{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T11:10:09Z","timestamp":1749813009702,"version":"3.41.0"},"reference-count":0,"publisher":"African Journals Online (AJOL)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["UJAS"],"abstract":"<jats:p>This study aimed to develop a safe bio-control approach for therapy and decontamination of Aeromonas hydrophila. The study focused on determination of the drug susceptibility ofAeromonas isolates from diseased tilapia; and establishment of a stock of partially characterized Aeromonas hydrophila bacteriophages. Eighteen (18) Aeromonas spp isolates were tested for antimicrobial resistance against 10 antibiotics. The susceptibility of the isolates to various antibiotics ranged from 0 to 100%. For bacteriophages, three lytic phages that had the highest host range (78.6% to 92.8%, n=28) were selected. Further characterization revealed a burst period of 20 \u00b1 5 to 40 \u00b1 5 minutes, a latent period that varied from 10 \u00b1 5 to 40 \u00b1 5 minutes; and burst size of 98\u2013171 virions per infected cell. There were no significant changes in phage titers on exposure at 40\u2103 and 50\u2103 for 60 minutes (P = 0.05). However, a gradual decrease in the titres was observed at 60\u2103 while a fast decline was noted at 70\u2103-90\u2103. All phages demonstrated at least some activity in the pH range of 1-13. In water stability testing, the phages survived up to 22 days of the experiment, with a sharp decrease of 1 log PFU\/mL to have been encountered within the first day. The findings of this study suggest phages as bio-control agents against Aeromonas infection in fish. However, further genomic characterization is required.<\/jats:p>","DOI":"10.4314\/ujas.v22i2.3","type":"journal-article","created":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T10:43:36Z","timestamp":1749811416000},"page":"31-46","source":"Crossref","is-referenced-by-count":0,"title":["Isolation and Characterization of Aeromonas hydrophila lytic Bacteriophages as a safe antibiotic alternative bio-control agent"],"prefix":"10.4314","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8333-1099","authenticated-orcid":false,"given":"Raphael","family":"Hans Lwesya","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2562-718X","authenticated-orcid":false,"given":"Samuel","family":"P. Wamala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7208-7120","authenticated-orcid":false,"given":"Claire","family":"M. Mugasa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3509-9827","authenticated-orcid":false,"given":"John","family":"Walakira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4585-5163","authenticated-orcid":false,"given":"Jesca","family":"L. Nakavuma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2895","published-online":{"date-parts":[[2024,11,2]]},"container-title":["Uganda Journal of Agricultural Sciences"],"original-title":[],"link":[{"URL":"https:\/\/www.ajol.info\/index.php\/ujas\/article\/download\/282048\/265768","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajol.info\/index.php\/ujas\/article\/download\/282048\/265768","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T10:43:38Z","timestamp":1749811418000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajol.info\/index.php\/ujas\/article\/view\/282048"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,2]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2024,11,2]]}},"URL":"https:\/\/doi.org\/10.4314\/ujas.v22i2.3","relation":{},"ISSN":["2410-6909","1026-0919"],"issn-type":[{"value":"2410-6909","type":"electronic"},{"value":"1026-0919","type":"print"}],"subject":[],"published":{"date-parts":[[2024,11,2]]}}}