{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T13:20:46Z","timestamp":1788355246085,"version":"build-2803163510"},"reference-count":43,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,2]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n                  <jats:p>\n                    The impact of environmental contamination with\n                    <jats:italic>Echinococcus granulosus<\/jats:italic>\n                    eggs on the transmission to humans remains unknown. The control of free-roaming and stray dogs is a public health problem in urban and periurban areas of the Cusco region, such as the city of Sicuani in the Canchis province. Nearly one in four human cystic echinococcosis cases referred to tertiary hospitals in the Cusco region of Peru is referred from the Sicuani district. This study sought to determine the frequency and spatial distribution of\n                    <jats:italic>E. granulosus<\/jats:italic>\n                    DNA in dog stool collected within urban public spaces of Sicuani. We conducted a cross-sectional study using a systematic 100 \u00d7 100-meters\n                    <jats:sup>2<\/jats:sup>\n                    grid-based sampling design. Dog stools located at the closest point to georeferenced square vertices were selected for collection. Epidemiologically relevant locations, such as abattoirs and food markets, were also georeferenced. Nineteen (5.5%) of 347 collected samples tested positive for\n                    <jats:italic>E. granulosus<\/jats:italic>\n                    DNA. Polymerase chain reaction (PCR)-positive stools were located at a shorter median distance than negative stools to abattoirs (564 versus 1,292 meters, respectively,\n                    <jats:italic>P<\/jats:italic>\n                    &lt;0.01). Northern Sicuani, an area where informal slaughtering is performed, was identified as a hot spot for PCR-positive stools. The sequence analysis of the PCR products of positive samples closely aligned with sequences of\n                    <jats:italic>E. granulosus sensu stricto<\/jats:italic>\n                    (G1) with close phylogenetic relationship. Our field study revealed that\n                    <jats:italic>E. granulosus<\/jats:italic>\n                    PCR-positive dog stools were associated with spatial proximity to abattoirs. Interventions to decrease\n                    <jats:italic>E. granulosus<\/jats:italic>\n                    infections among dogs, particularly around abattoirs, may decrease the potential environmental contamination with infective stages.\n                  <\/jats:p>","DOI":"10.4269\/ajtmh.26-0009","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T15:24:17Z","timestamp":1784215457000},"page":"563-570","source":"Crossref","is-referenced-by-count":0,"title":["Frequency, Geospatial Distribution, and Subspecies of Echinococcus granulosus Identified in Dog Stool in Urban Public Spaces of an Endemic Area in Cusco, Peru"],"prefix":"10.4269","volume":"115","author":[{"given":"Miguel M.","family":"Cabada","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, University of Texas Medical Branch, Galveston, Texas, USA;"},{"name":"Universidad Peruana Cayetano Heredia and University of Texas Medical Branch Collaborative Research Center, Cusco, Peru;"},{"name":"Cusco Branch\u2013Alexander von Humboldt Tropical Medicine Institute\/Universidad Peruana Cayetano Heredia, Cusco, Peru;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Pineda-Reyes","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute Associated to University of Basel, Allschwil, Switzerland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nelson","family":"Lozano-Cansaya","sequence":"additional","affiliation":[{"name":"Cusco Branch\u2013Alexander von Humboldt Tropical Medicine Institute\/Universidad Peruana Cayetano Heredia, Cusco, Peru;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beltran","family":"Jalanocca-Janampa","sequence":"additional","affiliation":[{"name":"Cusco Branch\u2013Alexander von Humboldt Tropical Medicine Institute\/Universidad Peruana Cayetano Heredia, Cusco, Peru;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha V.","family":"Fernandez-Baca","sequence":"additional","affiliation":[{"name":"Universidad Peruana Cayetano Heredia and University of Texas Medical Branch Collaborative Research Center, Cusco, Peru;"},{"name":"Cusco Branch\u2013Alexander von Humboldt Tropical Medicine Institute\/Universidad Peruana Cayetano Heredia, Cusco, Peru;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carol A.","family":"Castro","sequence":"additional","affiliation":[{"name":"Universidad Peruana Cayetano Heredia and University of Texas Medical Branch Collaborative Research