{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T15:39:38Z","timestamp":1792510778869,"version":"4.5.0"},"reference-count":20,"publisher":"American Society of Tropical Medicine and Hygiene","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,20]]},"abstract":"ABSTRACT.<\/jats:title>\n \n Tanzania recently identified partial artemisinin resistance in the Kagera region bordering Rwanda and Uganda, where resistance is established. Monitoring neighboring regions is critical to guide containment strategies. We, therefore, conducted molecular surveillance of\n Plasmodium falciparum Kelch13<\/jats:italic>\n (\n pfk13<\/jats:italic>\n ) and\n Plasmodium falciparum actin-binding protein coronin<\/jats:italic>\n (\n pfcoronin<\/jats:italic>\n ; P76S) mutations in Igombe in Mwanza region. In a health facility-based cross-sectional study in 2025,\n P. falciparum<\/jats:italic>\n DNA from patients with uncomplicated malaria was analyzed using polymerase chain reaction, Sanger sequencing, and allele-specific melting curve assays. Among 121 sequenced isolates, no validated or candidate\n pfk13<\/jats:italic>\n mutations were detected. The nonsynonymous, nonvalidated\n pfk13<\/jats:italic>\n variant N489K occurred in 2.5%. The\n pfcoronin<\/jats:italic>\n P76S mutation was observed at low prevalence (3.6%). Despite proximity to Kagera hot spots, validated\n pfk13<\/jats:italic>\n markers have not yet been established in the Mwanza region. The presence of\n pfcoronin<\/jats:italic>\n P76S, albeit at low levels, requires careful monitoring. Continued molecular surveillance is essential to support the early detection and containment of emerging artemisinin resistance in Tanzania.\n <\/jats:p>","DOI":"10.4269\/ajtmh.26-0347","type":"journal-article","created":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T14:50:00Z","timestamp":1792507800000},"source":"Crossref","is-referenced-by-count":0,"title":["Absence of Validated and Candidate Plasmodium falciparum Kelch13 Markers of Artemisinin Resistance and Low Frequency of a Pfcoronin Mutation in Igombe, Mwanza Region, Tanzania"],"prefix":"10.4269","author":[{"given":"Karol J.","family":"Marwa","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Catholic University of Health and Allied Sciences, Mwanza, Tanzania;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Institute of International Health, Charit\u00e9 Center for Global Health, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Berlin, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard O.","family":"Mwaiswelo","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Parasitology, Kairuki University, Dar es Salaam, Tanzania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Welmoed","family":"van Loon","sequence":"additional","affiliation":[{"name":"Institute of International Health, Charit\u00e9 Center for Global Health, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Berlin, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank P.","family":"Mockenhaupt","sequence":"additional","affiliation":[{"name":"Institute of International Health, Charit\u00e9 Center for Global Health, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Berlin, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"issue":"1","key":"b1","series-title":"J Infect Dis","first-page":"269","article-title":"High frequency of artemisinin partial resistance mutations in the Great Lakes Region revealed through rapid pooled deep sequencing","volume":"231","author":"Wernsman Young","year":"2025"},{"issue":"8","key":"b2","series-title":"N Engl J Med","first-page":"722","article-title":"Evolution of partial resistance to artemisinins in malaria parasites in Uganda","volume":"389","author":"Conrad","year":"2023"},{"key":"b3","article-title":"Mapping the Prevalence of Molecular Markers of Plasmodium falciparum Artemisinin Partial Resistance in Africa: A Spatial-Temporal Modelling Study.","author":"Young","year":"2025"},{"issue":"10","key":"b4","series-title":"Lancet Microbe","first-page":"100920","article-title":"Prevalence of mutations associated with artemisinin partial resistance and sulfadoxine-pyrimethamine resistance in 13 regions in Tanzania in 2021: A cross-sectional survey","volume":"5","author":"Juliano","year":"2024"},{"key":"b5","series-title":"Front Genet","first-page":"1776108","article-title":"Temporal trends in artemisinin partial resistance and other antimalarial drug mutations in Plasmodium falciparum from Kagera region, northwestern Tanzania, 2021\u20132023","volume":"17","author":"Simkin","year":"2026"},{"issue":"50","key":"b6","series-title":"Proc