{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T05:10:56Z","timestamp":1782537056200,"version":"3.54.5"},"reference-count":19,"publisher":"Termedia Sp. z.o.o.","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arch Med Sci"],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>This study will explore the correlation of peroxisome proliferator\nactivated receptor-\u03b1 (PPAR-\u03b1) regulation of metabolic remodelling in\nthe myocardial fibrosis of atrial fibrillation (AF) in rheumatic heart disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>The left atrial appendage tissues were evaluated\nby Masson staining for fibrosis degree, and Western Blot was used to detect\nthe expression of proteins related to glucose metabolism disorder, lipid metabolism\nabnormality, and mitochondrial dysfunction. The myocardial fibroblasts\nwere established by stimulation with ANG II, and the PPAR-\u03b1 agonist\nGW7647 was administered. The changes of phenotype transformation of\nmyocardial fibroblasts were detected by cellular immunofluorescence, the\nsecretion level of supernatant collagen was detected by ELISA. Finally, the\ncorrelation between PPAR-\u03b1 protein expression and myocardial fibrosis was\nanalysed and a conclusion was drawn.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Masson staining showed that the degree of myocardial fibrosis in\npatients with AF was significantly increased; WB analysis showed that there\nwere statistically significant differences in protein expression related to glucose\nmetabolism disorder, lipid metabolism abnormality, and mitochondrial\ndysfunction. There was a correlation between PPAR-\u03b1 protein expression\nand myocardial fibrosis (&lt;i&gt;r&lt;\/i&gt; = \u20130.5322, &lt;i&gt;p&lt;\/i&gt; &lt; 0.0001). After stimulation with\nPPAR-\u03b1 agonist GW7647, the phenotypic differentiation of myocardial fibro-\nblasts into myofibroblasts was inhibited. The protein expression related\nto mitochondrial dysfunction was statistically different.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study found that there is a negative correlation between\nthe expression of PPAR-\u03b1 protein and myocardial fibrosis in rheumatic heart\ndisease AF, which plays a protective role. PPAR-\u03b1 may participate in the\npathogenesis of myocardial fibrosis in rheumatic heart disease AF by regulating\nglucose metabolism, lipid metabolism, and mitochondrial function.<\/jats:p><\/jats:sec>","DOI":"10.5114\/aoms\/181134","type":"journal-article","created":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T14:42:32Z","timestamp":1732545752000},"page":"1461-1471","source":"Crossref","is-referenced-by-count":5,"title":["PPAR-\u03b1 regulates metabolic remodelling and\nparticipates in myocardial fibrosis in patients with\natrial fibrillation of rheumatic heart disease"],"prefix":"10.5114","volume":"20","author":[{"given":"Xiaoying","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Zigong Fourth People\u2019s Hospital, Zigong, China"},{"name":"Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisong","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5952-3701","authenticated-orcid":true,"given":"Zhenmei","family":"An","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China"},{"name":"Department of Endocrinology and Metabolism , West China Hospital, Sichuan University, Chengdu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3061","published-online":{"date-parts":[[2024,7,24]]},"reference":[{"key":"ref-1225653","doi-asserted-by":"crossref","unstructured":"January CT, Wann LS, Calkins H, et al. 2019 AHA\/ACC\/HRS Focused Update of the 2014 AHA\/ACC\/HRS Guideline for the Management of Patients With Atrial Fibrillation: A Report of the American College of Cardiology\/American Heart Association Task Force on Clinical Practice Guidelines and the Heart Rhythm Society in Collaboration With the Society of Thoracic Surgeons. 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