{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T15:08:59Z","timestamp":1780499339634,"version":"3.54.1"},"reference-count":42,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,3]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n                  <jats:p>\n                    Cutaneous leishmaniasis, which is caused by\n                    <jats:italic>Leishmania<\/jats:italic>\n                    species transmitted by sandflies, is increasingly recognized in subtropical, higher-resource regions, including the southern United States. The first whole-genome sequence of\n                    <jats:italic>Leishmania mexicana<\/jats:italic>\n                    (\n                    <jats:italic>L. mexicana<\/jats:italic>\n                    ) is reported from an autochthonous US case in a 3-year-old boy from Ellis County, Texas, with no travel history. Genomic DNA was sequenced using Illumina (San Diego, CA) technology. Phylogenomic analysis of nuclear and mitochondrial single-nucleotide polymorphisms (SNPs) confirmed the isolate as\n                    <jats:italic>L. mexicana<\/jats:italic>\n                    , clustering within the\n                    <jats:italic>L. mexicana<\/jats:italic>\n                    complex. The parasite displayed a predominantly disomic karyotype, with chromosome 30 exhibiting trisomy. A total of 172 genes were identified, with notable copy number variation and 9,920 SNPs unique to this genome, highlighting substantial genetic diversity. These variants affected genes involved in host\u2013pathogen interactions, metabolism, and signaling. Overall, this study emphasizes the need for molecular surveillance in regions where competent sandfly vectors are present.\n                  <\/jats:p>","DOI":"10.4269\/ajtmh.25-0744","type":"journal-article","created":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T15:22:44Z","timestamp":1776352964000},"page":"1104-1108","source":"Crossref","is-referenced-by-count":0,"title":["Genomic Characterization of a Locally Transmitted Leishmania mexicana Isolate from Texas"],"prefix":"10.4269","volume":"114","author":[{"given":"Juan David","family":"Ram\u00edrez","sequence":"first","affiliation":[{"name":"Center for Global Health and Inter-Disciplinary Research, USF Genomics Program, College of Public Health, University of South Florida, Tampa, Florida, USA;"},{"name":"School of Sciences and Engineering, Universidad del Rosario, Bogota, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luz H.","family":"Pati\u00f1o","sequence":"additional","affiliation":[{"name":"School of Sciences and Engineering, Universidad del Rosario, Bogota, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binita","family":"Nepal","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah M.","family":"Gunter","sequence":"additional","affiliation":[{"name":"Division of Tropical Medicine, Department of Pediatrics, National School of Tropical Medicine, Baylor College of Medicine and Texas Children\u2019s Hospital, Houston, Texas, USA;"},{"name":"The William T. Shearer Center for Human Immunobiology, Texas Children\u2019s Hospital, Houston, Texas, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva H.","family":"Clark","sequence":"additional","affiliation":[{"name":"Division of Tropical Medicine, Department of Pediatrics, National School of Tropical Medicine, Baylor College of Medicine and Texas Children\u2019s Hospital, Houston, Texas, USA;"},{"name":"Section of Infectious Diseases, Department of Medicine, Baylor College of Medicine, Houston, Texas, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dawn M.","family":"Wetzel","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, USA;"},{"name":"Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"issue":"3","key":"b1","series-title":"PLoS Negl Trop Dis","first-page":"e0004349","article-title":"A historical overview of the classification, evolution, and dispersion of Leishmania parasites and sandflies","volume":"10","author":"Akhoundi","year":"2016"},{"issue":"5","key":"b2","series-title":"PLoS One","first-page":"e35671","article-title":"Leishmaniasis worldwide and global estimates of its incidence","volume":"7","author":"Alvar","year":"2012"},{"key":"b3","article-title":"Leishmaniasis: Epidemiological Report for the Americas.","year":"2022"},{"issue":"4","key":"b4","series-title":"J Am Acad Dermatol","first-page":"650","article-title":"Cutaneous leishmaniasis in Texas: A northern spread of endemic areas","volume":"58","author":"Wright","year":"2008"},{"issue":"9","key":"b5","series-title":"JAMA Dermatol","first-page":"1032","article-title":"Incidence of endemic human cutaneous leishmaniasis in the United States","volume":"154","author":"McIlwee","year":"2018"},{"issue":"4","key":"b6","series-title":"Am J Trop Med Hyg","first-page":"1496","article-title":"An atypical case of autochthonous cutaneous leishmaniasis associated with naturally infected phlebotomine sand flies in Texas, United States","volume":"103","author":"Kipp","year":"2020"},{"issue":"8","key":"b7","series-title":"Biology (Basel)","first-page":"999","article-title":"Leishmania in Texas: A contemporary one health scoping review of vectors, reservoirs, and human health","volume":"14","author":"Jibowu","year":"2025"},{"issue":"3","key":"b8","series-title":"Microorganisms","first-page":"578","article-title":"Leishmaniasis in the United States: Emerging issues in a region of low endemicity","volume":"9","author":"Curtin","year":"2021"},{"issue":"3","key":"b9","series-title":"mSphere","first-page":"e0081423","article-title":"Cutaneous Leishmania mexicana infections in the United States: Defining strains through endemic human pediatric cases in northern 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