{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:57:20Z","timestamp":1790708240205,"version":"4.1.0"},"reference-count":21,"publisher":"American Society of Tropical Medicine and Hygiene","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,29]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n                  <jats:p>\n                    In Tunisia, toxoplasmosis screening in pregnancy is common but not regulated. This study evaluated congenital toxoplasmosis after primary infections during the periconceptional period and the accuracy of diagnostic tools. We analyzed 209 women at the Pasteur Institute of Tunis (2005\u20132021) with primary infections classified as periconceptional (\n                    <jats:italic>n<\/jats:italic>\n                    = 50, 23.9%), first trimester (\n                    <jats:italic>n<\/jats:italic>\n                    = 121, 57.9%), or second trimester (\n                    <jats:italic>n<\/jats:italic>\n                    = 38, 18.2%). Congenital toxoplasmosis was confirmed in 29 cases (13.9%), diagnosed prenatally in 22 cases, and diagnosed postnatally in 7 cases. Transmission rates were 14%, 8.2%, and 31.5% for periconceptional, first-trimester, and second-trimester infections, respectively, with significantly higher transmission in the second trimester (\n                    <jats:italic>P<\/jats:italic>\n                    = 0.048 versus periconceptional,\n                    <jats:italic>P<\/jats:italic>\n                    = 0.0003 versus first trimester). Prenatal diagnosis was positive in 42.8% of cases during the periconceptional period, 90% of cases during the first trimester, and 83.3% of cases during the second trimester, with false-negative polymerase chain reactions being more frequent during the periconceptional period (\n                    <jats:italic>P<\/jats:italic>\n                    &lt;0.05). Periconceptional infections pose a high risk of fetal transmission, requiring monitoring.\n                  <\/jats:p>","DOI":"10.4269\/ajtmh.25-0740","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:56:18Z","timestamp":1790704578000},"source":"Crossref","is-referenced-by-count":0,"title":["Toxoplasma Transmission to the Fetus during the Periconceptional Period"],"prefix":"10.4269","author":[{"given":"Rym","family":"Ben-Abdallah","sequence":"first","affiliation":[{"name":"Research Laboratory Medical Parasitology, Biotechnology and Biomolecule LR16-IPT-06, Pasteur Institue of Tunis, Tunis, Tunisia;"},{"name":"Department of Parasitology-Mycology, Pasteur Institute of Tunis, Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rihab","family":"Trabelsi","sequence":"additional","affiliation":[{"name":"Research Laboratory Medical Parasitology, Biotechnology and Biomolecule LR16-IPT-06, Pasteur Institue of Tunis, Tunis, Tunisia;"},{"name":"Department of Parasitology-Mycology, Pasteur Institute of Tunis, Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olfa","family":"Souissi","sequence":"additional","affiliation":[{"name":"Department of Parasitology-Mycology, Pasteur Institute of Tunis, Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rania","family":"Maatoug","sequence":"additional","affiliation":[{"name":"Department of Parasitology-Mycology, Pasteur Institute of Tunis, Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karim","family":"Aoun","sequence":"additional","affiliation":[{"name":"Research Laboratory Medical Parasitology, Biotechnology and Biomolecule LR16-IPT-06, Pasteur Institue of Tunis, Tunis, Tunisia;"},{"name":"Department of Parasitology-Mycology, Pasteur Institute of Tunis, Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aida","family":"Bouratbine","sequence":"additional","affiliation":[{"name":"Research Laboratory Medical Parasitology, Biotechnology and Biomolecule LR16-IPT-06, Pasteur Institue of Tunis, Tunis, Tunisia;"},{"name":"Department of Parasitology-Mycology, Pasteur Institute of Tunis, Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Food Waterborne Parasitol","first-page":"e00162","article-title":"Congenital toxoplasmosis: Should we still care about screening?","volume":"27","author":"Petersen","year":"2022"},{"issue":"3","key":"b2","series-title":"J Med Screen","first-page":"135","article-title":"Congenital toxoplasmosis in the United Kingdom: To screen or not to screen?","volume":"9","author":"Gilbert","year":"2002"},{"issue":"1","key":"b3","series-title":"Bull Soc Pathol Exot","first-page":"37","article-title":"\u00c9tat actuel de la toxoplasmose dans la r\u00e9gion de Sfax, Tunisie","volume":"103","author":"Sellami","year":"2010"},{"issue":"2","key":"b4","series-title":"Bull Soc Pathol Exot","first-page":"108","article-title":"D\u00e9pistage de la