{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:38:01Z","timestamp":1787031481992,"version":"build-2736575974"},"reference-count":0,"publisher":"Care Comm","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ceska Gynekol"],"published-print":{"date-parts":[[2024,12,31]]},"abstract":"<jats:p>Aim: Within the in vitro fertilization (IVF) process, to evaluate the possibility of using the state of the meiotic spindle of oocytes as an indicator of maturity in order to optimize the timing of vitrification. Patients and methods: In the presented report, the cause of couple infertility was a combination of a 38-year-old female and 43-year-old male with azoospermia, which was an indication for oocyte vitrification. Oocyte polar bodies and optically birefringent meiotic spindles were visualized by polarized light microscopy and their states and relative positions were used as indicators of oocyte maturation, i.e. readiness for vitrification. Oocytes which had an angle between the polar body and meiotic spindle \u03b1 \u2264 30\u00b0 or had a poorly visible meiotic spindle were vitrified 4 hours post-meiotic spindle evaluation (8 hours after ovum pick-up). Results: After thawing, oocytes were fully maturated and prepared for intracytoplasmic sperm injection (ICSI). Fol lowing treatment with tamoxifen, vital sperm were retrieved from the patient\u2019s partner, the oocytes were thawed and fertilized with the obtained sperm, with subsequent embryo transfer and delivery of a healthy baby at term. Conclusion: The meiotic spindle can be used as an oocyte maturation pointer in older women. After thawing, the oocytes were fully matured and ready for fertilization by ICSI. \n\nKey words: oocyte \u2013 meiotic spindle \u2013 polar body \u2013 IVF \u2013 pregnancy<\/jats:p>","DOI":"10.48095\/cccg2024475","type":"journal-article","created":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T09:02:29Z","timestamp":1737018149000},"page":"475-478","source":"Crossref","is-referenced-by-count":1,"title":["Meiotic spindle as a marker of optimal time for oocyte vitrification \u2013 presentation of successful infertility treatment and literature review"],"prefix":"10.48095","volume":"89","author":[{"name":"Center for Assisted Reproduction, Department of Gynecology, Obstetrics and Neonatology, 1st Faculty of Medicine, Charles University and General Hospital in Prague, Czech Republic","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jarom\u00edr","family":"Ma\u0161ata","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Tepl\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Jirsov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"P\u0161eni\u010dkov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Moosov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Fajmonov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irena","family":"Kratochvilov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"28151","published-online":{"date-parts":[[2024,12,31]]},"container-title":["\u010cesk\u00e1 gynekologie"],"original-title":["Meiotick\u00e9 v\u0159et\u00e9nko jako marker optim\u00e1ln\u00edho \u010dasu pro vitrifikaci oocyt\u016f \u2013 p\u0159\u00edpad \u00fasp\u011b\u0161n\u00e9 l\u00e9\u010dby neplodnosti a p\u0159ehled literatury"],"deposited":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T09:02:35Z","timestamp":1737018155000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cs-gynekologie.cz\/en\/journals\/czech-gynaecology\/2024-6-8\/meiotic-spindle-as-a-marker-of-optimal-time-for-oocyte-vitrification-presentation-of-successful-infertility-treatment-and-literature-review-139549"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,31]]},"references-count":0,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2024,12,31]]},"published-print":{"date-parts":[[2024,12,31]]}},"URL":"https:\/\/doi.org\/10.48095\/cccg2024475","relation":{},"ISSN":["1210-7832","1805-4455"],"issn-type":[{"value":"1210-7832","type":"print"},{"value":"1805-4455","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,31]]}}}