{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,27]],"date-time":"2024-07-27T13:20:23Z","timestamp":1722086423034},"reference-count":0,"publisher":"Termedia Sp. z.o.o.","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arch Med Sci"],"published-print":{"date-parts":[[2020,11,25]]},"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>The purpose of this study was to investigate long non-coding RNA (lncRNA) maternally expressed gene 3 (MEG3) effects in vitamin D (Vit. D) treatment in endothelial cell damage induced by diabetes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>We used human umbilical vein endothelial cells (HUVECs) as a research objective in our study and used high glucose in a diabetic cell model. We evaluated cell apoptosis by flow cytometry, inflammatory factors (IL-6, IL-1\u03b2 and TNF-\u03b1) concentrations by ELISA assay, relative gene and protein expression by RT-qPCR and WB assay, and NF-\u03baB(p65) nuclear volume by cellular immunofluorescence.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared with the NC (normal control) group, the cell apoptosis rate was significantly increased, inflammatory factor (IL-6, IL-1\u03b2 and TNF-\u03b1) concentrations were significantly up-regulated, lncRNA MEG3 gene expression was significantly depressed, Toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88) and nuclear factor \uf06bB p65 (NF-\u03baB(p65)) gene and protein expression levels were significantly increased and NF-\u03baB(p65) nuclear volume was significantly up-regulated (p &lt; 0.001, respectively). With Vit. D supplementation, compared with the Model group, Vit. D improved endothelial cell damage induced by diabetes, while lncRNA MEG3 was significantly increased and the TLR4\/MyD88\/NF-\u03baB(p65) pathway was significantly depressed dose-dependently (all p &lt; 0.05). With sh-MEG3 transfection, the Vit. D treatment effects were significantly reduced.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Vit. D improved endothelial cell damage induced by diabetes via lncRNA MEG3 up-regulation in vitro study.<\/jats:p><\/jats:sec>","DOI":"10.5114\/aoms.2020.101248","type":"journal-article","created":{"date-parts":[[2021,3,3]],"date-time":"2021-03-03T09:25:45Z","timestamp":1614763545000},"source":"Crossref","is-referenced-by-count":0,"title":["Vitamin D ameliorated endothelial cell damage induced by diabetes via regulation of lncRNA MEG3 in human umbilical vein endothelial cells"],"prefix":"10.5114","author":[{"given":"Hua","family":"Wei","sequence":"first","affiliation":[{"name":"Department of General Medicine, Affiliated Hospital of YouJiang Medical University for Nationalities, Baise, Guangxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiongfang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of General Medicine, The second NanNing people\u2019s hospital, NanNing Guangxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of General Medicine, Affiliated Hospital of YouJiang Medical University for Nationalities, Baise, Guangxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Yang","sequence":"additional","affiliation":[{"name":"Health Supervision Center, Affiliated Hospital of YouJiang Medical University for Nationalities, Baise, Guangxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of General Medicine, Affiliated Hospital of YouJiang Medical University for Nationalities, Baise, Guangxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun-chun","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of General Medicine, Affiliated Hospital of YouJiang Medical University for Nationalities, Baise, Guangxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying-chuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Graduate School, YouJiang Medical University for Nationalities, Baise, Guangxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing-qian","family":"Hu","sequence":"additional","affiliation":[{"name":"Graduate School, YouJiang Medical University for Nationalities, Baise, Guangxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xi","family":"Wei","sequence":"additional","affiliation":[{"name":"Health Supervision Center, Affiliated Hospital of YouJiang Medical University for Nationalities, Baise, Guangxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3061","published-online":{"date-parts":[[2020,11,25]]},"container-title":["Archives of Medical Science"],"original-title":[],"link":[{"URL":"https:\/\/www.archivesofmedicalscience.com\/pdf-128013-59455","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.archivesofmedicalscience.com\/pdf-128013-59455","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,29]],"date-time":"2021-10-29T14:15:55Z","timestamp":1635516955000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.archivesofmedicalscience.com\/Vitamin-D-ameliorated-endothelial-cell-damage-induced-by-diabetes-via-regulation,128013,0,2.html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,25]]},"references-count":0,"alternative-id":["128013"],"URL":"https:\/\/doi.org\/10.5114\/aoms.2020.101248","relation":{},"ISSN":["1734-1922","1896-9151"],"issn-type":[{"value":"1734-1922","type":"print"},{"value":"1896-9151","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,25]]}}}