{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T07:08:48Z","timestamp":1768720128026,"version":"3.49.0"},"reference-count":46,"publisher":"Jaypee Brothers Medical Publishing","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n<jats:sec>\n<jats:title>Aim<\/jats:title>\n<jats:p>Bacteria levels of necrotic teeth are greatly reduced after endodontic treatment procedures but the presence of persisting microorganisms leads to continuous efforts to develop materials with antimicrobial properties. The purpose of the study was to determine the antimicrobial activity of polyethylenimine (PEI) against common bacteria and yeasts, regarding planktonic cells and biofilm, and to clarify its antimicrobial mechanism of action through flow cytometry.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Materials and methods<\/jats:title>\n<jats:p>The antibiofilm and antimicrobial effect of PEI was determined against <jats:italic>Enterococcus faecalis, Staphylococcus aureus, Escherichia coli<\/jats:italic> and <jats:italic>Candida albicans<\/jats:italic> strains using reference protocols. The effect of PEI was evaluated regarding adhesion, biofilm formation and biofilm disaggregation. In order to understand PEI cellular effects flow cytometric analysis was performed with different fluorescent markers.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>It was verified that minimal inhibitory concentrations (MIC) values and minimal lethal concentrations (MLC) obtained for PEI were similar and ranged between 50 and 400 mg\/l, proving the microbicidal and fungicidal activity of this compound. Antibiofilm activity was also proved for all the microorganisms. Severe lesion of the membrane and cell depolarization was demonstrated.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion<\/jats:title>\n<jats:p>Polyethylenimine showed antimicrobial and antibiofilm activity against microorganisms often associated with apical periodontitis.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Clinical significance<\/jats:title>\n<jats:p>Theoretically, prolonging the antibacterial effects of materials used in endodontics may be interesting to help prevent reinfection and possibly to affect residual bacteria that survived the treatment procedures.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>How to cite this article<\/jats:title>\n<jats:p>Barros J, Dias A, Rodrigues MA, Pina-Vaz C, Lopes MA, Pina-Vaz I. Antibiofilm and Antimicrobial Activity of Polyethylenimine: An Interesting Compound for Endodontic Treatment. J Contemp Dent Pract 2015;16(6):427-432.<\/jats:p>\n<\/jats:sec>","DOI":"10.5005\/jp-journals-10024-1701","type":"journal-article","created":{"date-parts":[[2015,10,8]],"date-time":"2015-10-08T06:15:13Z","timestamp":1444284913000},"page":"427-432","source":"Crossref","is-referenced-by-count":20,"title":["Antibiofilm and Antimicrobial Activity of Polyethylenimine: An Interesting Compound for Endodontic Treatment"],"prefix":"10.5005","volume":"16","author":[{"given":"Irene","family":"Pina-Vaz","sequence":"first","affiliation":[]},{"given":"Joana","family":"Barros","sequence":"additional","affiliation":[]},{"given":"Ana","family":"Dias","sequence":"additional","affiliation":[]},{"given":"Miguel A","family":"Rodrigues","sequence":"additional","affiliation":[]},{"given":"Cid\u00e1lia","family":"Pina-Vaz","sequence":"additional","affiliation":[]},{"given":"Maria A","family":"Lopes","sequence":"additional","affiliation":[]}],"member":"2914","published-online":{"date-parts":[[2015]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/j.joen.2010.11.003","volume":"37:","author":"R\u00f4\u00e7as IN, Siqueira JF Jr.","year":"2011","journal-title":"J Endod"},{"key":"ref=2","doi-asserted-by":"crossref","unstructured":"R\u00f4\u00e7as IN, Siqueira JF Jr. In vivo antimicrobial effects of endodontic treatment procedures as assessed by molecular microbiologic techniques. 