{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,5]],"date-time":"2022-04-05T07:48:53Z","timestamp":1649144933211},"reference-count":0,"publisher":"Termedia Sp. z.o.o.","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arch Med Sci"],"published-print":{"date-parts":[[2020,5,7]]},"abstract":"Introduction<\/jats:title>The aim was to observe the effect of Toll-like receptor 4 (TLR4) deficiency on clinical severity and expression of Th1\/Th2\/Th17-associated cytokines in experimental autoimmune neuritis (EAN).<\/jats:p><\/jats:sec>Material and methods<\/jats:title>We selected C57BL\/10 wild type (WT) mice and TLR4 knockout (KO) mice with the C57BL\/10 background for induction of the EAN model by immunizing mice twice (days 0 and 8) via subcutaneous injection of 180 \u00b5g P0 peptide 180\u2013199 emulsion in 25 \u00b5l of PBS and 0.5 mg Mycobacterium tuberculosis (Difco, USA) in 25 \u00b5l of Freund\u2019s incomplete adjuvant into the back of mice. The concentrations of serum cytokines (IL-2, IL-4, IL-6, IL-10, IL-17A, IFN-\u03b3 and TNF) were determined using the Ms Th1\/Th2\/Th17 CBA kit.<\/jats:p><\/jats:sec>Results<\/jats:title>We found that TLR4 deficiency could attenuate the clinical severity and delay the onset of EAN. Moreover, our data showed that the sera levels of IFN-\u03b3, TNF, IL-6 and IL-17A were elevated in the WT mice with EAN when compared with the naive WT mice, but only the production of IL-17A was significantly lower in the TLR4 KO mice with EAN than in their WT counterparts.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Based on these findings, TLR4 may contribute to the pathogenesis of EAN by regulating Th17 cells and the production of Th17-associated factors. However, the exact mechanism remains unclear and more evidence is needed to elucidate its role in EAN.<\/jats:p><\/jats:sec>","DOI":"10.5114\/aoms.2020.94982","type":"journal-article","created":{"date-parts":[[2020,5,22]],"date-time":"2020-05-22T14:10:20Z","timestamp":1590156620000},"source":"Crossref","is-referenced-by-count":0,"title":["Effect of Toll-like receptor 4 deficiency on clinical severity and expression of Th1\/Th2\/Th17-associated cytokines in a murine model of experimental autoimmune neuritis"],"prefix":"10.5114","author":[{"given":"Li-Juan","family":"Wang","sequence":"first","affiliation":[{"name":"Laboratory Diagnosis Center, Beijing Tiantan Hospital, Capital Medical University, Beijing, China"},{"name":"China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, China"},{"name":"Monogenic Disease Research Center for Neurological Disorder, Beijing, China"},{"name":"Precision Medicine Research Center for Neurological Disorder, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, Care Sciences and Society, Karolinska Institute, Stockholm, Sweden"},{"name":"Neuroscience Center, Department of Neurology, the First Hospital of Jilin University, Changchun, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiu-Juan","family":"Wu","sequence":"additional","affiliation":[{"name":"Neuroscience Center, Department of Neurology, the First Hospital of Jilin University, Changchun, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Li","sequence":"additional","affiliation":[{"name":"Neuroscience Center, Department of Neurology, the First Hospital of Jilin University, Changchun, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun-Jiao","family":"Yang","sequence":"additional","affiliation":[{"name":"Laboratory Diagnosis Center, Beijing Tiantan Hospital, Capital Medical University, Beijing, China"},{"name":"China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, China"},{"name":"Monogenic Disease Research Center for Neurological Disorder, Beijing, China"},{"name":"Precision Medicine Research Center for Neurological Disorder, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xi-Xiong","family":"Kang","sequence":"additional","affiliation":[{"name":"Laboratory Diagnosis Center, Beijing Tiantan Hospital, Capital Medical University, Beijing, China"},{"name":"China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, China"},{"name":"Monogenic Disease Research Center for Neurological Disorder, Beijing, China"},{"name":"Precision Medicine Research Center for Neurological Disorder, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong-Liang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Neuroscience Center, Department of Neurology, the First Hospital of Jilin University, Changchun, China"},{"name":"Department of Life Sciences, the National Natural Science Foundation of China, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo-Jun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Laboratory Diagnosis Center, Beijing Tiantan Hospital, Capital Medical University, Beijing, China"},{"name":"China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, China"},{"name":"Monogenic Disease Research Center for Neurological Disorder, Beijing, China"},{"name":"Precision Medicine Research Center for Neurological Disorder, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3061","published-online":{"date-parts":[[2020,5,7]]},"container-title":["Archives of Medical Science"],"original-title":[],"link":[{"URL":"https:\/\/www.archivesofmedicalscience.com\/pdf-113026-59628","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.archivesofmedicalscience.com\/pdf-113026-59628","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,29]],"date-time":"2021-10-29T14:21:11Z","timestamp":1635517271000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.archivesofmedicalscience.com\/Effect-of-Toll-like-receptor-4-deficiency-on-clinical-severity-and-expression-of,113026,0,2.html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,7]]},"references-count":0,"alternative-id":["113026"],"URL":"https:\/\/doi.org\/10.5114\/aoms.2020.94982","relation":{},"ISSN":["1734-1922","1896-9151"],"issn-type":[{"value":"1734-1922","type":"print"},{"value":"1896-9151","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,7]]}}}