{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,3]],"date-time":"2022-04-03T19:09:38Z","timestamp":1649012978191},"reference-count":0,"publisher":"Termedia Sp. z.o.o.","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arch Med Sci"],"published-print":{"date-parts":[[2020,5,25]]},"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>The aim of this work is to investigate the inhibitory effect of aloperin (Alo) on hepatocyte apoptosis in non-alcoholic fatty liver disease, and the underlying mechanism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>Rats in the Alo groups were fed a\u00a0high-fat + high-sugar diet for eight weeks and then treated with low-, moderate-, and high-dose Alo for another eight weeks via gavage. Oxidative stress indices were tested by a\u00a0colourimetric method, and pathological changes were observed by haematoxylin\u2013eosin staining. Apoptosis was detected by TUNEL staining. TLR4, TRIF, and NF-\uf06bB(p65) mRNA and protein expressions were detected by RT-qPCR, Western blot assay and immunohistochemistry. In the in vitro study, L02 cells were treated with FFA (free fatty acid) for 24 h to establish a\u00a0non-alcoholic steatohepatitis (NASH) model. Inhibition of cell proliferation was measured by the MTT method, and cell apoptosis was evaluated by flow cytometry. Finally, the nuclear import volume of NF-\uf06bB(p65) was evaluated by cellular immunofluorescence.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Cell apoptosis significantly decreased in the Alo-treatment groups in a\u00a0dose-dependent manner (p &lt; 0.05). TLR4, TRIF, and NF-\uf06bB(p65) expression in the Alo-treatment groups was significantly downregulated compared with model group (p &lt; 0.05). The cell proliferation rate significantly increased, cell apoptosis significantly decreased (p &lt; 0.05), and the TLR4\/TRIF\/NF-\uf06bB pathway was significantly inhibited (p &lt; 0.05) in the Alo-treatment groups. The nuclear import volume of NF-\uf06bB(p65) in the Alo-treatment groups was significantly decreased compared with that in the model group in a\u00a0dose-dependent manger (p &lt; 0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Alo could improve NASH via the TLR4\/TRIF\/NF-\uf06bB pathway.<\/jats:p><\/jats:sec>","DOI":"10.5114\/aoms.2020.95629","type":"journal-article","created":{"date-parts":[[2020,6,15]],"date-time":"2020-06-15T09:30:38Z","timestamp":1592213438000},"source":"Crossref","is-referenced-by-count":0,"title":["Aloperin improves liver non-alcoholic steatohepatitis \nin vitro and in vivo"],"prefix":"10.5114","author":[{"given":"Zhongsheng","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Infection Diseases and Liver Diseases, The First Affiliated Hospital of Shandong First Medical University (Shandong Provincial Qianfoshan Hospital), Jinan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruyu","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Hepatology, Beijing Ditan Hospital Capital Medical University, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liwei","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Infection Diseases and Liver Diseases, The First Affiliated Hospital of Shandong First Medical University (Shandong Provincial Qianfoshan Hospital), Jinan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sumei","family":"Lu","sequence":"additional","affiliation":[{"name":"PCR laboratory,The First Affiliated Hospital of Shandong First Medical University (Shandong Provincial Qianfoshan Hospital), Jinan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, The First Affiliated Hospital of Shandong First Medical University (Shandong Provincial Qianfoshan Hospital), Jinan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaofei","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Infection Diseases and Liver Diseases, The First Affiliated Hospital of Shandong First Medical University (Shandong Provincial Qianfoshan Hospital), Jinan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"An","sequence":"additional","affiliation":[{"name":"Department of Infection Diseases and Liver Diseases, The First Affiliated Hospital of Shandong First Medical University (Shandong Provincial Qianfoshan Hospital), Jinan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3061","published-online":{"date-parts":[[2020,5,25]]},"container-title":["Archives of Medical Science"],"original-title":[],"link":[{"URL":"https:\/\/www.archivesofmedicalscience.com\/pdf-118004-59619","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.archivesofmedicalscience.com\/pdf-118004-59619","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,29]],"date-time":"2021-10-29T14:19:37Z","timestamp":1635517177000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.archivesofmedicalscience.com\/Aloperin-improves-liver-non-alcoholic-steatohepatitis-r-nin-vitro-and-in-vivo,118004,0,2.html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,25]]},"references-count":0,"alternative-id":["118004"],"URL":"https:\/\/doi.org\/10.5114\/aoms.2020.95629","relation":{},"ISSN":["1734-1922","1896-9151"],"issn-type":[{"value":"1734-1922","type":"print"},{"value":"1896-9151","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,25]]}}}