{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T14:58:01Z","timestamp":1791385081968,"version":"4.3.3"},"reference-count":10,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,7]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n                  <jats:p>\n                    Accurate identification of\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    species is essential for appropriate malaria management, particularly in cases of\n                    <jats:italic>Plasmodium vivax<\/jats:italic>\n                    (\n                    <jats:italic>P. vivax<\/jats:italic>\n                    ), in which relapse prevention requires radical cure. A diagnostically challenging case of late-onset malaria in a Swedish woman presenting with tertian fever 5 months after travel to Indonesia is described. Although loop-mediated isothermal amplification repeatedly indicated\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    infection, routine microscopy and multiple independent species-specific quantitative polymerase chain reaction (qPCR) assays failed to identify the infecting species. Conventional polymerase chain reaction and Sanger sequencing confirmed the presence of\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    DNA but did not enable reliable species determination. Amplicon-based next-generation sequencing (NGS) targeting the small subunit ribosomal RNA (rRNA) gene generated more than 5,000\n                    <jats:italic>P. vivax<\/jats:italic>\n                    reads. Sequence polymorphisms in primer\/probe binding regions explained the failure of standard qPCR assays. After species identification and confirmation of normal G6PD activity, the patient received primaquine for hypnozoite eradication. This case highlights the diagnostic value of NGS in malaria and the limitations of relying solely on conserved 18S rDNA targets.\n                  <\/jats:p>","DOI":"10.4269\/ajtmh.26-0148","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T14:29:35Z","timestamp":1785853775000},"page":"762-765","source":"Crossref","is-referenced-by-count":0,"title":["A Plasmodium vivax Case Identified Solely via the Use of Next-Generation Sequencing"],"prefix":"10.4269","volume":"115","author":[{"given":"Christen Rune","family":"Stensvold","sequence":"first","affiliation":[{"name":"Laboratory of Parasitology, Department of Bacteriology, Parasitology, and Mycology, Statens Serum Institut, Copenhagen, Denmark;"},{"name":"Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik Vedel","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology, Department of Bacteriology, Parasitology, and Mycology, Statens Serum Institut, Copenhagen, Denmark;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John Kerr","family":"White","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden;"},{"name":"Department of Microbiology, Tumor and Cell Biology Karolinska Institutet, Stockholm, Sweden;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Szeps","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Karolinska University Hospital, Stockholm, Sweden;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katja","family":"Wyss","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Karolinska University Hospital, Stockholm, Sweden;"},{"name":"Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Botero-Kleiven","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"issue":"4","key":"b1","series-title":"J Clin Microbiol","first-page":"975","article-title":"Multiplexed real-time PCR assay for discrimination of Plasmodium species with improved sensitivity for mixed infections","volume":"47","author":"Shokoples","year":"2009"},{"key":"b2","series-title":"Malar J","first-page":"344","article-title":"A TaqMan real-time PCR assay for the detection and quantitation of Plasmodium knowlesi","volume":"9","author":"Divis","year":"2010"},{"issue":"10","key":"b3","series-title":"Emerg Infect Dis","first-page":"1936","article-title":"Plasmodium cynomolgi as 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Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/115\/4\/article-p762.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/115\/4\/article-p762.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/115\/4\/article-p762.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T14:24:44Z","timestamp":1791383084000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/115\/4\/article-p762.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,7]]},"references-count":10,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.26-0148","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,7]]}}}