{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T05:28:30Z","timestamp":1792214910065,"version":"4.4.0"},"reference-count":20,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,7]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n                  <jats:p>Orthobunyaviruses, one of the largest and most diverse genera of viruses, include numerous reassortant viruses and present diagnostic challenges due to similar clinical presentations with other arboviral infections. The detection and isolation of a group C orthobunyavirus from a patient with acute febrile illness from Puerto Ospina, Putumayo Department, Colombia during the 2023\u20132024 Latin American Oropouche fever regional outbreaks are reported in the present study. Using a vertebrate virus-focused metagenomics sequencing approach with VirCapSeq-VERT, a virus related to the Caraparu complex was identified, and a virus isolate was obtained. Although limited to a single case and without serological or longitudinal clinical confirmation, these findings underscore the value of unbiased molecular approaches for unresolved febrile illness diagnosis in endemic settings such as the Amazon basin.<\/jats:p>","DOI":"10.4269\/ajtmh.25-0652","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T16:50:53Z","timestamp":1787849453000},"page":"851-855","source":"Crossref","is-referenced-by-count":1,"title":["Group C Orthobunyavirus Infection in a Patient from the Amazon Putumayo Region of Colombia During an Oropouche Fever Epidemic"],"prefix":"10.4269","volume":"115","author":[{"given":"Sully","family":"M\u00e1rquez","sequence":"first","affiliation":[{"name":"Instituto de Microbiolog\u00eda, Universidad San Francisco de Quito, Quito, Ecuador;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lizeth","family":"Salazar","sequence":"additional","affiliation":[{"name":"Instituto de Microbiolog\u00eda, Universidad San Francisco de Quito, Quito, Ecuador;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Collins","sequence":"additional","affiliation":[{"name":"Global Alliance for Preventing Pandemics, Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Nipaz","sequence":"additional","affiliation":[{"name":"Instituto de Microbiolog\u00eda, Universidad San Francisco de Quito, Quito, Ecuador;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"D\u00e1vila Campos","sequence":"additional","affiliation":[{"name":"Hospital San Miguel, Quina Care Foundation, Sucumb\u00edos, Ecuador;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob","family":"Van der Ende","sequence":"additional","affiliation":[{"name":"Hospital San Miguel, Quina Care Foundation, Sucumb\u00edos, Ecuador;"},{"name":"Center for Tropical Medicine and Travel Medicine, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Kenneth","family":"Wickiser","sequence":"additional","affiliation":[{"name":"Global Alliance for Preventing Pandemics, Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, USA;"},{"name":"Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, New York, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Briese","sequence":"additional","affiliation":[{"name":"Global Alliance for Preventing Pandemics, Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, USA;"},{"name":"Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, New York, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josefina","family":"Coloma","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"issue":"10","key":"b1","series-title":"Nat Rev Microbiol","first-page":"673","article-title":"Orthobunyaviruses: Recent genetic and structural insights","volume":"12","author":"Elliott","year":"2014"},{"key":"b2","series-title":"Am J Trop Med Hyg","first-page":"250","article-title":"Group C, a new serological group of hitherto undescribed arthropod-borne viruses. Immunological studies","volume":"10","author":"Casals","year":"1961"},{"key":"b3","series-title":"Am J Trop Med Hyg","first-page":"283","article-title":"Further studies on the serological relationships of group C arthropod-borne viruses and the application of these relationships to rapid identification of types","volume":"11","author":"Shope","year":"1962"},{"key":"b4","series-title":"Am J Trop Med Hyg","first-page":"227","article-title":"The isolation of arthropod-borne viruses, including members of two hitherto undescribed serological groups, in the Amazon Region of