{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,10,16]],"date-time":"2024-10-16T04:20:11Z","timestamp":1729052411246},"reference-count":0,"publisher":"African Journals Online (AJOL)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Afr H. Sci."],"abstract":"<jats:p>Background: The inappropriate use of antibiotics may cause antibiotic resistance, cause side effects, and eventually cause an increase in healthcare costs. This study aimed to determine a flow cytometry-based test to detect ESBL E. coli and MDR p. aeruginosa in a short time.\r\nMethods: The study included 25 E.coli isolates and 25 P.aeruginosa isolates identified by Phoenix TM 100 automated system [Becton Dickinson, USA]. In the flow cytometric method, the percentages of death cells exposed to cephalosporin including ceftazidime [CAZ] or cefotaxime [CTX] and clavulanic acid [CLA] combination, were compared with death cells exposed only to a cephalosporin [CAZ or CTX]. CLA index values [CAZ-CLA and CTX-CLA indices] were obtained for CTX and CAZ. Index values that were higher than 1.5 just for one cephalosporin were accepted as positive.\r\nResults: Cell viability was performed after 1-hour exposure to the drugs. When PI staining was applied to ESBL-treated and MDR-treated bacteria, 65% and 85% had nonviable membranes, indicating the method efficiently identified only cells with damaged membranes.\r\nConclusion: Flow cytometry is a rapid and reliable method for the detection of ESBL and MDR in clinical microbiology laboratories\r\nKeywords: Antibiotic resistance; ESBL; flow cytometry; MDR.<\/jats:p>","DOI":"10.4314\/ahs.v24i3.8","type":"journal-article","created":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T12:54:09Z","timestamp":1728996849000},"page":"58-62","source":"Crossref","is-referenced-by-count":0,"title":["Can flow cytometry be an alternative method for the detection  of antimicrobial resistance?"],"prefix":"10.4314","volume":"24","author":[{"given":"Ozel","family":"Yuruker","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emrah","family":"G\u00fcler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaya","family":"S\u00fcer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meryem","family":"G\u00fcvenir","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2895","published-online":{"date-parts":[[2024,10,6]]},"container-title":["African Health Sciences"],"original-title":[],"link":[{"URL":"https:\/\/www.ajol.info\/index.php\/ahs\/article\/download\/280010\/264115","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajol.info\/index.php\/ahs\/article\/download\/280010\/264115","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T12:54:44Z","timestamp":1728996884000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajol.info\/index.php\/ahs\/article\/view\/280010"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,6]]},"references-count":0,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2024,10,6]]}},"URL":"https:\/\/doi.org\/10.4314\/ahs.v24i3.8","relation":{},"ISSN":["1729-0503","1680-6905"],"issn-type":[{"value":"1729-0503","type":"electronic"},{"value":"1680-6905","type":"print"}],"subject":[],"published":{"date-parts":[[2024,10,6]]}}}