{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,20]],"date-time":"2025-04-20T22:10:06Z","timestamp":1745187006648,"version":"3.40.4"},"reference-count":0,"publisher":"Termedia Sp. z.o.o.","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arch Med Sci"],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>This study aimed to elucidate the mechanism by which Propofol (PPF) exerts its effects in cerebral ischemia-reperfusion injury (CI\/RI).<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>A rat model of CI\/RI was established via middle cerebral artery occlusion\/reperfusion (MCAO\/R). MCAO\/R rats were pre-treated with PPF (10 mg\/kg) via intraperitoneal injection. Additionally, 48 hours before PPF administration, miR-6838-5p agomir\/antagomir and aquaporin-11 (AQP11) lentiviral overexpression vectors were injected into MCAO\/R rats. Infarct size was determined using 2,3,5-triphenyl tetrazolium chloride staining. Neurological function was assessed using standardized scoring, and cerebral edema was measured by determining brain water content. Hematoxylin-eosin staining, Nissl staining, and Terminal deoxynucleotidyl transferase dUTP nick end labeling staining were performed on brain tissues. Inflammatory and oxidative markers were evaluated. A hypoxia\/reoxygenation (H\/R) injury model was established in PC12 cells to assess miR-6838-5p and AQP11 expression levels, as well as cell viability and apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The lethal dose 50 (LD\u2085\u2080) of PPF in rats was determined to be 22.6 mg\/kg, significantly higher than the therapeutic dose. PPF or overexpression of miR-6838-5p resulted in reduced cerebral infarct size, neuronal necrosis, and apoptosis, increased Nissl bodies, and decreased brain edema, apoptosis, tumor necrosis factor-\u03b1, interleukin-1\u03b2, and malondialdehyde (MDA) levels. Glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities were elevated in MCAO\/R rats treated with PPF. These protective effects of PPF were reversed by miR-6838-5p knockdown or AQP11 overexpression. PPF ameliorated H\/R-induced neuronal damage, enhanced neuronal activity, reduced apoptosis and MDA production, and increased GSH-Px and SOD levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>PPF ameliorates CI\/RI by modulating the miR-6838-5p\/AQP11 axis.<\/jats:p><\/jats:sec>","DOI":"10.5114\/aoms\/201185","type":"journal-article","created":{"date-parts":[[2025,4,20]],"date-time":"2025-04-20T21:36:16Z","timestamp":1745184976000},"source":"Crossref","is-referenced-by-count":0,"title":["Propofol improves neuronal apoptosis and oxidative damage mediated by cerebral ischemia-reperfusion by regulating the miR-6838-5p\/AQP11 axis"],"prefix":"10.5114","author":[{"given":"XiuQin","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Anesthesiology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, The 960th Hospital of the PLA, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YangYang","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YongLai","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3061","published-online":{"date-parts":[[2025,4,20]]},"container-title":["Archives of Medical Science"],"original-title":[],"link":[{"URL":"https:\/\/www.archivesofmedicalscience.com\/pdf-201185-124636","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.archivesofmedicalscience.com\/pdf-201185-124636","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,20]],"date-time":"2025-04-20T21:36:16Z","timestamp":1745184976000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.archivesofmedicalscience.com\/Propofol-improves-neuronal-apoptosis-and-oxidative-damage-mediated-by-cerebral-ischemia,201185,0,2.html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,20]]},"references-count":0,"alternative-id":["201185"],"URL":"https:\/\/doi.org\/10.5114\/aoms\/201185","relation":{},"ISSN":["1734-1922","1896-9151"],"issn-type":[{"value":"1734-1922","type":"print"},{"value":"1896-9151","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,20]]}}}