{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T13:51:14Z","timestamp":1787320274934,"version":"build-2736575974"},"reference-count":45,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"vor","delay-in-days":275,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Protection"],"published-print":{"date-parts":[[2022,3]]},"DOI":"10.4315\/jfp-21-354","type":"journal-article","created":{"date-parts":[[2021,11,17]],"date-time":"2021-11-17T16:03:55Z","timestamp":1637165035000},"page":"390-397","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"title":["High Pressure Thermal Sterilization and \u03b5-Polylysine Synergistically Inactivate Bacillus subtilis Spores by Damaging the Inner Membrane"],"prefix":"10.1016","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3441-727X","authenticated-orcid":false,"given":"Yue","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6787-6291","authenticated-orcid":false,"given":"Zhong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Bi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bianfei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weishan","family":"Xin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.4315\/JFP-21-354_bb0010","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.ijfoodmicro.2010.06.008","article-title":"Changes in Fourier transform infrared spectra of Salmonella enterica serovars Typhimurium and Enteritidis after adaptation to stressful growth conditions","volume":"142","author":"Alvarez-Ord\u00f3\u00f1ez,","year":"2010","journal-title":"Int. J. Food Microbiol"},{"key":"10.4315\/JFP-21-354_bb0015","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1016\/j.foodcont.2015.08.033","article-title":"Comparison of spore inactivation with novel agitating retort, static retort and combined high pressure-temperature treatments","volume":"60","author":"Ates,","year":"2016","journal-title":"Food Control"},{"key":"10.4315\/JFP-21-354_bb0020","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1111\/j.1750-3841.2010.01686.x","article-title":"Detection of E. coli O157:H7 from ground beef using Fourier transform infrared (FT-IR) spectroscopy and chemometrics","volume":"75","author":"Davis,","year":"2010","journal-title":"J. Food Sci"},{"key":"10.4315\/JFP-21-354_bb0025","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.bej.2009.07.013","article-title":"Application of flow cytometry to industrial microbial bioprocesses","volume":"48","author":"D\u00edaz,","year":"2009","journal-title":"Biochem. Eng. J"},{"key":"10.4315\/JFP-21-354_bb0030","doi-asserted-by":"crossref","first-page":"104992","DOI":"10.1016\/j.ultsonch.2020.104992","article-title":"Proteomic responses of spores of Bacillus subtilis to thermosonication involve large-scale alterations in metabolic pathways","volume":"64","author":"Fan,","year":"2020","journal-title":"Ultrason. Sonochem"},{"key":"10.4315\/JFP-21-354_bb0035","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1556\/AAlim.32.2003.4.5","article-title":"Effects of pasteurizing levels of high hydrostatic pressure on Bacillus subtilis luxAB spores","volume":"32","author":"Farkas,","year":"2003","journal-title":"Acta Aliment"},{"key":"10.4315\/JFP-21-354_bb0040","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.jddst.2017.12.009","article-title":"Effect of hydroxypropyl-\u03b2-cyclodextrin on lipid membrane fluidity, stability and freeze-drying of liposomes","volume":"44","author":"Gharib,","year":"2018","journal-title":"J. Drug. Deliv. Sci. Technol"},{"key":"10.4315\/JFP-21-354_bb0045","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.lwt.2018.12.005","article-title":"Changes process in the cellular structures and constituents of Lactobacillus bulgaricus sp1.1 during spray drying","volume":"102","author":"Gong,","year":"2018","journal-title":"LWT - Food Sci. Technol"},{"key":"10.4315\/JFP-21-354_bb0050","first-page":"69","article-title":"Classification and identification of bacteria by Fourier-transform infrared spectroscopy","volume":"137","author":"Helm,","year":"1991","journal-title":"J. Gen. Microbiol"},{"key":"10.4315\/JFP-21-354_bb0055","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/S0273-2300(03)00029-1","article-title":"Use of ADME studies to confirm the safety of \u03b5-polylysine as a preservative in