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Oral Microbiol Immunol 2008;23:384-390.","DOI":"10.1111\/j.1399-302X.2008.00440.x"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.1016\/j.joen.2009.08.003","volume":"35:","author":"Malkhassian G, Manzur AJ, Legner M, Fillery ED, Manek S, Basrani BR, Friedman S.","year":"2009","journal-title":"J Endod"},{"key":"ref=8","doi-asserted-by":"crossref","unstructured":"Malkhassian G, Manzur AJ, Legner M, Fillery ED, Manek S, Basrani BR, Friedman S. Antibacterial efficacy of MTAD final rinse and two percent chlorhexidine gel medication in teeth with apical periodontitis: a randomized double-blinded clinical trial. J Endod 2009;35:1483-1490.","DOI":"10.1016\/j.joen.2009.08.003"},{"key":"ref9","first-page":"93","volume":"32:","author":"Stuart CH, Schwartz SA, Beeson TJ, Owatz CB.","year":"2006","journal-title":"J Endod"},{"key":"ref=10","doi-asserted-by":"crossref","unstructured":"Stuart CH, Schwartz SA, Beeson TJ, Owatz CB. Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J Endod 2006;32:93-98.","DOI":"10.1016\/j.joen.2005.10.049"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1016\/j.joen.2010.04.007","volume":"36:","author":"Ricucci D, Siqueira JF Jr.","year":"2010","journal-title":"J Endod"},{"key":"ref=12","doi-asserted-by":"crossref","unstructured":"Ricucci D, Siqueira JF Jr. Biofilms and apical periodontitis: study of prevalence and association with clinical and histopathologic findings. J Endod 2010;36:1277-1288.","DOI":"10.1016\/j.joen.2010.04.007"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1111\/j.1601-1546.2004.00112.x","volume":"9:","author":"Svens\u00e4ter GBG.","year":"2004","journal-title":"Endodontic Topics"},{"key":"ref=14","doi-asserted-by":"crossref","unstructured":"Svens\u00e4ter GBG. Biofilms in endodontic infections. Endodontic Topics 2004;9:27-36.","DOI":"10.1111\/j.1601-1546.2004.00112.x"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1111\/j.1365-2591.2004.00891.x","volume":"38:","author":"Hems RS, Gulabivala K, Ng YL, Ready D, Spratt DA.","year":"2005","journal-title":"Int Endod J"},{"key":"ref=16","doi-asserted-by":"crossref","unstructured":"Hems RS, Gulabivala K, Ng YL, Ready D, Spratt DA. An in vitro evaluation of the ability of ozone to kill a strain of Enterococcus faecalis. Int Endod J 2005;38:22-29.","DOI":"10.1111\/j.1365-2591.2004.00891.x"},{"key":"ref17","author":"Martins MR, Carvalho MF, Vaz IP, Capelas JA, Martins MA, Gutknecht N.","year":"2012:","journal-title":"Lasers Med Sci"},{"key":"ref=18","doi-asserted-by":"crossref","unstructured":"Martins MR, Carvalho MF, Vaz IP, Capelas JA, Martins MA, Gutknecht N. Efficacy of Er, Cr: YSGG laser with endodontical radial firing tips on the outcome of endodontic treatment: blind randomized controlled clinical trial with 6-month evaluation. Lasers Med Sci 2012: doi:10.1007\/s10103-012-1172-1176.","DOI":"10.1007\/s10103-012-1172-6"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1016\/j.joen.2011.03.014","volume":"37:","author":"Rios A, He J, Glickman GN, Spears R, Schneiderman ED, Honeyman AL.","year":"2011","journal-title":"J Endod"},{"key":"ref=20","doi-asserted-by":"crossref","unstructured":"Rios A, He J, Glickman GN, Spears R, Schneiderman ED, Honeyman AL. Evaluation of photodynamic therapy using a light-emitting diode lamp against Enterococcus faecalis in extracted human teeth. J Endod 