{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T10:27:38Z","timestamp":1771669658843,"version":"3.50.1"},"reference-count":0,"publisher":"Termedia Sp. z.o.o.","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arch Med Sci"],"published-print":{"date-parts":[[2019,11,11]]},"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Previous studies have reported that microRNAs are implicated in the pathogenesis of diabetic nephropathy (DN). In this study, the underlying molecular mechanisms and diagnostic significance of miR-372-3p were investigated in the process of DN.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>Cell proliferation and apoptosis were measured using MTT and Annexin V-FITC double staining, respectively. RT-qPCR and western blotting were used to measure the expression levels of mRNA and protein. The diagnostic power of miR-372-3p in plasma for DN was evaluated using the receiver operating characteristics (ROC) curves and the area under the ROC curves (AUC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>miR microarray analysis revealed that 126 miRs were significantly differentially expressed in response to high glucose stimulation. Among these miRs, high glucose stimulated miR-372-3p expression at the highest level. In vitro experimental measurements showed that knockdown of miR-372-3p showed the ability to reverse high glucose-induced glomerular endothelial cell apoptosis and impairment of eNOS\/NO bioactivity. Mechanistic analysis revealed that fibroblast growth factor-16 (FGF-16) as a direct of miR-372-3p protected against high glucose-induced glomerular endothelial cell dysfunction. ROC analysis revealed that the diagnostic value of miR-372-3p, miR-15a or miR-372-3p combined with miR-15a in type 2 diabetes mellitus patients (AUC = 0.841, p &lt; 0.001; AUC = 0.822, p &lt; 0.001 or AUC = 0.922, p &lt; 0.001) with DN was better than in type 1 diabetes mellitus patients (AUC = 0.805, p &lt; 0.001; AUC = 0.722, p &lt; 0.001 or AUC = 0.865, p &lt; 0.001) with DN.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>miR-372-3p might be a valuable therapeutic target and diagnostic marker for patients with DN.<\/jats:p><\/jats:sec>","DOI":"10.5114\/aoms.2019.89659","type":"journal-article","created":{"date-parts":[[2019,11,20]],"date-time":"2019-11-20T10:09:59Z","timestamp":1574244599000},"source":"Crossref","is-referenced-by-count":2,"title":["miR-372-3p is a potential diagnostic factor for diabetic nephropathy and modulates high glucose-induced glomerular endothelial cell dysfunction via targeting fibroblast growth factor-16"],"prefix":"10.5114","author":[{"given":"Zhiyun","family":"Meng","sequence":"first","affiliation":[{"name":"Department of Nephrology, Weifang City Traditional Chinese Medical Hospital, Weifang, Shandong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fangyuan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Weifang City Traditional Chinese Medical Hospital, Weifang, Shandong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Weifang City Traditional Chinese Medical Hospital, Weifang, Shandong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3061","published-online":{"date-parts":[[2019,11,11]]},"container-title":["Archives of Medical Science"],"original-title":[],"link":[{"URL":"https:\/\/www.archivesofmedicalscience.com\/pdf-112198-59756","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.archivesofmedicalscience.com\/pdf-112198-59756","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,29]],"date-time":"2021-10-29T14:21:50Z","timestamp":1635517310000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.archivesofmedicalscience.com\/miR-372-3p-is-a-potential-diagnostic-factor-for-diabetic-nephropathy-and-modulates,112198,0,2.html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,11]]},"references-count":0,"alternative-id":["112198"],"URL":"https:\/\/doi.org\/10.5114\/aoms.2019.89659","relation":{},"ISSN":["1734-1922","1896-9151"],"issn-type":[{"value":"1734-1922","type":"print"},{"value":"1896-9151","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,11,11]]}}}