food","volume":"37","author":"Hiraki,","year":"2003","journal-title":"Regul. Toxicol. Pharmacol"},{"key":"10.4315\/JFP-21-354_bb0060","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1002\/jsfa.9198","article-title":"An investigation of inactivation mechanisms of Bacillus amyloliquefaciens spores in non-thermal plasma of ambient air","volume":"99","author":"Huang,","year":"2019","journal-title":"J. Sci. Food Agric"},{"key":"10.4315\/JFP-21-354_bb0065","doi-asserted-by":"crossref","first-page":"7758","DOI":"10.1128\/AEM.02204-14","article-title":"The antimicrobial mechanism of action of epsilon-poly-l-lysine","volume":"80","author":"Hyldgaard,","year":"2014","journal-title":"Appl. Environ. Microbiol"},{"key":"10.4315\/JFP-21-354_bb0070","doi-asserted-by":"crossref","first-page":"28155","DOI":"10.1039\/C8CP04610G","article-title":"Relationship between water permeation and flip-flop motion in a bilayer membrane","volume":"20","author":"Inokuchi,","year":"2018","journal-title":"Phys. Chem. Chem. Phys"},{"key":"10.4315\/JFP-21-354_bb0075","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.mimet.2009.05.010","article-title":"Flow cytometric analysis of Salmonella enterica serotype Typhimurium inactivated with supercritical carbon dioxide","volume":"78","author":"Kim,","year":"2009","journal-title":"J. Microbiol. Methods"},{"key":"10.4315\/JFP-21-354_bb0080","doi-asserted-by":"crossref","first-page":"652","DOI":"10.3389\/fmicb.2015.00652","article-title":"High pressure thermal inactivation of Clostridium botulinum type E endospores\u2014kinetic modeling and mechanistic insights","volume":"6","author":"Lenz,","year":"2015","journal-title":"Front. Microbiol"},{"key":"10.4315\/JFP-21-354_bb0085","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.postharvbio.2018.08.009","article-title":"The modes of action of epsilon-polylysine (\u03b5-PL) against Botrytis cinerea in jujube fruit","volume":"147","author":"Li,","year":"2019","journal-title":"Postharvest Biol. Technol"},{"key":"10.4315\/JFP-21-354_bb0090","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1016\/j.bcmd.2005.06.009","article-title":"Molecular and chemical characterization of blood cells by infrared spectroscopy: a new optical tool in hematology","volume":"35","author":"Liu,","year":"2005","journal-title":"Blood Cells Mol. Dis"},{"key":"10.4315\/JFP-21-354_bb0095","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1111\/j.1365-2141.2006.06474.x","article-title":"Biomolecular characterisation of leucocytes by infrared spectroscopy","volume":"136","author":"Liu,","year":"2007","journal-title":"Br. J. Haematol"},{"key":"10.4315\/JFP-21-354_bb0100","doi-asserted-by":"crossref","first-page":"102523","DOI":"10.1016\/j.ifset.2020.102523","article-title":"Combined high pressure and heat treatment effectively disintegrates spore membranes and inactivates Alicyclobacillus acidoterrestris spores in acidic fruit juice beverage","volume":"66","author":"Luong,","year":"2020","journal-title":"Innov. Food Sci. Emerg. Technol"},{"key":"10.4315\/JFP-21-354_bb0105","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/j.ifset.2007.06.010","article-title":"Flow cytometric assessment of Bacillus spore response to high pressure and heat","volume":"8","author":"Mathys,","year":"2007","journal-title":"Innov. Food Sci. Emerg. Technol"},{"key":"10.4315\/JFP-21-354_bb0110","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.lwt.2015.12.010","article-title":"Combined effects of ultra-high hydrostatic pressure and mild heat on the inactivation of Bacillus subtilis","volume":"68","author":"Meng,","year":"2016","journal-title":"LWT - Food Sci. Technol"},{"key":"10.4315\/JFP-21-354_bb0115","doi-asserted-by":"crossref","first-page":"e0180680","DOI":"10.1371\/journal.pone.0180680","article-title":"FTIR spectroscopy of whole cells for the monitoring of yeast apoptosis mediated by p53 over-expression and its suppression by Nigella sativa extracts","volume":"12","author":"Mihoubi,","year":"2017","journal-title":"PLoS One"},{"key":"10.4315\/JFP-21-354_bb0120","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1002\/bit.22849","article-title":"Germination and inactivation of Bacillus subtilis spores induced by moderate hydrostatic