{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T08:36:03Z","timestamp":1768638963266,"version":"3.49.0"},"reference-count":0,"publisher":"Termedia Sp. z.o.o.","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arch Med Sci"],"published-print":{"date-parts":[[2020,2,24]]},"abstract":"Introduction<\/jats:title>The function of the C6orf120 gene, which encodes an N-glycosylated protein, remains unknown. The study was performed to characterize the utility of the C6orf120 gene in carbon tetrachloride-induced acute liver injury and to elucidate the potential underlying mechanisms by establishing a C6orf120 gene-knockout (C6orf120<sup>\u2013\/\u2013<\/sup>) rat model.<\/jats:p><\/jats:sec>Material and methods<\/jats:title>C6orf120<sup>-\/-<\/sup> and wild-type (WT) rats were intraperitoneally administered with CCl<sub>4<\/sub> (1 : 1 v\/v in olive oil, 2 \u00b5l\/g). Rats were sacrificed 24 h after CCl<sub>4<\/sub> administration. Liver tissues were collected for H&E, IHC, qRT-PCR, and Western blot analysis.<\/jats:p><\/jats:sec>Results<\/jats:title>C6orf120 gene deficiency may be vulnerable to CCl<sub>4<\/sub>-induced acute liver injury in rats as indicated by the high levels of alanine aminotransferase (WT: 388.7 \u00b155.96 vs. C6orf120<sup>\u2013\/\u2013<\/sup>: 915.9 \u00b1118.8, p < 0.001) and greater degree of pathological damage. Quantitative reverse transcription polymerase chain reaction showed that the mRNA levels of inflammation-associated cytokines, such as interleukin (IL)-1\u03b2, IL-6, and tumor necrosis factor (TNF)-\uf061, in liver tissues were increased in C6orf120<sup>\u2013\/\u2013<\/sup> rats compared with those in WT rats. Moreover, western blot showed that the protein expression of cytokines nucleotide-binding oligomerization domain leucine rich repeat and pyrin domain containing 3 (NLRP3), caspase-1, IL-1\u03b2, nuclear factor-\u03baB, c-Jun N-terminal kinases, and Bax were increased in C6orf120<sup>\u2013\/\u2013<\/sup> rats compared with those in WT rats.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>C6orf120<sup>\u2013\/\u2013<\/sup> rats were susceptible to CCl<sub>4<\/sub>-induced liver injury, which may be related to NLRP3 inflammasome and JNK signaling pathway activation.<\/jats:p><\/jats:sec>","DOI":"10.5114\/aoms.2020.93214","type":"journal-article","created":{"date-parts":[[2020,2,25]],"date-time":"2020-02-25T14:02:36Z","timestamp":1582639356000},"source":"Crossref","is-referenced-by-count":2,"title":["C6orf120 gene deficiency may be vulnerable to carbon tetrachloride induced acute hepatic injury in rats"],"prefix":"10.5114","author":[{"given":"Jian","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Center of Integrated Traditional Chinese and Western Medicine, Peking University Ditan Teaching Hospital, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Man-ka","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Center of Integrated Traditional Chinese and Western Medicine, Peking University Ditan Teaching Hospital, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui-min","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Center of Integrated Traditional Chinese and Western Medicine, Beijing Ditan Hospital, Capital Medical University, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin-cheng","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Center of Integrated Traditional Chinese and Western Medicine, Peking University Ditan Teaching Hospital, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan-ni","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Center of Integrated Traditional Chinese and Western Medicine, Peking University Ditan Teaching Hospital, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Center of Integrated Traditional Chinese and Western Medicine, Beijing Ditan Hospital, Capital Medical University, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling-ling","family":"He","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Beijing Ditan Hospital, Capital Medical University, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-hui","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Peking University Ditan Teaching Hospital, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei-xin","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Peking University Ditan Teaching Hospital, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Center of Integrated Traditional Chinese and Western Medicine, Peking University Ditan Teaching Hospital, Beijing, China"},{"name":"Department of Center of Integrated Traditional Chinese and Western Medicine, Beijing Ditan Hospital, Capital Medical University, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3061","published-online":{"date-parts":[[2020,2,24]]},"container-title":["Archives of Medical Science"],"original-title":[],"link":[{"URL":"https:\/\/www.archivesofmedicalscience.com\/pdf-113029-59677","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.archivesofmedicalscience.com\/pdf-113029-59677","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,29]],"date-time":"2021-10-29T14:21:13Z","timestamp":1635517273000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.archivesofmedicalscience.com\/C6orf120-gene-deficiency-may-be-vulnerable-to-carbon-tetrachloride-induced-acute,113029,0,2.html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,24]]},"references-count":0,"alternative-id":["113029"],"URL":"https:\/\/doi.org\/10.5114\/aoms.2020.93214","relation":{},"ISSN":["1734-1922","1896-9151"],"issn-type":[{"value":"1734-1922","type":"print"},{"value":"1896-9151","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,24]]}}}