2011;37:856-859.","DOI":"10.1016\/j.joen.2011.03.014"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"3193","DOI":"10.1099\/00221287-143-10-3193","volume":"143:","author":"Helander IM, Alakomi HL, Latva-Kala K, Koski P.","year":"1997","journal-title":"Microbiology"},{"key":"ref=22","doi-asserted-by":"crossref","unstructured":"Helander IM, Alakomi HL, Latva-Kala K, Koski P. Polyethyleneimine is an effective permeabilizer of gram-negative bacteria. Microbiology 1997;143:3193-3199.","DOI":"10.1099\/00221287-143-10-3193"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.jconrel.2011.09.059","volume":"157:","author":"Weber ND, Merkel OM, Kissel T, Munoz-Fernandez MA.","year":"2012","journal-title":"J Control Release"},{"key":"ref=24","doi-asserted-by":"crossref","unstructured":"Weber ND, Merkel OM, Kissel T, Munoz-Fernandez MA. PEGylated poly(ethylene imine) copolymer-delivered siRNA inhibits HIV replication in vitro. J Control Release 2012;157:55-63.","DOI":"10.1016\/j.jconrel.2011.09.059"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.jconrel.2008.05.016","volume":"130:","author":"Breunig M, Hozsa C, Lungwitz U, Watanabe K, Umeda I, Kato H, Goepferich A.","year":"2008","journal-title":"J Control Release"},{"key":"ref=26","doi-asserted-by":"crossref","unstructured":"Breunig M, Hozsa C, Lungwitz U, Watanabe K, Umeda I, Kato H, Goepferich A. Mechanistic investigation of poly(ethylene imine)-based siRNA delivery: disulfide bonds boost intracellular release of the cargo. J Control Release 2008;130:57-63.","DOI":"10.1016\/j.jconrel.2008.05.016"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.jcis.2011.12.049","volume":"370:","author":"Bellettini IC, Nandi LG, Eising R, Domingos JB, Machado VG, Minatti E.","year":"2012","journal-title":"J Colloid Interface Sci"},{"key":"ref=28","doi-asserted-by":"crossref","unstructured":"Bellettini IC, Nandi LG, Eising R, Domingos JB, Machado VG, Minatti E. Properties of aqueous solutions of hydrophobically modified polyethylene imines in the absence and presence of sodium dodecylsulfate. J Colloid Interface Sci 2012;370:94-101.","DOI":"10.1016\/j.jcis.2011.12.049"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.mimet.2005.03.014","volume":"63:","author":"Honraet K, Goetghebeur E, Nelis HJ.","year":"2005","journal-title":"J Microbiol Methods"},{"key":"ref=30","doi-asserted-by":"crossref","unstructured":"Honraet K, Goetghebeur E, Nelis HJ. Comparison of three assays for the quantification of Candida biomass in suspension and CDC reactor grown biofilms. J Microbiol Methods 2005;63:287-289.","DOI":"10.1016\/j.mimet.2005.03.014"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1007\/s11046-010-9316-3","volume":"170:","author":"De Prijck K, De Smet N, Coenye T, Schacht E, Nelis HJ.","year":"2010","journal-title":"Mycopathologia"},{"key":"ref=32","doi-asserted-by":"crossref","unstructured":"De Prijck K, De Smet N, Coenye T, Schacht E, Nelis HJ. Prevention of Candida albicans biofilm formation by covalently bound dimethylaminoethylmethacrylate and polyethylenimine. Mycopathologia 2010;170:213-221.","DOI":"10.1007\/s11046-010-9316-3"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1093\/jac\/dki264","volume":"56:","author":"Pina-vaz C, Rodrigues AG, Costa-de-Oliveira S, Ricardo E, Mardh PA.","year":"2005","journal-title":"J Antimicrob Chemother"},{"key":"ref=34","doi-asserted-by":"crossref","unstructured":"Pina-vaz C, Rodrigues AG, Costa-de-Oliveira S, Ricardo E, Mardh PA. Potent synergic effect between ibuprofen and azoles on Candida resulting from blockade of efflux pumps as determined by FUN-1 staining and flow cytometry. J Antimicrob Chemother 