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Wang Z, Chen Z, Jiang Z, Luo P, Liu L, et al. 2019. Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents. Nature Communications<\/ext-link><\/i> 10:2538<\/p>","DOI":"10.1038\/s41467-019-10386-8"},{"key":"key-10.48130\/els-0026-0009-2","doi-asserted-by":"publisher","unstructured":"

Wang L, Tian S, Ruan S, Wei J, Wei S, et al. 2024. Neuroprotective effects of cordycepin on MPTP-induced Parkinson's disease mice via suppressing PI3K\/AKT\/mTOR and MAPK-mediated neuroinflammation. Free Radical Biology and Medicine<\/ext-link><\/i> 216:60\u221277<\/p>","DOI":"10.1016\/j.freeradbiomed.2024.02.023"},{"key":"key-10.48130\/els-0026-0009-3","doi-asserted-by":"publisher","unstructured":"

Gao Y, Chen DL, Zhou M, Zheng ZS, He MF, et al. 2020. Cordycepin enhances the chemosensitivity of esophageal cancer cells to cisplatin by inducing the activation of AMPK and suppressing the AKT signaling pathway. Cell Death & Disease<\/ext-link><\/i> 11:866<\/p>","DOI":"10.1038\/s41419-020-03079-4"},{"key":"key-10.48130\/els-0026-0009-4","doi-asserted-by":"publisher","unstructured":"

Cunningham KG, Manson W, Spring FS, Hutchinson SA. 1950. Cordycepin, a metabolic product isolated from cultures of Cordyceps militaris<\/i> (Linn.) link. Nature<\/ext-link><\/i> 166:949<\/p>","DOI":"10.1038\/166949a0"},{"key":"key-10.48130\/els-0026-0009-5","doi-asserted-by":"publisher","unstructured":"

Xia Y, Luo F, Shang Y, Chen P, Lu Y, et al. 2017. Fungal cordycepin biosynthesis is coupled with the production of the safeguard molecule pentostatin. Cell Chemical Biology<\/ext-link><\/i> 24:1479\u22121489.e4<\/p>","DOI":"10.1016\/j.chembiol.2017.09.001"},{"key":"key-10.48130\/els-0026-0009-6","doi-asserted-by":"publisher","unstructured":"

Duan XY, Tian Y, Song ZQ, Song LP, Lin WB, et al. 2022. High-level de novo biosynthesis of cordycepin by systems metabolic engineering in Yarrowia lipolytica. Bioresource Technology<\/ext-link><\/i> 363:127862<\/p>","DOI":"10.1016\/j.biortech.2022.127862"},{"key":"key-10.48130\/els-0026-0009-7","doi-asserted-by":"publisher","unstructured":"

Song Z, Lin W, Duan X, Song L, Wang C, et al. 2023. Increased cordycepin production in Yarrowia lipolytica<\/i> using combinatorial metabolic engineering strategies. ACS Synthetic Biology<\/ext-link><\/i> 12:780\u2212787<\/p>","DOI":"10.1021\/acssynbio.2c00570"},{"key":"key-10.48130\/els-0026-0009-8","doi-asserted-by":"publisher","unstructured":"

Zhang H, Chen P, Peng D, Zou F, Niu H, et al. 2026. Efficient production of cordycepin in engineered Saccharomyces cerevisiae<\/i>. Bioresource Technology<\/ext-link><\/i> 452:134565<\/p>","DOI":"10.1016\/j.biortech.2026.134565"},{"key":"key-10.48130\/els-0026-0009-9","doi-asserted-by":"publisher","unstructured":"

Tan H, Wang L, Wang H, Cheng Y, Li X, et al. 2023. Engineering Komagataella phaffii<\/i> to biosynthesize cordycepin from methanol which drives global metabolic alterations at the transcription level. Synthetic and Systems Biotechnology<\/ext-link><\/i> 8:242\u2212252<\/p>","DOI":"10.1016\/j.synbio.2023.03.003"},{"key":"key-10.48130\/els-0026-0009-10","doi-asserted-by":"publisher","unstructured":"

Wang J, Jiang W, Liang C, Zhu L, Li Y, et al. 2021. Overproduction of \u03b1-Farnesene in Saccharomyces cerevisiae by Farnesene Synthase Screening and Metabolic Engineering. Journal of Agricultural and Food Chemistry<\/ext-link><\/i> 69:3103\u22123113<\/p>","DOI":"10.1021\/acs.jafc.1c00008"},{"key":"key-10.48130\/els-0026-0009-11","doi-asserted-by":"publisher","unstructured":"

Yuan J, Ching CB. 2015. Combinatorial assembly of large biochemical pathways into yeast chromosomes for improved production of value-added compounds. ACS Synthetic Biology<\/ext-link><\/i> 4:23\u221231<\/p>","DOI":"10.1021\/sb500079f"},{"key":"key-10.48130\/els-0026-0009-12","doi-asserted-by":"publisher","unstructured":"

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Chen X, Li C, Qiu X, Chen M, Xu Y, et al. 2025. CRISPR\/Cas9-based iterative multi-copy integration for improved metabolite yields in Saccharomyces cerevisiae<\/i>. Synthetic and Systems Biotechnology<\/ext-link><\/i> 10:629\u2212637<\/p>","DOI":"10.1016\/j.synbio.2025.02.016"},{"key":"key-10.48130\/els-0026-0009-17","doi-asserted-by":"publisher","unstructured":"

