{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T22:55:00Z","timestamp":1722984900095},"reference-count":0,"publisher":"ScopeMed","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Open Vet J."],"abstract":"<jats:p>Background: American Foulbrood (AFB) is a devastating disease of honey bee (Apis mellifera) larvae caused by the spore-forming, Gram-positive bacterium Paenibacillus larvae. In most countries, the law requires mandatory reporting of AFB to the veterinary authority.Aim and Methods: To speed up detection and genotyping of P. larvae spores, we compared different culturing protocols on Columbia sheep blood agar and developed a new multiplex quantitative polymerase chain reaction to distinguish between the two relevant P. larvae genotypes enterobacterial repetitive intergenic consensus (ERIC) I and ERIC II.Results and Conclusion: As confirmed by P. larvae reference strains and field isolates, the new identification and genotyping protocol halves the time of current workflows, lessens labor-intension, allows a higher throughput of samples for monitoring, and permits a faster intervention to prevent the spread of AFB.&#x0D;\nKeywords: American Foulbrood, ERIC genotyping, Multiplex quantitative PCR, Paenibacillus larvae, Rapid detection.<\/jats:p>","DOI":"10.4314\/ovj.v10i1.9","type":"journal-article","created":{"date-parts":[[2020,6,25]],"date-time":"2020-06-25T12:03:23Z","timestamp":1593086603000},"source":"Crossref","is-referenced-by-count":10,"title":["Rapid identification and genotyping of the honeybee pathogen &lt;i&gt;Paenibacillus larvae&lt;\/i&gt; by combining culturing and multiplex quantitative PCR"],"prefix":"10.5455","volume":"10","author":[{"given":"Hannes","family":"Beims","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martina","family":"Janke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Werner","family":"Von der Ohe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Steinert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3407","published-online":{"date-parts":[[2020,4,21]]},"container-title":["Open Veterinary Journal"],"original-title":[],"link":[{"URL":"https:\/\/www.ajol.info\/index.php\/ovj\/article\/download\/194975\/184159","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajol.info\/index.php\/ovj\/article\/download\/194975\/184159","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,25]],"date-time":"2021-03-25T22:20:56Z","timestamp":1616710856000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajol.info\/index.php\/ovj\/article\/view\/194975"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,21]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,4,21]]}},"URL":"https:\/\/doi.org\/10.4314\/ovj.v10i1.9","relation":{},"ISSN":["2218-6050","2226-4485"],"issn-type":[{"value":"2218-6050","type":"electronic"},{"value":"2226-4485","type":"print"}],"subject":[],"published":{"date-parts":[[2020,4,21]]}}}