{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T16:52:43Z","timestamp":1782924763044,"version":"3.54.5"},"reference-count":18,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,1]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n                  <jats:p>\n                    In nonendemic countries such as Denmark, hookworm infections in travelers may be rare and difficult to detect because of low-grade shedding of parasite eggs and a lack of clinical suspicion and relevant diagnostic capacity. Two cases of hookworm infection observed in 2025 that were detected only through amplicon-based next-generation sequencing (NGS) of fecal DNA are presented in the current study. Both infections were characterized by hypereosinophilia and acquired during 2-week holidays in Asia. Case 1 was infected with\n                    <jats:italic>Ancylostoma ceylanicum<\/jats:italic>\n                    during a holiday in the Philippines, and Case 2 acquired infection with\n                    <jats:italic>Ancylostoma duodenale\/caninum<\/jats:italic>\n                    in Thailand. Eggs were found only in Case 2, and only after careful reexamination. These cases reveal that amplicon-based NGS is a simple and useful diagnostic adjunct when routine parasite screening triggered by eosinophilia and abdominal pain after tropical exposure fails to establish the diagnosis. Both cases were treated with ivermectin and albendazole, respectively, with good effect.\n                  <\/jats:p>","DOI":"10.4269\/ajtmh.26-0058","type":"journal-article","created":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T13:21:21Z","timestamp":1778160081000},"page":"77-80","source":"Crossref","is-referenced-by-count":0,"title":["Travelers Returning to Denmark with Hypereosinophilia and Ancylostoma Infections Detected Using Broad-Specificity Amplicon-Based Next-Generation Sequencing"],"prefix":"10.4269","volume":"115","author":[{"given":"Christen Rune","family":"Stensvold","sequence":"first","affiliation":[{"name":"Laboratory of Parasitology, Department of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen, Denmark;"},{"name":"Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriella","family":"Fredman","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Rigshospitalet, Copenhagen, Denmark;"},{"name":"Department of Dermatology, Copenhagen University Hospital Bispebjerg and Frederiksberg, Copenhagen, Denmark;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"Porskrog","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Zealand University Hospital, Roskilde, Roskilde, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Helleberg","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Rigshospitalet, Copenhagen, Denmark;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Nat Rev Dis Primers","first-page":"16088","article-title":"Hookworm infection","volume":"2","author":"Loukas","year":"2016"},{"issue":"10","key":"b2","series-title":"Emerg Infect Dis","first-page":"1936","article-title":"Plasmodium cynomolgi as cause of malaria in tourist to Southeast Asia, 2018","volume":"25","author":"Hartmeyer","year":"2019"},{"issue":"9","key":"b3","series-title":"BMJ Case Rep","first-page":"e247032","article-title":"Strongyloides stercoralis hyperinfection syndrome with cerebral involvement","volume":"15","author":"Pedersen","year":"2022"},{"key":"b4","series-title":"Travel Med Infect Dis","first-page":"102580","article-title":"First molecular documented case of a rarely reported parasite: Plasmodium knowlesi infection in Denmark in a traveller returning from Malaysian Borneo","volume":"53","author":"Aftab","year":"2023"},{"issue":"4","key":"b5","series-title":"Am J Trop Med Hyg","first-page":"685","article-title":"Simultaneous detection and quantification of Ancylostoma duodenale, Necator americanus, and Oesophagostomum bifurcum in fecal samples using multiplex real-time PCR","volume":"77","author":"Verweij","year":"2007"},{"key":"b6","series-title":"Methods Mol Biol","first-page":"115","article-title":"The Staden package, 1998","volume":"132","author":"Staden","year":"2000"},{"issue":"2","key":"b7","series-title":"J Infect","first-page":"106328","article-title":"UK guidelines for the investigation and management of eosinophilia in returning travellers and migrants","volume":"90","author":"Thakker","year":"2025"},{"issue":"13-14","key":"b8","series-title":"Int J Parasitol","first-page":"1233","article-title":"Zoonotic hookworms of dogs and cats - lessons from the past to inform current knowledge and future directions of research","volume":"51","author":"Traub","year":"2021"},{"issue":"3","key":"b9","series-title":"Emerg Infect Dis","first-page":"617","article-title":"Neurosarcocystosis in patient with HIV-induced immunodeficiency","volume":"31","author":"Skarpengland","year":"2025"},{"key":"b1","series-title":"Nat Rev Dis Primers","first-page":"16088","article-title":"Hookworm infection","volume":"2","author":"Loukas","year":"2016"},{"issue":"10","key":"b2","series-title":"Emerg Infect Dis","first-page":"1936","article-title":"Plasmodium cynomolgi as cause of malaria in tourist to Southeast Asia, 2018","volume":"25","author":"Hartmeyer","year":"2019"},{"issue":"9","key":"b3","series-title":"BMJ Case Rep","first-page":"e247032","article-title":"Strongyloides stercoralis hyperinfection syndrome with cerebral involvement","volume":"15","author":"Pedersen","year":"2022"},{"key":"b4","series-title":"Travel Med Infect Dis","first-page":"102580","article-title":"First molecular documented case of a rarely reported parasite: Plasmodium knowlesi infection in Denmark in a traveller returning from Malaysian Borneo","volume":"53","author":"Aftab","year":"2023"},{"issue":"4","key":"b5","series-title":"Am J Trop Med Hyg","first-page":"685","article-title":"Simultaneous detection and quantification of Ancylostoma duodenale, Necator americanus, and Oesophagostomum bifurcum in fecal samples using multiplex real-time PCR","volume":"77","author":"Verweij","year":"2007"},{"key":"b6","series-title":"Methods Mol Biol","first-page":"115","article-title":"The Staden package, 1998","volume":"132","author":"Staden","year":"2000"},{"issue":"2","key":"b7","series-title":"J Infect","first-page":"106328","article-title":"UK guidelines for the investigation and management of eosinophilia in returning travellers and migrants","volume":"90","author":"Thakker","year":"2025"},{"issue":"13-14","key":"b8","series-title":"Int J Parasitol","first-page":"1233","article-title":"Zoonotic hookworms of dogs and cats - 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