2005;56:678-685.","DOI":"10.1093\/jac\/dki264"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1007\/978-1-60761-611-5_21","volume":"638:","author":"Pina-Vaz C, Rodrigues AG.","year":"2010","journal-title":"Methods Mol Biol"},{"key":"ref=36","doi-asserted-by":"crossref","unstructured":"Pina-Vaz C, Rodrigues AG. Evaluation of antifungal susceptibility using flow cytometry. Methods Mol Biol 2010;638:281-289.","DOI":"10.1007\/978-1-60761-611-5_21"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1099\/00221287-144-2-385","volume":"144:","author":"Helander IM, Latva-Kala K, Lounatmaa K.","year":"1998","journal-title":"Microbiology"},{"key":"ref=38","doi-asserted-by":"crossref","unstructured":"Helander IM, Latva-Kala K, Lounatmaa K. Permeabilizing action of polyethyleneimine on Salmonella typhimurium involves disruption of the outer membrane and interactions with lipopolysaccharide. Microbiology 1998;144:385-390.","DOI":"10.1099\/00221287-144-2-385"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1128\/AAC.01071-07","volume":"52:","author":"Khalil H, Chen T, Riffon R, Wang R, Wang Z.","year":"2008","journal-title":"Antimicrob Agents Chemother"},{"key":"ref=40","doi-asserted-by":"crossref","unstructured":"Khalil H, Chen T, Riffon R, Wang R, Wang Z. Synergy between polyethylenimine and different families of antibiotics against a resistant clinical isolate of Pseudomonas aeruginosa. Antimicrob Agents Chemother 2008;52:1635-1641.","DOI":"10.1128\/AAC.01071-07"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1093\/jac\/43.4.459","volume":"43:","author":"Gould IM.","year":"1999","journal-title":"J Antimicrob Chemother"},{"key":"ref=42","doi-asserted-by":"crossref","unstructured":"Gould IM. A review of the role of antibiotic policies in the control of antibiotic resistance. J Antimicrob Chemother 1999;43:459-465.","DOI":"10.1093\/jac\/43.4.459"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1016\/j.biomaterials.2006.09.026","volume":"28:","author":"Brunot C, Ponsonnet L, Lagneau C, Farge P, Picart C, Grosgogeat B.","year":"2007","journal-title":"Biomaterials"},{"key":"ref=44","doi-asserted-by":"crossref","unstructured":"Brunot C, Ponsonnet L, Lagneau C, Farge P, Picart C, Grosgogeat B. Cytotoxicity of polyethyleneimine (PEI), precursor base layer of polyelectrolyte multilayer films. Biomaterials 2007;28:632-640.","DOI":"10.1016\/j.biomaterials.2006.09.026"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"3995","DOI":"10.1016\/j.biomaterials.2006.03.003","volume":"27:","author":"Beyth N, Yudovin-Farber I, Bahir R, Domb AJ, Weiss EI.","year":"2006","journal-title":"Biomaterials"},{"key":"ref=46","doi-asserted-by":"crossref","unstructured":"Beyth N, Yudovin-Farber I, Bahir R, Domb AJ, Weiss EI. Antibacterial activity of dental composites containing quaternary ammonium polyethylenimine nanoparticles against Streptococcus mutans. Biomaterials 2006;27:3995-4002.","DOI":"10.1016\/j.biomaterials.2006.03.003"}],"container-title":["The Journal of Contemporary Dental Practice"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.thejcdp.com\/doi\/pdf\/10.5005\/jp-journals-10024-1701","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,2]],"date-time":"2021-06-02T06:46:02Z","timestamp":1622616362000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.thejcdp.com\/doi\/10.5005\/jp-journals-10024-1701"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015]]},"references-count":46,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2015]]}},"alternative-id":["10.5005\/jp-journals-10024-1701"],"URL":"https:\/\/doi.org\/10.5005\/jp-journals-10024-1701","relation":{},"ISSN":["1526-3711"],"issn-type":[{"value":"1526-3711","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015]]}}}