{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,4]],"date-time":"2022-04-04T10:58:10Z","timestamp":1649069890712},"reference-count":0,"publisher":"Termedia Sp. z.o.o.","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arch Med Sci"],"published-print":{"date-parts":[[2020,7,17]]},"abstract":"Introduction<\/jats:title>Methotrexate (MTX) is the treatment of choice for patients\nwith rheumatoid arthritis (RA). However, it has been found to produce toxicity in some patients. Different polymorphisms can play a part in inter-individual differences in toxicity. Our aim is therefore to determine the influence of gene polymorphisms in the MTX metabolic pathway, such as MTHFR\n(rs1801133 and rs1801131), MTHFD1 (rs2236225), MTR (rs1805087), and\nABCC2 (rs4148396), on toxicity in MTX treatment among Caucasian patients\ndiagnosed with RA.<\/jats:p><\/jats:sec>Material and methods<\/jats:title>Real-time polymnerase chain reaction (PCR) analysis\nwith TaqMan probes was performed on these polymorphisms in 200 patients in a retrospective study.<\/jats:p><\/jats:sec>Results<\/jats:title>Patients with TT genotype and C allele for MTHFR rs1801133 gene\npolymorphism presented a higher risk of anaemia (p = 0.0304; OR = 3.70;\n95% CI: 1.10\u201312.34) and dizziness (p = 0.0438; OR = 8.15; 95% CI: 1.61\u2013\n148.68). Patients with MTHFR rs1801131-C allele or CC genotype presented a higher risk of mucositis (p = 0.0188; OR = 3.02; 95% CI: 1.22\u20137.91),\nacneiform rash (p = 0.0322; OR = 5.74; 95% CI: 1.34\u201339.21), and alopecia\n(p = 0.0072; OR = 5.11; 95% CI: 1.37\u201317.70). Patients with MTR rs1805087-\nCC genotype presented a higher risk of anosmia (p = 0.0038; OR = 98.00;\n95% CI: 3.16\u2013infinite); patients with the TT genotype or T allele for MTHFD1\nrs2236225 presented a higher risk of liver failure (p = 0.00229; OR = 2.34;\n95% CI: 1.14\u20134.96) and alopecia (p = 0.0248; OR = 3.83; 95% CI: 1.10\u201313.30),\nand patients with the TT genotype for ABCC2 rs4148396 polymorphism (p =\n0.0466; OR = 2.67; 95% CI: 0.98\u20136.94) presented a higher risk of headaches.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Pharmacogenomic analysis of these polymorphisms may facilitate decision-making in relation to MTX treatment toxicity among RA patients.<\/jats:p><\/jats:sec>","DOI":"10.5114\/aoms.2020.97360","type":"journal-article","created":{"date-parts":[[2020,7,26]],"date-time":"2020-07-26T21:06:57Z","timestamp":1595797617000},"source":"Crossref","is-referenced-by-count":0,"title":["Association of genetic polymorphisms on methotrexate toxicity in patients with rheumatoid arthritis"],"prefix":"10.5114","author":[{"given":"Mar\u00eda","family":"Zarzuelo Romero","sequence":"first","affiliation":[{"name":"Department of Pharmacy and Pharmaceutical Technology, Social and Legal Assistance Pharmacy Section, Faculty of Pharmacy, University of Granada, Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Maldonado Montoro","sequence":"additional","affiliation":[{"name":"Clinical Analysis Service, Hospital Campus de la Salud, Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Pharmacy Service, Pharmacogenetics Unit, University Hospital Virgen Macarena, Sevilla, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noelia","family":"Pete","sequence":"additional","affiliation":[{"name":"Pharmacy Service, Pharmacogenetics Unit, University Hospital Virgen de las Nieves, UGC Provincial de Farmacia de Granada, Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Almudena","family":"Mart\u00edn","sequence":"additional","affiliation":[{"name":"Pharmacy Service, Pharmacogenetics Unit, University Hospital Virgen de las Nieves, UGC Provincial de Farmacia de Granada, Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Department of Pharmacy and Pharmaceutical Technology, Social and Legal Assistance Pharmacy Section, Faculty of Pharmacy, University of Granada, Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel","family":"Calleja Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Pharmacy Service, Pharmacogenetics Unit, University Hospital Virgen Macarena, Sevilla, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Ram\u00edrez Tortosa","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy, University of Granada, Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Morales","sequence":"additional","affiliation":[{"name":"Pharmacy Service, Pharmacogenetics Unit, University Hospital Virgen de las Nieves, UGC Provincial de Farmacia de Granada, Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3061","published-online":{"date-parts":[[2020,7,17]]},"container-title":["Archives of Medical Science"],"original-title":[],"link":[{"URL":"https:\/\/www.archivesofmedicalscience.com\/pdf-123790-62997","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.archivesofmedicalscience.com\/pdf-123790-62997","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,29]],"date-time":"2021-10-29T14:17:44Z","timestamp":1635517064000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.archivesofmedicalscience.com\/Association-of-genetic-polymorphisms-on-methotrexate-toxicity-in-patients-with-rheumatoid,123790,0,2.html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,17]]},"references-count":0,"alternative-id":["123790"],"URL":"https:\/\/doi.org\/10.5114\/aoms.2020.97360","relation":{},"ISSN":["1734-1922","1896-9151"],"issn-type":[{"value":"1734-1922","type":"print"},{"value":"1896-9151","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,17]]}}}