Center, Cusco, Peru;"},{"name":"Cusco Branch\u2013Alexander von Humboldt Tropical Medicine Institute\/Universidad Peruana Cayetano Heredia, Cusco, Peru;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Castellanos-Gonzalez","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, University of Texas Medical Branch, Galveston, Texas, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alonso","family":"Bussalleu","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute Associated to University of Basel, Allschwil, Switzerland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melinda B.","family":"Tanabe","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, University of Texas Medical Branch, Galveston, Texas, USA;"},{"name":"Universidad Peruana Cayetano Heredia and University of Texas Medical Branch Collaborative Research Center, Cusco, Peru;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey","family":"Schulze","sequence":"additional","affiliation":[{"name":"School of Medicine, University of Texas Medical Branch, Galveston, Texas, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Alvarez","sequence":"additional","affiliation":[{"name":"School of Medicine, University of Texas Medical Branch, Galveston, Texas, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria L.","family":"Morales","sequence":"additional","affiliation":[{"name":"Universidad Peruana Cayetano Heredia and University of Texas Medical Branch Collaborative Research Center, Cusco, Peru;"},{"name":"Cusco Branch\u2013Alexander von Humboldt Tropical Medicine Institute\/Universidad Peruana Cayetano Heredia, Cusco, Peru;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abigail","family":"Hitchcock","sequence":"additional","affiliation":[{"name":"Cusco Branch\u2013Alexander von Humboldt Tropical Medicine Institute\/Universidad Peruana Cayetano Heredia, Cusco, Peru;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruben","family":"Bascope","sequence":"additional","affiliation":[{"name":"Zoonotic Diseases Control Program, Cusco Regional Health Directorate, Peruvian Ministry of Health, Cusco, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakob","family":"Zinsstag","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute Associated to University of Basel, Allschwil, Switzerland;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"issue":"3","key":"b1","series-title":"J Clin Microbiol","first-page":"518","article-title":"Cystic echinococcosis","volume":"54","author":"Agudelo Higuita","year":"2016"},{"issue":"6","key":"b2","series-title":"Lancet Infect Dis","first-page":"385","article-title":"Prevention and control of cystic 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Vectors","first-page":"359","article-title":"Environmental contamination with feces of free-roaming dogs and the risk of transmission of Echinococcus and Taenia species in urban regions of southeastern Iran","volume":"17","author":"Shamsaddini","year":"2024"},{"issue":"1","key":"b12","series-title":"Parasitology","first-page":"84","article-title":"Environmental distribution of Echinococcus- and Taenia spp.-contaminated dog feces in Kyrgyzstan","volume":"151","author":"Abdykerimov","year":"2024"},{"issue":"12","key":"b13","series-title":"Vet Sci","first-page":"679","article-title":"Detection of Echinococcus granulosus sensu lato in environmental samples from Ibadan, Oyo State, South West Nigeria","volume":"9","author":"Awosanya","year":"2022"},{"issue":"1","key":"b14","series-title":"Korean J Parasitol","first-page":"113","article-title":"Environmental contamination by Echinococcus granulosus sensu lato eggs in relation to slaughterhouses in urban and rural areas in 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stray dogs in the Historic Center in the City of Cusco, Peru","volume":"2","author":"Romero Quispe","year":"2023"},{"key":"b19","article-title":"Compendio Estadistico, Cusco 2023.","year":"2023"},{"key":"b20","article-title":"City Population\u2014Sicuani, District in Cusco Region.","author":"Brinkhoff","year":"2026"},{"key":"b21","series-title":"Front Vet Sci","first-page":"617900","article-title":"Comparative study of free-roaming domestic dog management and roaming behavior across four countries: Chad, Guatemala, Indonesia, and Uganda","volume":"8","author":"Warembourg","year":"2021"},{"issue":"1","key":"b22","series-title":"Ecol Evol","first-page":"e8498","article-title":"Ranging patterns and factors associated with movement in free-roaming domestic dogs in urban Malawi","volume":"12","author":"De la Puente-Ar\u00e9valo","year":"2022"},{"issue":"3","key":"b23","series-title":"Am J Trop Med Hyg","first-page":"324","article-title":"Copro-diagnosis of Echinococcus granulosus infection 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