{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T17:10:04Z","timestamp":1759684204562,"version":"build-2065373602"},"reference-count":0,"publisher":"Pakistan Medical Association","issue":"7 (July) (Supple-02)","license":[{"start":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T00:00:00Z","timestamp":1759536000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Pak Med Assoc"],"abstract":"<jats:p>Objective: To investigate the effect and possible mechanism of Huanghou Zhixie dropping pills in ulcerative colitistreatment.Method: The study was conducted at the Inner Mongolia Medical University, Hohhot, China, between March and November2022, and comprised specific pathogen-free Bagg albino (C57BL6) adult female mice. Ulcerative colitis was induced usingdextran sulfate sodium. The mice were divided randomly into control group, model group, low-dose Huanghou Zhixiedropping pills HZDP-L group, medium-dose Huanghou Zhixie dropping pills HZDP-M group, high-dose Huanghou Zhixiedropping pills HZDP-H group, and mesalazine group. The quality of the model was verified through Disease Activity Index,Colonic Mucosal Damage Index, and Histopathological) analysis. Enzyme-linked immunosorbent assay was used to measureinflammatory factors related to ulcerative colitis, including Interleukin-1 beta (IL-1beta), Transforming Growth Factor-beta (TGF-beta), Interleukin-4 (IL-4), Tumour Necrosis Factor-alpha (TNF-alpha), Interleukin-6 (IL-6) and Interleukin-10 (IL-10). Western blotanalysis was conducted to examine the relevant factors, such as cyclooxygenase-2, inducible nitric oxide synthase, Protein65 (P65), Mitogen-Activated Protein Kinase (MAPK), Phosphorylated Mitogen-Activated Protein Kinase (p-MAPK) andPhosphorylated Protein 65 (p-P65). Data was analysed using SPSS 20.Results: Of the 36 female mice aged 8 weeks and having mean weight 18\u00b12g, 6(16.66%) were in each of the 6 groups.Huanghou Zhixie dropping pills significantly reduced dextran sulfate sodium-induced ulcerative colitis symptoms, weightlossand colon damage (p&lt;0.05). Huanghou Zhixie dropping pills decreased the expression of pro-inflammatory TNF-alpha,IL-1beta, TGF-beta, and IL-6 factors, and increased the expression of anti-inflammatory IL-4 and IL-10 factors in the colon tissue ofthe mice (p&lt;0.05). Additionally, Huanghou Zhixie dropping pills reduced the expression of cyclooxygenase-2, induciblenitric oxide synthase, P-MAPK and P-P65 in the colon tissue (p&lt;0.05).Conclusion: Huanghou Zhixie dropping pills alleviated colitis by modulating inflammatory factors through the Mitogen-Activated Protein Kinase\/Nuclear Factor kappa-light-chain-enhancer of activated B cells (MAPK\/NF-kB) signalling pathway.The Huanghou Zhixie dropping pills could be a potential therapeutic option for ulcerative colitis treatment, offering a novelapproach to managing the disease by targeting specific inflammatory pathways.Keywords: HZDP, Ulcerative colitis, MAPK\/NF-kB signaling pathway, Inflammatory factors.<\/jats:p>","DOI":"10.47391\/jpma.srph-04","type":"journal-article","created":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T16:49:27Z","timestamp":1759682967000},"source":"Crossref","is-referenced-by-count":0,"title":["Huanghou Zhixie dropping pills alleviate ulcerative colitis via MAPK\/ NF-kB signalling pathway"],"prefix":"10.47391","volume":"75","author":[{"family":"Zhen Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Yan Mei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Guosheng Xing","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Mingxing Hou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"26547","published-online":{"date-parts":[[2025,10,4]]},"container-title":["Journal of the Pakistan Medical Association"],"original-title":[],"link":[{"URL":"https:\/\/www.jpma.org.pk\/index.php\/public_html\/article\/download\/33188\/4425","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.jpma.org.pk\/index.php\/public_html\/article\/download\/33188\/4425","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T16:49:27Z","timestamp":1759682967000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jpma.org.pk\/index.php\/public_html\/article\/view\/33188"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,4]]},"references-count":0,"journal-issue":{"issue":"7 (July) (Supple-02)","published-online":{"date-parts":[[2025,10,5]]}},"URL":"https:\/\/doi.org\/10.47391\/jpma.srph-04","relation":{},"ISSN":["0030-9982","0030-9982"],"issn-type":[{"value":"0030-9982","type":"electronic"},{"value":"0030-9982","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10,4]]}}}