{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T12:30:54Z","timestamp":1761913854949,"version":"build-2065373602"},"reference-count":0,"publisher":"Care Comm","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ceska Gynekol"],"published-print":{"date-parts":[[2025,10,30]]},"abstract":"<jats:p>Objective: The aim of this study was to validate the methodology of ovarian tissue cryopreservation using the slow freezing technique as a fertility preservation approach and to assess its potential implementation in clinical practice at the Assisted Reproduction Centre of the University Hospital Olomouc. In parallel, the technical procedure of cryopreservation was optimized and standardized. Materials and methods: The study was conducted between April 2022 and December 2024 at the Department of Obstetrics and Gynecology, University Hospital Olomouc, and included six transgender patients aged 19\u201325 years who underwent laparoscopic hysterectomy with bilateral adnexectomy as part of gender-affirming surgery. Ovarian tissue obtained during the procedure was immediately processed and cryopreserved using the slow freezing method at the Assisted Reproduction Centre of the University Hospital Olomouc. Histopathological evaluation of the ovarian tissue was performed both prior to cryopreservation and after thawing at the Department of Clinical and Molecular Pathology. Results: Morphological assessment of the ovarian tissue after cryopreservation confirmed preservation of structural characteristics of follicles and stromal components, without signs of significant degeneration. Conclusion: The results confirm that the applied slow freezing protocol for ovarian tissue cryopreservation is appropriate and sufficiently gentle for clinical use. This method represents a reliable option for fertility preservation in patients undergoing gonadectomy, with potential applications in subsequent autologous transplantation or in\u2005vitro follicle culture. \n\nKey words: fertility preservation \u2013 ovarian tissue cryopreservation \u2013 slow-freezing \u2013 oncofertility \u2013 assisted reproduction \u2013 in\u2005vitro fertilization \u2013 transgender<\/jats:p>","DOI":"10.48095\/cccg2025349","type":"journal-article","created":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T12:26:53Z","timestamp":1761913613000},"page":"349-359","source":"Crossref","is-referenced-by-count":0,"title":["Comparative analysis of the histological architecture of ovarian tissue following slow-freezing cryopreservation"],"prefix":"10.48095","volume":"90","author":[{"name":"Department of Obstetrics and Gynecology, Faculty of Medicine, Palacky University and University Hospital Olomouc, Czech Republic","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veronika","family":"Hon\u010dov\u00e1","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Vodi\u010dka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zuzana","family":"Slobodov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Radovan","family":"Pilka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"28151","published-online":{"date-parts":[[2025,10,31]]},"container-title":["\u010cesk\u00e1 gynekologie"],"original-title":["Srovn\u00e1n\u00ed histologick\u00e9 struktury kryokonzervovan\u00e9 ovari\u00e1ln\u00ed tk\u00e1n\u011b metodou pomal\u00e9ho zamra\u017een\u00ed"],"deposited":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T12:26:54Z","timestamp":1761913614000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cs-gynekologie.cz\/en\/journals\/czech-gynaecology\/2025-5-5\/comparative-analysis-of-the-histological-architecture-of-ovarian-tissue-following-slow-freezing-cryopreservation-141986"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,30]]},"references-count":0,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2025,10,31]]},"published-print":{"date-parts":[[2025,10,30]]}},"URL":"https:\/\/doi.org\/10.48095\/cccg2025349","relation":{},"ISSN":["1210-7832","1805-4455"],"issn-type":[{"value":"1210-7832","type":"print"},{"value":"1805-4455","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,30]]}}}