{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T07:49:34Z","timestamp":1775202574402,"version":"3.50.1"},"reference-count":0,"publisher":"Termedia Sp. z.o.o.","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arch Med Sci"],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Disturbed mitochondrial activity in adipocytes is suggested as one of the mechanisms involved in metabolic dysfunction in obesity. Glucagon-like peptide receptor agonists (GLP-1RAs) are used to normalize glucose level and reduce body weight. GLP-1 activates similar intracellular pathway as irisin, peptide that modulate metabolism by stimulating the \u2018browning\u2019 of adipocytes. The aim of the study was to investigate the mechanisms of the GLP-1RA, exendin-4, action at the level of mRNA, proteins and mitochondrial activity in human adipocytes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material and methods<\/jats:title>\n                    <jats:p>The human preadipocytes Chub-S7 were differentiated in vitro to mature adipocytes and then stimulated with exendin-4 at 100 nM for 24 h. Expression of mRNA and proteins (irisin, adiponectin, visfatin\/NAMPT) were measured. Oxygen consumption rates and intracellular ATP content were determined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The exendin-4 enhanced the secretion of irisin and visfatin by adipocytes. The up-regulated expression of FNDC5, NAMPT and UCP2 genes was accompanied by modest changes in mitochondrial activity in exendin-4 treated adipocytes. Exendin-4 exerted similar effect on mitochondrial oxygen consumption rates as irisin, including increased maximum mitochondrial respiration and reserve capacity with unchanged intracellular ATP.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Increasing energy expenditure by exendin-4 may be associated with upregulation of irisin in human adipocytes. Clinical studies are necessary to confirm the hypothesis that nutrients, by stimulating the secretion of GLP-1, may influence the expression of irisin and thus modulate the mitochondrial metabolism of adipocytes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.5114\/aoms\/214730","type":"journal-article","created":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T05:39:51Z","timestamp":1775194791000},"source":"Crossref","is-referenced-by-count":0,"title":["Irisin may be involved in exendin-4 mitochondrial action in human adipocytes"],"prefix":"10.5114","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2849-5119","authenticated-orcid":true,"given":"Agnieszka","family":"Dziewo\u0144ska","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry, Jagiellonian University Medical College, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8142-0012","authenticated-orcid":true,"given":"Anna","family":"Polus","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, Jagiellonian University Medical College, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6351-854X","authenticated-orcid":true,"given":"Anna","family":"Gruca","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, Jagiellonian University Medical College, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0121-8154","authenticated-orcid":true,"given":"Bogdan","family":"Solnica","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, Jagiellonian University Medical College, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9557-8440","authenticated-orcid":true,"given":"Joanna","family":"G\u00f3ralska","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, Jagiellonian University Medical College, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3061","published-online":{"date-parts":[[2026,4,3]]},"container-title":["Archives of Medical Science"],"original-title":[],"link":[{"URL":"https:\/\/www.archivesofmedicalscience.com\/pdf-214730-138227","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.archivesofmedicalscience.com\/pdf-214730-138227","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T05:39:53Z","timestamp":1775194793000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.archivesofmedicalscience.com\/Irisin-may-be-involved-in-exendin-4-mitochondrial-action-in-human-adipocytes,214730,0,2.html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,3]]},"references-count":0,"alternative-id":["214730"],"URL":"https:\/\/doi.org\/10.5114\/aoms\/214730","relation":{},"ISSN":["1734-1922","1896-9151"],"issn-type":[{"value":"1734-1922","type":"print"},{"value":"1896-9151","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,3]]}}}