toxoplasmose materno-foetale: \u00e9tude des cas suivis \u00e0 l\u2019Institut Pasteur de Tunis (2007\u20132010)","volume":"106","author":"Ben Abdallah","year":"2013"},{"key":"b5","series-title":"Tunis Med","first-page":"85","article-title":"Difficult\u00e9s de la datation de la primo-infection par Toxoplasma gondii chez les parturientes en Tunisie","volume":"84","author":"Siala","year":"2006"},{"key":"b6","series-title":"Rev Tunis Infect","first-page":"104","article-title":"Diagnostic de la toxoplasmose cong\u00e9nitale au cours de la grossesse: R\u00e9sultats de l\u2019analyse par PCR de 97 liquides amniotiques","volume":"4","author":"Ben Abdallah","year":"2010"},{"key":"b7","series-title":"J Gynecol Obstet Biol Reprod","first-page":"546","article-title":"S\u00e9roconversion toxoplasmique p\u00e9riconceptionnelle: \u00e0 propos de 79 cas","volume":"41","author":"Garabedian","year":"2012"},{"issue":"10","key":"b8","series-title":"Gynecol Obstet Fertil Senol","first-page":"782","article-title":"Toxoplasmose pendant la grossesse: Proposition actuelle de prise en charge pratique","volume":"49","author":"Mandelbrot","year":"2021"},{"issue":"6","key":"b9","series-title":"Am J Trop Med Hyg","first-page":"1722","article-title":"Congenital toxoplasmosis in Tunisia: Prenatal and neonatal diagnosis and postnatal follow-up of 35 cases","volume":"98","author":"Boudaouara","year":"2018"},{"issue":"2","key":"b10","series-title":"Arch Pediatr","first-page":"118","article-title":"La toxoplasmose cong\u00e9nitale en Tunisie: Analyse clinique et biologique de 11 cas","volume":"16","author":"Ben Abdallah","year":"2009"},{"issue":"1","key":"b11","series-title":"Pathogens","first-page":"24","article-title":"Maternal and congenital toxoplasmosis: Diagnosis and treatment recommendations of a French multidisciplinary working group","volume":"8","author":"Peyron","year":"2019"},{"issue":"1","key":"b12","series-title":"Pathogens","first-page":"25","article-title":"Congenital toxoplasmosis: A plea for a neglected disease","volume":"7","author":"Wallon","year":"2018"},{"issue":"9167","key":"b13","series-title":"Lancet","first-page":"1829","article-title":"Mother-to-child transmission of toxoplasmosis: Risk estimates for clinical counselling","volume":"353","author":"Dunn","year":"1999"},{"issue":"11","key":"b14","series-title":"N Engl J Med","first-page":"695","article-title":"Prenatal diagnosis of congenital toxoplasmosis with a polymerase-chain-reaction test on amniotic fluid","volume":"331","author":"Hohlfeld","year":"1994"},{"issue":"8921","key":"b15","series-title":"Lancet","first-page":"541","article-title":"Toxoplasmosis in pregnancy","volume":"344","author":"Daffos","year":"1994"},{"key":"b16","article-title":"Diagnostic biologique de la toxoplasmose acquise du sujet immunocomp\u00e9tent (dont la femme enceinte), la toxoplasmose cong\u00e9nitale (diagnostic pr\u00e9- et postnatal) et la toxoplasmose oculaire.","year":"2017"},{"issue":"2","key":"b17","series-title":"Clin Microbiol Rev","first-page":"264","article-title":"Epidemiology of and diagnostic strategies for toxoplasmosis","volume":"25","author":"Robert-Gangneux","year":"2012"},{"key":"b18","series-title":"Pediatr Infect Dis J","article-title":"First case of congenital toxoplasmosis after maternal reinfection in Colombia: Serotyping evidence of reinfection by different strains","author":"Beltr\u00e1n-Higuera","year":"2026"},{"issue":"1","key":"b19","series-title":"Infect Immun","first-page":"418","article-title":"Tissue barriers of the human placenta to infection with Toxoplasma gondii.","volume":"80","author":"Robbins","year":"2012"},{"key":"b20","series-title":"Am J Obstet Gynecol","first-page":"843","article-title":"Prenatal diagnosis of congenital toxoplasmosis: A multicenter evaluation of different diagnostic parameters","volume":"181","author":"Foulon","year":"1999"},{"key":"b21","series-title":"Prenat Diagn","first-page":"558","article-title":"Fetal toxoplasmosis and negative amniocentesis: Necessity of an ultrasound follow-up","volume":"23","author":"Gay-Andrieu","year":"2003"}],"container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/aop\/article-10.4269-ajtmh.25-0740\/article-10.4269-ajtmh.25-0740.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/aop\/article-10.4269-ajtmh.25-0740\/article-10.4269-ajtmh.25-0740.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:57:00Z","timestamp":1790704620000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/aop\/article-10.4269-ajtmh.25-0740\/article-10.4269-ajtmh.25-0740.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,29]]},"references-count":21,"URL":"https:\/\/doi.org\/10.4269\/ajtmh.25-0740","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,29]]}}}