{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T14:57:57Z","timestamp":1791385077500,"version":"4.3.3"},"reference-count":43,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,7]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n                  <jats:p>Undiagnosed acute febrile illness (AFI) remains elevated in the Peruvian Amazon. The contribution of Oropouche virus (OROV) to AFI in Loreto was quantified, and its recent circulation was contextualized using targeted molecular testing and phylogenomic analysis. Using a novel research-use-only OROV real-time quantitative polymerase chain reaction test across seven surveillance sites, 1,000 AFI samples were rescreened in a setting where undiagnosed presentations were common (70% lacked a confirmed etiology at enrollment). Oropouche virus was detected in 7.1% of cases, with nearly all consensus genomes recovered from specimens with a cycle threshold value \u226430, underscoring the importance of early-phase sampling for sequencing success. Positive results occurred at all seven locations, including the remote riverine community of San Lorenzo, indicating broad geographic distribution and supporting ongoing endemic transmission rather than a single localized outbreak. Exposure patterns were consistent with peridomestic, vector-mediated acquisition: animal contact was common (63.4%), whereas contact with symptomatic individuals (12.7%) and recent travel (35.7%) were infrequent, supporting local, not person-to-person or travel-related, transmission. Oropouche virus cases peaked in January to February 2024, temporally overlapping with the seasonal rise in AFIs, indicating synchronous circulation with other endemic pathogens. Phylogenetic arrangement of contemporary Peruvian strains within established South American diversity further supports ongoing local transmission consistent with regional patterns. Together, these findings indicate that OROV accounts for a meaningful portion of AFI in Loreto and that the routine inclusion of OROV testing, paired with genomic surveillance, can close diagnostic gaps, improve situational awareness, and guide targeted public health action.<\/jats:p>","DOI":"10.4269\/ajtmh.25-0640","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T14:30:43Z","timestamp":1785853843000},"page":"841-850","source":"Crossref","is-referenced-by-count":0,"title":["Oropouche Virus in Loreto, Peru (2023\u20132024): Case Distribution, Genome Sequencing, and Phylogenetic Insights"],"prefix":"10.4269","volume":"115","author":[{"given":"Katherine","family":"Torres","sequence":"first","affiliation":[{"name":"Laboratorio de Malaria, Laboratorios de Investigaci\u00f3n y Desarrollo, Facultad de Ciencias e Ingenieria, Universidad Peruana Cayetano Heredia, Lima, Peru;"},{"name":"Departamento de Ciencias Celulares y Moleculares, Facultad de Ciencias e Ingenier\u00eda, Universidad Peruana Cayetano Heredia, Lima, Peru;"},{"name":"Instituto de Medicina Tropical \u201cAlexander von Humboldt\u201d, Universidad Peruana Cayetano Heredia, Lima, Peru;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alonso","family":"Cruz-Echevarria","sequence":"additional","affiliation":[{"name":"Laboratorio de Malaria, Laboratorios de Investigaci\u00f3n y Desarrollo, Facultad de Ciencias e Ingenieria, Universidad Peruana Cayetano Heredia, Lima, Peru;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lester J.","family":"Perez","sequence":"additional","affiliation":[{"name":"Discovery Research Abbott, Abbott Park, Illinois, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Cubas","sequence":"additional","affiliation":[{"name":"Laboratorio del Centro Internacional de Excelencia en Malaria \u2013 Enfermedades Emergentes, Laboratorios de Investigaci\u00f3n y Desarrollo, Facultad de Ciencias e Ingenier\u00eda, Universidad Peruana Cayetano Heredia, Lima, Peru;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jhon","family":"Zumaeta","sequence":"additional","affiliation":[{"name":"Laboratorio de Malaria: Par\u00e1sitos y Vectores, Laboratorios de Investigaci\u00f3n y Desarrollo, Facultad de Ciencias e Ingenier\u00eda, Universidad Peruana Cayetano Heredia, Lima, Peru;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Freddy","family":"Alava","sequence":"additional","affiliation":[{"name":"Laboratorios Teodoro E. 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