Ling R, Hou S, Mao X, Secundo F, Zhou W, et al. 2026. rDNA-mediated multicopy integration and gene dosage quantification system for microbial zeaxanthin biosynthesis. World Journal of Microbiology and Biotechnology<\/ext-link><\/i> 42:265<\/p>","DOI":"10.1007\/s11274-026-04993-z"},{"key":"key-10.48130\/els-0026-0009-18","doi-asserted-by":"publisher","unstructured":"

Thornton K, Kwan EX, Bubb KL, Paleologu L, Raghuraman MK, et al. 2025. rDNA copy number variation affects yeast fitness in response to different environments. Genetics<\/ext-link><\/i> 230:iyaf075<\/p>","DOI":"10.1093\/genetics\/iyaf075"},{"key":"key-10.48130\/els-0026-0009-19","doi-asserted-by":"publisher","unstructured":"

Yu D, Chen H, Zhang Y, Chen Y, Chen H, et al. 2026. Biosensor-guided indexing of genome evolution for boosting microbial chemical synthesis. Trends in Biotechnology<\/ext-link><\/i> 44:1641\u22121659<\/p>","DOI":"10.1016\/j.tibtech.2025.12.019"},{"key":"key-10.48130\/els-0026-0009-20","doi-asserted-by":"publisher","unstructured":"

Tang C, Zhang L, Wang J, Zou C, Zhang Y, et al. 2024. Engineering Saccharomyces boulardii<\/i> for probiotic supplementation of l-ergothioneine. Biotechnology Journal<\/ext-link><\/i> 19:e202400527<\/p>","DOI":"10.1002\/biot.202400527"},{"key":"key-10.48130\/els-0026-0009-21","doi-asserted-by":"publisher","unstructured":"

Chen Q, Luo W, Veach RA, Hickman AB, Wilson MH, et al. 2020. Structural basis of seamless excision and specific targeting by piggyBac transposase. Nature Communications<\/ext-link><\/i> 11:3446<\/p>","DOI":"10.1038\/s41467-020-17128-1"},{"key":"key-10.48130\/els-0026-0009-22","doi-asserted-by":"publisher","unstructured":"

Wang J, Ma J, Luo X, Wang S, Cai X, et al. 2025. Design and characterization of allantoin-inducible expression systems in budding yeast. Biotechnology for Biofuels and Bioproducts<\/ext-link><\/i> 18:26<\/p>","DOI":"10.1186\/s13068-025-02630-9"},{"key":"key-10.48130\/els-0026-0009-23","doi-asserted-by":"publisher","unstructured":"

Johnston M. 1987. A model fungal gene regulatory mechanism: the GAL genes of Saccharomyces cerevisiae. Microbiological Reviews<\/ext-link><\/i> 51:458\u2212476<\/p>","DOI":"10.1128\/mr.51.4.458-476.1987"},{"key":"key-10.48130\/els-0026-0009-24","doi-asserted-by":"publisher","unstructured":"

Gancedo JM. 1998. Yeast carbon catabolite repression. Microbiology and Molecular Biology Reviews<\/ext-link><\/i> 62:334\u2212361<\/p>","DOI":"10.1128\/mmbr.62.2.334-361.1998"},{"key":"key-10.48130\/els-0026-0009-25","doi-asserted-by":"publisher","unstructured":"

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Fan C, He N, Yuan J. 2024. Cascaded amplifying circuit enables sensitive detection of fungal pathogens. Biosensors and Bioelectronics<\/ext-link><\/i> 250:116058<\/p>","DOI":"10.1016\/j.bios.2024.116058"},{"key":"key-10.48130\/els-0026-0009-27","doi-asserted-by":"publisher","unstructured":"

Song J, Fan J, Fan C, He N, Ye X, et al. 2023. A layered genetic design enables the yeast galactose regulon to respond to cyanamide. ACS Synthetic Biology<\/ext-link><\/i> 12:2783\u22122788<\/p>","DOI":"10.1021\/acssynbio.3c00241"},{"key":"key-10.48130\/els-0026-0009-28","doi-asserted-by":"publisher","unstructured":"

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Yusa K, Zhou L, Li MA, Bradley A, Craig NL. 2011. A hyperactive piggyBac transposase for mammalian applications. Proceedings of the National Academy of Sciences of the United States of America<\/ext-link><\/i> 108:1531\u22121536<\/p>","DOI":"10.1073\/pnas.1008322108"},{"key":"key-10.48130\/els-0026-0009-30","doi-asserted-by":"publisher","unstructured":"

Mandell DJ, Lajoie MJ, Mee MT, Takeuchi R, Kuznetsov G, et al. 2015. Biocontainment of genetically modified organisms by synthetic protein design. Nature<\/ext-link><\/i> 518:55\u221260<\/p>","DOI":"10.1038\/nature14121"},{"key":"key-10.48130\/els-0026-0009-31","doi-asserted-by":"publisher","unstructured":"

Rovner AJ, Haimovich AD, Katz SR, Li Z, Grome MW, et al. 2015. Recoded organisms engineered to depend on synthetic amino acids. Nature<\/ext-link><\/i> 518:89\u221293<\/p>","DOI":"10.1038\/nature14095"},{"key":"key-10.48130\/els-0026-0009-32","doi-asserted-by":"publisher","unstructured":"

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