Natl Acad Sci USA","first-page":"12799","article-title":"Mutations in Plasmodium falciparum actin-binding protein coronin confer reduced artemisinin susceptibility","volume":"115","author":"Demas","year":"2018"},{"issue":"1","key":"b7","series-title":"Malar J","first-page":"451","article-title":"Prevalence of potential mediators of artemisinin resistance in African isolates of Plasmodium falciparum.","volume":"20","author":"Owoloye","year":"2021"},{"issue":"8","key":"b8","series-title":"J Travel Med","first-page":"taad114","article-title":"Emergence of artemisinin-based combination treatment failure in patients returning from sub-Saharan Africa with P. falciparum malaria","volume":"30","author":"Grossman","year":"2023"},{"issue":"1","key":"b9","series-title":"J Infect Dis","first-page":"251","article-title":"Efficacy and safety of artemether-lumefantrine against uncomplicated falciparum malaria infection in Tanzania, 2022: A single-arm clinical trial","volume":"231","author":"Laury","year":"2025"},{"issue":"1","key":"b10","series-title":"Malar J","first-page":"71","article-title":"Trends of Plasmodium falciparum molecular markers associated with resistance to artemisinins and reduced susceptibility to lumefantrine in Mainland Tanzania from 2016 to 2021","volume":"23","author":"Bakari","year":"2024"},{"issue":"7481","key":"b11","series-title":"Nature","first-page":"50","article-title":"A molecular marker of artemisinin-resistant Plasmodium falciparum malaria","volume":"505","author":"Ariey","year":"2014"},{"issue":"1","key":"b12","series-title":"Trop Med Int Health","first-page":"43","article-title":"Molecular markers of antimalarial drug resistance in Plasmodium falciparum from Kumasi, Ghana, 2023","volume":"31","author":"Kegya","year":"2026"},{"issue":"5","key":"b13","series-title":"Lancet Microbe","first-page":"101027","article-title":"Selection of Plasmodium falciparum kelch13 mutations in Uganda in comparison with southeast Asia: A modelling study","volume":"6","author":"Meier-Scherling","year":"2025"},{"issue":"10","key":"b14","series-title":"Nat Med","first-page":"1602","article-title":"Emergence and clonal expansion of in\u00a0vitro artemisinin-resistant Plasmodium falciparum kelch13 R561H mutant parasites in Rwanda","volume":"26","author":"Uwimana","year":"2020"},{"issue":"8","key":"b15","series-title":"Lancet Infect Dis","first-page":"1120","article-title":"Association of Plasmodium falciparum kelch13 R561H genotypes with delayed parasite clearance in Rwanda: An open-label, single-arm, multicentre, therapeutic efficacy study","volume":"21","author":"Uwimana","year":"2021"},{"issue":"1","key":"b16","series-title":"Antimicrob Agents Chemother","first-page":"e0129923","article-title":"Escalating Plasmodium falciparum K13 marker prevalence indicative of artemisinin resistance in southern Rwanda","volume":"68","author":"van Loon","year":"2024"},{"issue":"4","key":"b17","series-title":"Am J Trop Med Hyg","first-page":"653","article-title":"Detection of artemisinin resistance marker Kelch-13 469Y in Plasmodium falciparum, South Kivu, Democratic Republic of the Congo, 2022","volume":"110","author":"van Loon","year":"2024"},{"issue":"4","key":"b18","series-title":"Emerg Infect Dis","first-page":"553","article-title":"Border region surveillance of malaria drug resistance, northern Burundi, 2023\u20132024","volume":"32","author":"Niyomwungere","year":"2026"},{"key":"b19","doi-asserted-by":"crossref","DOI":"10.21203\/rs.3.rs-6214166\/v1","article-title":"Emergence and Spread of Plasmodium falciparum Kelch 13 Mutations in Selected Counties of Kenya: Implications for Responding to Artemisinin Partial Resistance.","author":"Akala","year":"2025"},{"issue":"12","key":"b20","series-title":"PLoS Genet","first-page":"e1009266","article-title":"Genetic background and PfKelch13 affect artemisinin susceptibility of PfCoronin mutants in Plasmodium falciparum.","volume":"16","author":"Sharma","year":"2020"}],"container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/aop\/article-10.4269-ajtmh.26-0347\/article-10.4269-ajtmh.26-0347.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/aop\/article-10.4269-ajtmh.26-0347\/article-10.4269-ajtmh.26-0347.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T14:50:04Z","timestamp":1792507804000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/aop\/article-10.4269-ajtmh.26-0347\/article-10.4269-ajtmh.26-0347.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,20]]},"references-count":20,"URL":"https:\/\/doi.org\/10.4269\/ajtmh.26-0347","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,20]]}}}