series-title":"BMC Prim Care","first-page":"287","article-title":"Non-prescribed antibiotic dispensing practices for symptoms of urinary tract infection in community pharmacies and accredited drug dispensing outlets in Tanzania: A simulated clients approach","volume":"23","author":"Ndaki","year":"2022"},{"key":"b5","series-title":"Medchemcomm","first-page":"12","article-title":"Narrow-spectrum antibacterial agents","volume":"9","author":"Melander","year":"2018"},{"key":"b6","series-title":"Antimicrobial Drug Resistance: Clinical and Epidemiological Aspects","first-page":"889","volume":"2","author":"Paterson","year":"2017"},{"key":"b7","series-title":"Biotechnol J","first-page":"e1700750","article-title":"Identification and antibiotic-susceptibility profiling of infectious bacterial agents: A review of current and future trends","volume":"14","author":"Maugeri","year":"2019"},{"key":"b8","series-title":"Sci Rep","first-page":"23195","article-title":"Genomic analysis of Enterobacteriaceae from colorectal cancer patients at a tertiary hospital in Ghana: A case-control study","volume":"14","author":"Bachelle","year":"2024"},{"key":"b9","series-title":"Performance Standards for Antimicrobial Susceptibility Testing; Twenty-First Informational Supplement. 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WHO Strategic and Operational Priorities to Address Drug-resistant Bacterial Infections in the Human Health Sector, 2025\u20132035.","year":"2025"},{"key":"b3","series-title":"JAC Antimicrob Resist","first-page":"dlad062","article-title":"Drivers of inappropriate antibiotic use in low- and middle-income countries","volume":"5","author":"Otaigbe","year":"2023"},{"key":"b4","series-title":"BMC Prim Care","first-page":"287","article-title":"Non-prescribed antibiotic dispensing practices for symptoms of urinary tract infection in community pharmacies and accredited drug dispensing outlets in Tanzania: A simulated clients approach","volume":"23","author":"Ndaki","year":"2022"},{"key":"b5","series-title":"Medchemcomm","first-page":"12","article-title":"Narrow-spectrum antibacterial agents","volume":"9","author":"Melander","year":"2018"},{"key":"b6","series-title":"Antimicrobial Drug Resistance: Clinical and Epidemiological Aspects","first-page":"889","volume":"2","author":"Paterson","year":"2017"},{"key":"b7","series-title":"Biotechnol J","first-page":"e1700750","article-title":"Identification and antibiotic-susceptibility profiling of infectious bacterial agents: A review of current and future trends","volume":"14","author":"Maugeri","year":"2019"},{"key":"b8","series-title":"Sci Rep","first-page":"23195","article-title":"Genomic analysis of Enterobacteriaceae from colorectal cancer patients at a tertiary hospital in Ghana: A case-control study","volume":"14","author":"Bachelle","year":"2024"},{"key":"b9","series-title":"Performance Standards for Antimicrobial Susceptibility Testing; Twenty-First Informational Supplement. 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