pressure","volume":"107","author":"Minh,","year":"2010","journal-title":"Biotechnol. Bioeng"},{"key":"10.4315\/JFP-21-354_bb0125","doi-asserted-by":"crossref","first-page":"3122","DOI":"10.3389\/fmicb.2019.03122","article-title":"Understanding the effects of high pressure on bacterial spores using synchrotron infrared spectroscopy","volume":"10","author":"Modugno,","year":"2020","journal-title":"Front. Microbiol"},{"key":"10.4315\/JFP-21-354_bb0130","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/S0167-7012(99)00130-X","article-title":"Flow cytometric techniques to characterise physiological states of Acinetobacter calcoaceticus","volume":"40","author":"M\u00fcller,","year":"2000","journal-title":"J. Microbiol. Methods"},{"key":"10.4315\/JFP-21-354_bb0135","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1038\/351081a0","article-title":"Microbiological characterizations by FT-IR spectroscopy","volume":"351","author":"Naumann,","year":"1991","journal-title":"Nature"},{"key":"10.4315\/JFP-21-354_bb0140","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1146\/annurev-food-032519-051632","article-title":"Endospore inactivation by emerging technologies: a review of target structures and inactivation mechanisms","volume":"11","author":"Reineke,","year":"2020","journal-title":"Annu. Rev. Food Sci. Technol"},{"key":"10.4315\/JFP-21-354_bb0145","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1007\/s12013-013-9537-4","article-title":"Gabaergic pharmacological activity of propofol related compounds as possible enhancers of general anesthetics and interaction with membranes","volume":"67","author":"Reiner,","year":"2013","journal-title":"Cell Biochem. Biophys"},{"key":"10.4315\/JFP-21-354_bb0150","doi-asserted-by":"crossref","first-page":"108781","DOI":"10.1016\/j.lwt.2019.108781","article-title":"Effect of high pressure processing combined with temperature on the inactivation and germination of Alicyclobacillus acidoterrestris spores: influence of heat-shock on the counting of survivors","volume":"118","author":"Ribeiro,","year":"2020","journal-title":"LWT - Food Sci. Technol"},{"key":"10.4315\/JFP-21-354_bb0155","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.snb.2012.10.139","article-title":"Cell membrane morphology analysis using an infrared sensor system","volume":"179","author":"Sander,","year":"2013","journal-title":"Sens. Actuators B Chem"},{"key":"10.4315\/JFP-21-354_bb0160","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.3389\/fmicb.2015.01105","article-title":"Cell membrane fatty acid changes and desaturase expression of Saccharomyces bayanus exposed to high pressure homogenization in relation to the supplementation of exogenous unsaturated fatty acids","volume":"6","author":"Serrazanetti,","year":"2015","journal-title":"Front. Microbiol"},{"key":"10.4315\/JFP-21-354_bb0165","doi-asserted-by":"crossref","DOI":"10.1128\/microbiolspec.TBS-0003-2012","article-title":"Spore resistance properties","volume":"2","author":"Setlow,","year":"2014","journal-title":"Microbiol. Spectr."},{"key":"10.4315\/JFP-21-354_bb0170","doi-asserted-by":"crossref","first-page":"2597","DOI":"10.3389\/fmicb.2020.596092","article-title":"Bacterial spore mRNA\u2014What's up with that?","volume":"11","author":"Setlow,","year":"2020","journal-title":"Front. Microbiol"},{"key":"10.4315\/JFP-21-354_bb0175","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1111\/1541-4337.12348","article-title":"Continuous versus discontinuous ultra-high-pressure systems for food sterilization with focus on ultra-high-pressure homogenization and high-pressure thermal sterilization: a review","volume":"17","author":"Sevenich,","year":"2018","journal-title":"Compr. Rev. Food Sci. Food Saf"},{"key":"10.4315\/JFP-21-354_bb0180","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.ifset.2016.09.013","article-title":"A scientific and interdisciplinary approach for high pressure processing as a future toolbox for safe and high quality products: a review","volume":"38","author":"Sevenich,","year":"2016","journal-title":"Innov. Food Sci. Emerg. Technol"},{"key":"10.4315\/JFP-21-354_bb0185","doi-asserted-by":"crossref","first-page":"5427","DOI":"10.1007\/s00253-020-10575-2","article-title":"Transcriptional changes involved in inhibition of biofilm formation by \u03b5-polylysine in Salmonella