Brazil","volume":"10","author":"Causey","year":"1961"},{"issue":"3","key":"b5","series-title":"PLoS One","first-page":"e92114","article-title":"Genomic characterization of group C orthobunyavirus reference strains and recent South American clinical isolates","volume":"9","author":"Hang","year":"2014"},{"issue":"7","key":"b6","series-title":"Lancet Infect Dis","first-page":"e439","article-title":"Emergence of Oropouche fever in Latin America: A narrative review","volume":"24","author":"Wesselmann","year":"2024"},{"issue":"9","key":"b7","series-title":"PLoS Negl Trop Dis","first-page":"e1315","article-title":"Iquitos virus: A novel reassortant orthobunyavirus associated with human illness in Peru","volume":"5","author":"Aguilar","year":"2011"},{"issue":"2","key":"b8","series-title":"Lancet Infect Dis","first-page":"166","article-title":"Re-emergence of Oropouche virus between 2023 and 2024 in Brazil: An observational epidemiological study","volume":"25","author":"Scachetti","year":"2025"},{"issue":"4","key":"b9","series-title":"J Infect","first-page":"106470","article-title":"Emergence of Oropouche virus infection in Matanzas, Cuba, 2024","volume":"90","author":"Bello-Rodr\u00edguez","year":"2025"},{"issue":"5","key":"b10","series-title":"Emerg Infect Dis","first-page":"935","article-title":"Isolation of Oropouche virus from febrile patient, Ecuador","volume":"24","author":"Wise","year":"2018"},{"key":"b11","series-title":"Int J Infect Dis","first-page":"108000","article-title":"Genomic evidence of active circulation of Orthobunyavirus in Ecuador","volume":"159","author":"Romero-Alvarez","year":"2025"},{"issue":"11","key":"b12","series-title":"Emerg Infect Dis","first-page":"2447","article-title":"Iquitos virus in traveler returning to the United States from Ecuador","volume":"30","author":"Baer","year":"2024"},{"issue":"7","key":"b13","series-title":"Emerg Infect Dis","first-page":"1295","article-title":"Single-reaction multiplex reverse transcription PCR for detection of Zika, chikungunya, and dengue viruses","volume":"22","author":"Waggoner","year":"2016"},{"issue":"1","key":"b14","series-title":"Diagn Microbiol Infect Dis","first-page":"114894","article-title":"Real-time RT-PCR for the detection and quantitation of Oropouche virus","volume":"96","author":"Rojas","year":"2020"},{"issue":"5","key":"b15","series-title":"mBio","first-page":"e01491","article-title":"Virome capture sequencing enables sensitive viral diagnosis and comprehensive virome analysis","volume":"6","author":"Briese","year":"2015"},{"issue":"5","key":"b16","series-title":"Am J Trop Med Hyg","first-page":"1019","article-title":"Oropouche virus: Clinical, epidemiological, and molecular aspects of a neglected orthobunyavirus","volume":"96","author":"Travassos da Rosa","year":"2017"},{"issue":"7","key":"b17","series-title":"PLoS One","first-page":"e0200576","article-title":"Molecular and antigenic characterization of group C orthobunyaviruses isolated in Peru","volume":"13","author":"Castillo Or\u00e9","year":"2018"},{"issue":"5","key":"b18","series-title":"PLoS One","first-page":"e0197294","article-title":"Revalidation and genetic characterization of new members of Group C (Orthobunyavirus genus, Peribunyaviridae family) isolated in the Americas","volume":"13","author":"Nunes","year":"2018"},{"issue":"1","key":"b19","series-title":"Emerg Infect Dis","first-page":"202","article-title":"Cocirculation of 4 dengue virus serotypes, Putumayo Amazon Basin, 2023\u20132024","volume":"31","author":"van der Ende","year":"2025"},{"issue":"4","key":"b20","series-title":"Emerg Infect Dis","first-page":"879","article-title":"Co-circulation of 2 Oropouche virus lineages during outbreak, Amazon Region of Peru, 2023\u20132024","volume":"31","author":"Cruz","year":"2025"}],"container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/115\/4\/article-p851.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/115\/4\/article-p851.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/115\/4\/article-p851.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T14:24:53Z","timestamp":1791383093000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/115\/4\/article-p851.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,7]]},"references-count":20,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.25-0652","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,7]]}}}