{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T17:01:48Z","timestamp":1783702908829,"version":"3.55.0"},"reference-count":0,"publisher":"SMW Supporting Association","issue":"3940","license":[{"start":{"date-parts":[[2010,9,27]],"date-time":"2010-09-27T00:00:00Z","timestamp":1285545600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Swiss Med Wkly"],"abstract":"<jats:p>BACKGROUND: PCR in the cerebrospinal fluid (CSF) has become  the sole method used for the diagnosis of herpes simplex encephalitis (HSE).  Nevertheless, PCR results may sometimes be false negative, and in this  situation other techniques may be useful.\n        METHODS: 3 patients hospitalised for meningoencephalitis  with fever showed a negative result for herpes simplex virus (HSV) PCR in their  CSF. We then performed a detection of intrathecal anti-HSV immunoglobulins  (IgGs) in the CSF and analysed their level in relation to those in the serum,  compared to albumin.\n        RESULTS: We confirmed that IgG synthesis was the direct  consequence of an immune system reaction in the 3 patients\u2019 CSF. These results  were consistent with clinical signs and neurodiagnostic procedures. They  prompted us to continue the treatment, which would have been stopped following  the negative PCR results. The clinical progression was favourable for all  patients.\n        CONCLUSIONS: PCR, which many physicians now consider the  gold standard for the detection of HSV, may sometimes yield false negative  results, i.e. when performed too early after the disease onset or when the  viral load is too low. The method described here, although positive a few days  after PCR, may prove helpful in the diagnosis of HSE for patients with negative  HSV PCR in the CSF.<\/jats:p>","DOI":"10.4414\/smw.2010.13107","type":"journal-article","created":{"date-parts":[[2011,10,27]],"date-time":"2011-10-27T03:48:08Z","timestamp":1319687288000},"page":"w13107","source":"Crossref","is-referenced-by-count":13,"title":["Intrathecal synthesis of specific antibodies as a marker of herpes simplex encephalitis in  patients with negative PCR"],"prefix":"10.57187","volume":"140","author":[{"given":"E","family":"Denes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Labach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Durox","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Adoukonou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Weinbreck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Magy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Ranger-Rogez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"36756","published-online":{"date-parts":[[2010,9,27]]},"container-title":["Swiss Medical Weekly"],"original-title":[],"link":[{"URL":"https:\/\/smw.ch\/index.php\/smw\/article\/download\/1198\/1280","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/smw.ch\/index.php\/smw\/article\/download\/1198\/1281","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/smw.ch\/index.php\/smw\/article\/download\/1198\/1280","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,15]],"date-time":"2025-08-15T02:28:23Z","timestamp":1755224903000},"score":1,"resource":{"primary":{"URL":"https:\/\/smw.ch\/index.php\/smw\/article\/view\/1198"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,9,27]]},"references-count":0,"journal-issue":{"issue":"3940","published-online":{"date-parts":[[2010,9,27]]}},"URL":"https:\/\/doi.org\/10.4414\/smw.2010.13107","relation":{},"ISSN":["1424-3997"],"issn-type":[{"value":"1424-3997","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,9,27]]}}}