{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T08:04:14Z","timestamp":1784793854095,"version":"3.55.0"},"reference-count":0,"publisher":"African Journals Online (AJOL)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Afr H. Sci."],"abstract":"<jats:p>Background: Vancomycin-resistant Enterococcus faecium is classified among World Health Organization priority pathogens for Research and development of new antibiotics. This study presents the results of 13-year surveillance of antibiotic resistance in E. faecium, with a specific focus on vancomycin and linezolid resistance in thirteen university hospitals across various regions of Tunisia.\r\nMethods: All participating laboratories followed a standardized methodology for clinical sample culture, bacterial identification, antibiotic susceptibility testing according to CA-SFM guidelines, result interpretation, epidemiological data collection. Additionally, they implemented quality controls.\r\nFindings: During the surveillance period, 2,931 E. faecium isolates were collected, predominantly from urine (49.1%) and blood cultures (24%), with high prevalence in Surgery Departments (30.3%) and Intensive Care Units (23.6%). Resistance rates were high for ampicillin (87.1%), erythromycin (93.6%), gentamicin (62.8%) and vancomycin (26%). Since 2019, six linezolid-resistant isolates have been isolated and only one isolate resistant to tigecycline was identified in 2021. Vancomycin resistance had significant upward trend since 2012, peaking at 38.1% in 2017. It was significantly associated to gentamicin (84.9% vs. 53.8%) (p&lt;0.0001) and erythromycin95.8% vs. 89.6%) (p=0.0001) in a five year period (2018 - 2022).\r\nConclusion: This worrying situation requires deeper investigations to develop adapted strategies with targeted measures in high-risk departments.\r\nKeywords: Enterococcus faecium; vancomycin resistance; linezolid resistance.<\/jats:p>","DOI":"10.4314\/ahs.v26i2.5","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T07:28:33Z","timestamp":1784791713000},"page":"31-37","source":"Crossref","is-referenced-by-count":0,"title":["Enterococcus faecium multicenter antimicrobial resistance surveillance in Tunisia (2010-2022): high vancomycin resistance rates and linezolid resistance emergence"],"prefix":"10.4314","volume":"26","author":[{"given":"Anis","family":"Raddaoui","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mouna","family":"Louisa Ben 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