{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T14:58:39Z","timestamp":1789570719313,"version":"build-2803163510"},"reference-count":0,"publisher":"African Journals Online (AJOL)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Afr H. Sci."],"abstract":"<jats:p>Background: Successful elimination of hepatitis as a public health threat by 2030 will partly rely on the availability and accessibility of affordable accurate disease testing platforms. In the past, testing of hepatitis C virus (HCV) in low resource settings of sub-Saharan Africa (SSA) has relied on anti-HCV testing using rapid diagnostic tests, chemiluminescent microparticle immunoassay (CMIA) and Enzyme-linked Immunosorbent Assays (ELISA) whose diagnostic accuracy has been sub-optimal. We determined the false positivity rate of a CMIA platform that is routinely used to screen donor blood for anti-HCV in Uganda.\r\nMethods: 1,216 CMIA-screened anti-HCV-positive blood donor samples at four regional Ugandan blood banks, were subjected to a third generation ELISA and subsequently to nucleic acid testing (NAT).\r\nResults: Of the above 1,216 samples, 1,122 (92.2%) were negative on ELISA and thus deemed false positives. Active infection (NAT positive) was detected in 98 (8.0%). Presumed resolved infection was recorded among 3 (3.2%) of participants that remained positive on the ELISA platform but negative on NAT.\r\nConclusion: The Architect CMIA assay exhibited very low specificity for anti-HCV testing. In this context, this finding may suggest need to employ testing protocols that include NAT or a combination of tests with higher validity.\r\nKeywords: High false hepatitis C; antibody positivity rate; non-renumerated blood donors; Uganda.<\/jats:p>","DOI":"10.4314\/ahs.v24i3.6","type":"journal-article","created":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T12:54:09Z","timestamp":1728996849000},"page":"41-46","source":"Crossref","is-referenced-by-count":0,"title":["High false hepatitis C antibody positivity rate in a regionally-inclusive  population of non-renumerated blood donors in Uganda"],"prefix":"10.4314","volume":"24","author":[{"given":"P","family":"Ocama","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Ssekitoleko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Nankya-Mutyoba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"Apica","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Otekat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Seremba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2895","published-online":{"date-parts":[[2024,10,6]]},"container-title":["African Health Sciences"],"original-title":[],"link":[{"URL":"https:\/\/www.ajol.info\/index.php\/ahs\/article\/download\/280007\/264113","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajol.info\/index.php\/ahs\/article\/download\/280007\/264113","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T12:54:45Z","timestamp":1728996885000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajol.info\/index.php\/ahs\/article\/view\/280007"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,6]]},"references-count":0,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2024,10,6]]}},"URL":"https:\/\/doi.org\/10.4314\/ahs.v24i3.6","relation":{},"ISSN":["1729-0503","1680-6905"],"issn-type":[{"value":"1729-0503","type":"electronic"},{"value":"1680-6905","type":"print"}],"subject":[],"published":{"date-parts":[[2024,10,6]]}}}