{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T03:15:05Z","timestamp":1782962105316,"version":"3.54.5"},"reference-count":0,"publisher":"Termedia Sp. z.o.o.","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arch Med Sci"],"published-print":{"date-parts":[[2021,3,28]]},"abstract":"Introduction<\/jats:title>Parkinson's disease (PD) is a common adult-onset neurodegenerative disorder caused by a progressive loss of dopaminergic neurons due to the accumulation of \u03b1-synuclein in the substantia nigra. Mitochondria are known to play a key role in cell respiratory function and bioenergetics. Indeed, mitochondrial dysfunction causes an insufficient energy production required to satisfy the needs of several organs, especially the nervous system. However, the profiling of messenger RNA (mRNA) expression of mitochondrial subunits in PD has not been systematically investigated yet.<\/jats:p><\/jats:sec>Material and methods<\/jats:title>We explored the mRNA expression of mitochondrial DNA (mtDNA) encoded respiratory chain (RC) subunits in 43 PD patients and 43 normal controls (NC). Next generation sequencing analysis (NGS) and quantitative real-time Polymerase Chain Reaction (qRT-PCR) assay for the confirmation of the NGS results were used.<\/jats:p><\/jats:sec>Results<\/jats:title>All tested mitochondrial RC subunits was significantly over-expressed in subjects with PD compared to NC. In qRT-PCR the mean expression of all mitochondrial subunits had an expression level of at least 7 times compared to NC.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>The over-expression of mitochondrial subunits in PD subjects with respect to NC might be secondary to a degeneration-related alteration of the mitochondrial structure and\/or dynamics, or to the occurrence of a compensatory mechanism. The study of specific mRNA by peripheral blood mononuclear cells may provide a further diagnostic frame to early detect PD patients.<\/jats:p><\/jats:sec>","DOI":"10.5114\/aoms\/131629","type":"journal-article","created":{"date-parts":[[2021,3,28]],"date-time":"2021-03-28T18:03:26Z","timestamp":1616954606000},"source":"Crossref","is-referenced-by-count":7,"title":["mRNA expression profiling of mitochondrial subunits in subjects with Parkinson\u2019s disease"],"prefix":"10.5114","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1075-8993","authenticated-orcid":false,"given":"Michele","family":"Salemi","sequence":"first","affiliation":[{"name":"Oasi Research Institute-IRCCS, Troina. Via Conte Ruggero, 78 \u2013 94018, Troina, Italy, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filomena","family":"Cosentino","sequence":"additional","affiliation":[{"name":"Oasi Research Institute-IRCCS, Troina. Via Conte Ruggero, 78 \u2013 94018, Troina, Italy, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Lanza","sequence":"additional","affiliation":[{"name":"Oasi Research Institute-IRCCS, Troina. Via Conte Ruggero, 78 \u2013 94018, Troina, Italy, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariagiovanna","family":"Cantone","sequence":"additional","affiliation":[{"name":"Department of Neurology, Sant\u2019Elia Hospital, Azienda Sanitaria Provinciale (ASP) Caltanissetta, Via Luigi Russo 6, 93100 Caltanissetta, Italy, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Grazia","family":"Salluzzo","sequence":"additional","affiliation":[{"name":"Oasi Research Institute-IRCCS, Troina. Via Conte Ruggero, 78 \u2013 94018, Troina, Italy, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgio","family":"Giurato","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Medicine and Genomics, Department of Medicine, Surgery and Dentistry \u201cScuola Medica Salernitana\u201d, University of Salerno, 84081 Baronissi (SA), Italy, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugenia","family":"Borgione","sequence":"additional","affiliation":[{"name":"Oasi Research Institute-IRCCS, Troina. Via Conte Ruggero, 78 \u2013 94018, Troina, Italy, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanna","family":"Marchese","sequence":"additional","affiliation":[{"name":"Genomix4Life Srl, Department of Medicine, Surgery and Dentistry \u201cScuola Medica Salernitana\u201d, University of Salerno, Baronissi (SA), Italy, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandro","family":"Santa Paola","sequence":"additional","affiliation":[{"name":"Oasi Research Institute-IRCCS, Troina. Via Conte Ruggero, 78 \u2013 94018, Troina, Italy, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bartolo","family":"Lanuzza","sequence":"additional","affiliation":[{"name":"Oasi Research Institute-IRCCS, Troina. Via Conte Ruggero, 78 \u2013 94018, Troina, Italy, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corrado","family":"Romano","sequence":"additional","affiliation":[{"name":"Oasi Research Institute-IRCCS, Troina. Via Conte Ruggero, 78 \u2013 94018, Troina, Italy, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6937-3065","authenticated-orcid":false,"given":"Raffaele","family":"Ferri","sequence":"additional","affiliation":[{"name":"Oasi Research Institute-IRCCS, Troina. Via Conte Ruggero, 78 \u2013 94018, Troina, Italy, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3061","published-online":{"date-parts":[[2021,3,28]]},"container-title":["Archives of Medical Science"],"original-title":[],"link":[{"URL":"https:\/\/www.archivesofmedicalscience.com\/pdf-131629-63360","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.archivesofmedicalscience.com\/pdf-131629-63360","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,29]],"date-time":"2021-10-29T14:14:57Z","timestamp":1635516897000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.archivesofmedicalscience.com\/mRNA-expression-profiling-of-mitochondrial-subunits-in-subjects-with-Parkinson-s,131629,0,2.html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,28]]},"references-count":0,"alternative-id":["131629"],"URL":"https:\/\/doi.org\/10.5114\/aoms\/131629","relation":{},"ISSN":["1734-1922","1896-9151"],"issn-type":[{"value":"1734-1922","type":"print"},{"value":"1896-9151","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,28]]}}}