{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:03:44Z","timestamp":1760123024414},"reference-count":0,"publisher":"African Journals Online (AJOL)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Afr H. Sci."],"abstract":"<jats:p>Background: Escherichia coli is the most predominant pathogen involved in UTIs. Mainly, fimbrial surface appendages are impli- cated in adherence to urothelium besides non-fimbrial proteins.&#x0D;\nObjectives: To determine prevalence of genes encoding fimbrial and non-fimbrial proteins among Uropathogenic Escherichia coli (UPEC). Furthermore, distribution of these genes and biofilm formation capacity were investigated in relation to antimicrobial resistance.&#x0D;\nMethods: Antimicrobial susceptibility of 112 UPEC isolates was performed using disc diffusion method. ESBL production was confirmed by double disc synergy test. Genes encoding fimbrial and non-fimbrial proteins were detected using PCR and biofilm formation was investigated using microtitre plate assay.&#x0D;\nResults: UPEC isolates exhibited high resistance against doxycyclines (88.39 %), \u03b2-lactams (7.14-86.6%), sulphamethoxaz- ole\u2013trimethoprim (53.75%) and fluoro-quinolones (50%). Fifty percent of tested isolates were ESBL producers. PapGII gene was statistically more prevalent among pyelonephritis isolates. SfaS, focG and picU genes were statistically associated with flu- oro-quinolone (FQs) sensitive isolates and Dr\/afaBC gene was statistically associated with ESBL production. Moreover, non- MDR isolates produced sturdier biofilm.&#x0D;\nConclusion: PapGII adhesin variant seems to have a critical role in colonization of upper urinary tract. There is a possible link between antimicrobial resistance and virulence being capable of affecting the distribution of some genes besides its negative impact on biofilm formation.&#x0D;\nKeywords: Urinary tract infection; Escherichia coli; UPEC; adhesin genes; ESBL; biofilm.\u00a0\u00a0<\/jats:p>","DOI":"10.4314\/ahs.v20i1.29","type":"journal-article","created":{"date-parts":[[2020,10,8]],"date-time":"2020-10-08T11:05:22Z","timestamp":1602155122000},"page":"238-247","source":"Crossref","is-referenced-by-count":9,"title":["Distribution of genes encoding adhesins and biofilm formation capacity among Uropathogenic Escherichia coli isolates in relation to the antimicrobial resistance"],"prefix":"10.4314","volume":"20","author":[{"given":"Ashraf","family":"A Kadry","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nour","family":"M Al-Kashef","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amira","family":"M El-Ganiny","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2895","published-online":{"date-parts":[[2020,4,20]]},"container-title":["African Health Sciences"],"original-title":[],"link":[{"URL":"https:\/\/www.ajol.info\/index.php\/ahs\/article\/download\/195012\/184190","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajol.info\/index.php\/ahs\/article\/download\/195012\/184190","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,8]],"date-time":"2020-10-08T11:06:09Z","timestamp":1602155169000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajol.info\/index.php\/ahs\/article\/view\/195012"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,20]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,4,23]]}},"URL":"https:\/\/doi.org\/10.4314\/ahs.v20i1.29","relation":{},"ISSN":["1680-6905"],"issn-type":[{"value":"1680-6905","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,20]]}}}