Typhimurium","volume":"104","author":"Shen,","year":"2020","journal-title":"Appl. Microbiol. Biotechnol"},{"key":"10.4315\/JFP-21-354_bb0190","first-page":"1917","article-title":"Inactivation of bacteriophages by \u03b5-poly-l-lysine produced by Streptomyces","volume":"46","author":"Shima,","year":"1982","journal-title":"Agric. Biol. Chem"},{"key":"10.4315\/JFP-21-354_bb0195","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/0304-4157(78)90010-2","article-title":"Fluidity parameters of lipid regions determined by fluorescence polarization","volume":"515","author":"Shinitzky,","year":"1978","journal-title":"Biochim. Biophys. Acta"},{"key":"10.4315\/JFP-21-354_bb0200","doi-asserted-by":"crossref","first-page":"2922","DOI":"10.1002\/jsfa.9505","article-title":"The inhibition mechanism of \u03b5-polylysine against Bacillus cereus emerging in surimi gel during refrigerated storage","volume":"99","author":"Su,","year":"2019","journal-title":"J. Sci. Food Agric"},{"key":"10.4315\/JFP-21-354_bb0205","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/S0165-022X(03)00076-9","article-title":"Fluorescent probes for bacterial cytoplasmic membrane research","volume":"57","author":"Trevors,","year":"2003","journal-title":"J. Biochem. Biophys. Methods"},{"key":"10.4315\/JFP-21-354_bb0210","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.mimet.2013.11.009","article-title":"1,6-Diphenyl-1,3,5-hexatrine as a reporter of inner spore membrane fluidity in Bacillus subtilis and Alicyclobacillus acidoterrestris","volume":"96","author":"Voss,","year":"2014","journal-title":"J. Microbiol. Methods"},{"key":"10.4315\/JFP-21-354_bb0215","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1039\/C8EN00133B","article-title":"Iron oxide nanowire\u2013based filter for inactivation of airborne bacteria","volume":"5","author":"Wang,","year":"2018","journal-title":"Environ. Sci. Nano"},{"key":"10.4315\/JFP-21-354_bb0220","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1128\/AEM.66.1.257-261.2000","article-title":"Comparative study of pressure- and nutrient-induced germination of Bacillus subtilis spores","volume":"66","author":"Wuytack,","year":"2000","journal-title":"Appl. Environ. Microbiol"},{"key":"10.4315\/JFP-21-354_bb0225","doi-asserted-by":"crossref","first-page":"M144","DOI":"10.1111\/1750-3841.13169","article-title":"Effects of electrolyzed oxidizing water on inactivation of Bacillus subtilis and Bacillus cereus spores in suspension and on carriers","volume":"81","author":"Zhang,","year":"2016","journal-title":"J. Food Sci"},{"key":"10.4315\/JFP-21-354_bb0230","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.ijfoodmicro.2012.10.009","article-title":"Inactivation of Bacillus subtilis spores by combining high-pressure thermal sterilization and ethanol","volume":"160","author":"Zhang,","year":"2012","journal-title":"Int. J. Food Microbiol"}],"container-title":["Journal of Food Protection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0362028X22067850?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0362028X22067850?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/meridian.allenpress.com\/jfp\/article-pdf\/85\/3\/390\/3032178\/i1944-9097-85-3-390.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/meridian.allenpress.com\/jfp\/article-pdf\/85\/3\/390\/3032178\/i1944-9097-85-3-390.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T07:14:30Z","timestamp":1753859670000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0362028X22067850"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3]]},"references-count":45,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,3]]}},"alternative-id":["S0362028X22067850"],"URL":"https:\/\/doi.org\/10.4315\/jfp-21-354","relation":{},"ISSN":["0362-028X"],"issn-type":[{"value":"0362-028X","type":"print"}],"subject":[],"published":{"date-parts":[[2022,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"High Pressure Thermal Sterilization and \u03b5-Polylysine Synergistically Inactivate Bacillus subtilis Spores by Damaging the Inner Membrane","name":"articletitle","label":"Article Title"},{"value":"Journal of Food Protection","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.4315\/JFP-21-354","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2022 International Association for Food Protection. Published by Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}]}}