{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T11:02:07Z","timestamp":1761562927485},"reference-count":0,"publisher":"African Journals Online (AJOL)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Afr H. Sci."],"abstract":"<jats:p>Background: Cerebral ischemia reperfusion injury (CIRI) is the pathophysiological basis of various cerebrovascular diseases. The aim of this study was to explore the role of HIF-1\u03b1\/BNIP3 in the alleviating effect of IL-4 on CIRI in mice.&#x0D;\nMethodology: Mice were randomly divided into sham operation (Sham), ischemia reperfusion (IR), IL-4, HIF-1\u03b1 inhibitor 2ME2 and IL-4+2ME2 groups. Middle cerebral artery occlusion model was established. After 24-h reperfusion, neurologic deficit score (NDS) was given. Cerebral infarction volume and brain water content were measured by 2,3,5-triphenyltetrazolium chloride staining and dry-wet weights, respectively. Apoptosis was detected by TUNEL staining. SOD, MDA and ROS levels, and HIF-1\u03b1, BNIP3, LC3II and Beclin-1 expressions were detected through colorimetry and Western blotting, respectively.&#x0D;\nResults: Compared with IR group, NDS, cerebral infarction volume, brain water content, apoptosis rate, and MDA and ROS levels decreased, while SOD, HIF-1\u03b1, BNIP3, LC3-II and Beclin-1 levels increased in IL-4 group (P&lt;0.05). 2ME2 and IL-4+2ME2 groups had decreased NDS, cerebral infarction volume, brain water content, apoptosis rate and MDA, ROS, HIF-1\u03b1, BNIP3, LC3-II and Beclin-1 levels, but increased SOD level compared with those of IL-4 group (P&lt;0.05).&#x0D;\nConclusion: IL-4 reduces apoptosis and oxidative stress through activating the HIF-1\u03b1\/BNIP3 pathway, thereby alleviating mouse CIRI.&#x0D;\nKeywords: Hypoxia inducible factor 1\u03b1; Bcl-2\/adenovirus E1B 19-kDa interacting protein 3; interleukin-4; cerebral ischemia reperfusion injury.<\/jats:p>","DOI":"10.4314\/ahs.v22i3.39","type":"journal-article","created":{"date-parts":[[2022,11,15]],"date-time":"2022-11-15T13:21:53Z","timestamp":1668518513000},"page":"369-374","source":"Crossref","is-referenced-by-count":2,"title":["Role of hypoxia inducible factor 1\u03b1\/Bcl-2\/adenovirus E1B 19-kDa interacting protein 3 in alleviating effect of interleukin-4 on cerebral ischemia reperfusion injury in mice"],"prefix":"10.4314","volume":"22","author":[{"given":"Tianjing","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2895","published-online":{"date-parts":[[2022,10,28]]},"container-title":["African Health Sciences"],"original-title":[],"link":[{"URL":"https:\/\/www.ajol.info\/index.php\/ahs\/article\/download\/234929\/221932","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajol.info\/index.php\/ahs\/article\/download\/234929\/221932","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,15]],"date-time":"2022-11-15T13:23:06Z","timestamp":1668518586000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajol.info\/index.php\/ahs\/article\/view\/234929"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,28]]},"references-count":0,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2022,10,28]]}},"URL":"https:\/\/doi.org\/10.4314\/ahs.v22i3.39","relation":{},"ISSN":["1729-0503","1680-6905"],"issn-type":[{"value":"1729-0503","type":"electronic"},{"value":"1680-6905","type":"print"}],"subject":[],"published":{"date-parts":[[2022,10,28]]}}}