{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T11:10:09Z","timestamp":1745579409474,"version":"3.40.4"},"reference-count":0,"publisher":"Jaypee Brothers Medical Publishing","issue":"06","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JAPI"],"published-print":{"date-parts":[[2023,6,7]]},"abstract":"<jats:p>Background: Tuberculosis (TB) is a highly infectious disease causing millions of cases worldwide.  Though pulmonary TB is the most common form of infection, extrapulmonary cases are also very  rampant and are responsible for a large number of cases. But the diagnosis of extrapulmonary  cases is quite difficult because of varied manifestations and the paucibacillary nature of the  infection. Cartridge-based nucleic acid amplification test (CBNAAT) is a simple, rapid test that is  very efficient in the early diagnosis of these extrapulmonary cases [extrapulmonary TB (EPTB)]. Aim: A study was done to establish the usefulness of CBNAAT in the early diagnosis of EPTB cases. Materials and methods: A comparative study was conducted in a rural tertiary care hospital in West  Bengal, India, for 8 months (July 2021\u2013February 2022). Samples were collected from different sites  like pleural fluid, lymph nodes, cerebrospinal fluid (CSF), pus, ascitic fluid, and tissue aspirate and  subjected to both CBNAAT and smear staining and examination under a fluorescent microscope.  Positive samples were cultured, examined, and compared. Result: From 593 samples collected from different sites in suspected cases of EPTB\u201452 samples  were positive by CBNAAT, and six cases showed rifampicin resistance (RIF resistant). Smear staining  of the samples by auramine-rhodamine stains and examined under the fluorescent microscope  for acid-fast bacilli identifying 33 samples; the rest were negative. Slides showing acid-fast bacilli  were cultured on Lowenstein\u2013Jensen media. Conclusion: Cartridge-based nucleic acid amplification test (CBNAAT) is a very useful assay for  the early diagnosis of extrapulmonary cases as it can accurately identify false negative samples  by smear microscopy<\/jats:p>","DOI":"10.5005\/japi-11001-0260","type":"journal-article","created":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T09:25:34Z","timestamp":1693301134000},"page":"34-37","source":"Crossref","is-referenced-by-count":1,"title":["Diagnostic Approach to Extrapulmonary Tuberculosis by  Cartridge-based Nucleic Acid Amplification Test"],"prefix":"10.59556","volume":"71","author":[{"given":"Saswati","family":"Chattopadhyay","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanusri","family":"Biswas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abhra","family":"Banerjee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nabamita","family":"Chaudhury","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raston","family":"Mondal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arghya","family":"Nath","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2914","published-online":{"date-parts":[[2023,6,1]]},"container-title":["Journal of the Association of Physicians of India"],"original-title":[],"deposited":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T10:56:40Z","timestamp":1745578600000},"score":1,"resource":{"primary":{"URL":"https:\/\/japi.org\/article\/files\/JAPI0260_p34-37_compressed.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,1]]},"references-count":0,"journal-issue":{"issue":"06","published-online":{"date-parts":[[2023,6,1]]},"published-print":{"date-parts":[[2023,6,7]]}},"URL":"https:\/\/doi.org\/10.5005\/japi-11001-0260","relation":{},"ISSN":["0004-5772"],"issn-type":[{"type":"print","value":"0004-5772"}],"subject":[],"published":{"date-parts":[[2023,6,1]]}}}