{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T14:57:17Z","timestamp":1791385037445,"version":"4.3.3"},"reference-count":59,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"4","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,7]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n                  <jats:p>\n                    The clinical and parasitological factors underlying severe malaria pathogenesis remain incompletely understood, particularly in\n                    <jats:italic>Plasmodium vivax<\/jats:italic>\n                    (\n                    <jats:italic>P. vivax<\/jats:italic>\n                    ). To address this issue, clinical and parasitological metrics, including circulating peripheral parasitemia and the parasite biomass (the total number of parasites in the body consisting of both circulating peripheral and noncirculating parasites), were measured in patients with severe and uncomplicated monoinfections of\n                    <jats:italic>P. vivax<\/jats:italic>\n                    and\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    (\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    ) malaria who presented at a hospital in southwest India. Peripheral parasitemia was positively correlated with disease severity in\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    but negatively correlated with disease severity in\n                    <jats:italic>P. vivax.<\/jats:italic>\n                    Contrarily, the parasite biomass, estimated by the levels of circulating antigens\n                    <jats:italic>P. vivax<\/jats:italic>\n                    lactate dehydrogenase and\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    histidine-rich protein 2 for\n                    <jats:italic>P. vivax<\/jats:italic>\n                    and\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    , respectively, was positively correlated with both the degree of severity and the length of hospital stay in both\n                    <jats:italic>P. vivax<\/jats:italic>\n                    and\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    cases. Together, these observations indicate a significant hidden parasite biomass in\n                    <jats:italic>P. vivax<\/jats:italic>\n                    infections and suggest that parasite biomass is a more robust parasitological marker of severity than parasitemia. Notably, thrombocytopenia was also positively correlated with parasite biomass and longer hospital stay in both\n                    <jats:italic>P. vivax<\/jats:italic>\n                    and\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    infections. These results highlight the importance of parasite biomass as a leading factor in the disease severity of malaria infections in India.\n                  <\/jats:p>","DOI":"10.4269\/ajtmh.26-0106","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T16:50:52Z","timestamp":1787849452000},"page":"795-806","source":"Crossref","is-referenced-by-count":0,"title":["Association of Parasite Biomass with Disease Severity in Plasmodium vivax and Plasmodium falciparum Malaria Infections"],"prefix":"10.4269","volume":"115","author":[{"given":"Prasad H.","family":"Babar","sequence":"first","affiliation":[{"name":"Department of Chemistry, University of Washington, Seattle, Washington, USA;"},{"name":"Department of Medicine, Goa Medical College and Hospital, Bambolim, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob A.","family":"Tennessen","sequence":"additional","affiliation":[{"name":"Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rimi","family":"Chakrabarti","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Washington, Seattle, Washington, USA;"},{"name":"Department of Medicine, Goa Medical College and Hospital, Bambolim, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jayashri T.","family":"Walke","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Washington, Seattle, Washington, USA;"},{"name":"Department of Medicine, Goa Medical College and Hospital, Bambolim, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Usheer","family":"Kanjee","sequence":"additional","affiliation":[{"name":"Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anvily","family":"Almeida","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Washington, Seattle, Washington, USA;"},{"name":"Department of Medicine, Goa Medical College and Hospital, Bambolim, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mezia","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Washington, Seattle, Washington, USA;"},{"name":"Department of Medicine, Goa Medical College and Hospital, Bambolim, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pooja","family":"Gawas","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Washington, Seattle, Washington, USA;"},{"name":"Department of Medicine, Goa Medical College and Hospital, Bambolim, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Karschney","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Washington, Seattle, Washington, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"White","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Washington, Seattle, Washington, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Vaz","sequence":"additional","affiliation":[{"name":"Department of Medicine, Goa Medical College and Hospital, Bambolim, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwin","family":"Gomes","sequence":"additional","affiliation":[{"name":"Department of Medicine, Goa Medical College and Hospital, Bambolim, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pradipsinh K.","family":"Rathod","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Washington, Seattle, Washington, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manoj T.","family":"Duraisingh","sequence":"additional","affiliation":[{"name":"Department of Immunology and Infectious Diseases, Harvard T.H. 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