{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,4]],"date-time":"2022-04-04T21:46:38Z","timestamp":1649108798038},"reference-count":0,"publisher":"Termedia Sp. z.o.o.","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arch Med Sci"],"published-print":{"date-parts":[[2020,9,7]]},"abstract":"Introduction<\/jats:title>Uterine leiomyomas (UL) are highly prevalent benign smooth\n\nmuscle tumors, seen in approximately 70% of women. These hormone re-\nsponsive tumors are also known to secrete prolactin (PRL), a hormone\n\nof the anterior pituitary gland. Elevated levels of serum prolactin are a com-\nmon clinical finding in different gynecological pathologies including UL.\n\nHowever, the underlying causes for this elevation are not yet clear. Therefore,\nthe main objective of this study is to measure the serum PRL in UL patients\nand also to investigate its molecular connection with coding region somatic\nmutations of the PRL gene.<\/jats:p><\/jats:sec>Material and methods<\/jats:title>The serum PRL levels of UL patients were measured\nthrough the ELISA method. The coding region PRL gene mutations in UL\nand corresponding myometrium tissues were screened through the Sanger\nsequencing method.<\/jats:p><\/jats:sec>Results<\/jats:title>Uterine leiomyoma patients demonstrated significant elevation\n\nof the PRL hormone level in serum samples (p \u2264 0.01). No somatic coding re-\ngion mutations in the PRL gene were identified. However, four germline vari-\nants (c.570G>A, c.205-102T>A, c.312+177T>C and c.269C>T) were detected.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>This study is the first one to confirm that serum PRL level ele-\nvation among UL patients is not connected to somatic mutations in the PRL\n\ngene. However, PRL genetic polymorphisms may indirectly contribute to the\ndisease etiology.<\/jats:p><\/jats:sec>","DOI":"10.5114\/aoms.2020.98658","type":"journal-article","created":{"date-parts":[[2020,9,16]],"date-time":"2020-09-16T08:29:05Z","timestamp":1600244945000},"source":"Crossref","is-referenced-by-count":2,"title":["Assessing the\u00a0role of\u00a0serum prolactin levels and coding region somatic mutations of\u00a0the\u00a0prolactin gene in Saudi uterine leiomyoma patients"],"prefix":"10.5114","author":[{"given":"Hanan Abdelhalim","family":"ElSokary","sequence":"first","affiliation":[{"name":"Department of Genetic Medicine, Faculty of Medicine, King Abdulaziz University,\nJeddah, Saudi Arabia"},{"name":"Princess Al-Jawhara Al-Brahim Center of Excellence in Research of Hereditary\nDisorders, King Abdulaziz University, Jeddah, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Layla Saleh","family":"Abdullah","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Medicine, King Abdulaziz University, Jeddah,\nSaudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amani","family":"Ujaimi","sequence":"additional","affiliation":[{"name":"Department of Genetic Medicine, Faculty of Medicine, King Abdulaziz University,\nJeddah, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nora Naif","family":"Sahly","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Medicine King Abdulaziz\nUniversity, Jeddah, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nisma","family":"Mansouri","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Medicine King Abdulaziz\nUniversity, Jeddah, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Babajan","family":"Banaganapalli","sequence":"additional","affiliation":[{"name":"Department of Genetic Medicine, Faculty of Medicine, King Abdulaziz University,\nJeddah, Saudi Arabia"},{"name":"Princess Al-Jawhara Al-Brahim Center of Excellence in Research of Hereditary\nDisorders, King Abdulaziz University, Jeddah, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noor Ahmad","family":"Shaik","sequence":"additional","affiliation":[{"name":"Department of Genetic Medicine, Faculty of Medicine, King Abdulaziz University,\nJeddah, Saudi Arabia"},{"name":"Princess Al-Jawhara Al-Brahim Center of Excellence in Research of Hereditary\nDisorders, King Abdulaziz University, Jeddah, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramu","family":"Elango","sequence":"additional","affiliation":[{"name":"Department of Genetic Medicine, Faculty of Medicine, King Abdulaziz University,\nJeddah, Saudi Arabia"},{"name":"Princess Al-Jawhara Al-Brahim Center of Excellence in Research of Hereditary\nDisorders, King Abdulaziz University, Jeddah, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nabeel","family":"Bondagji","sequence":"additional","affiliation":[{"name":"Princess Al-Jawhara Al-Brahim Center of Excellence in Research of Hereditary\nDisorders, King Abdulaziz University, Jeddah, Saudi Arabia"},{"name":"Department of Obstetrics and Gynecology, Faculty of Medicine King Abdulaziz\nUniversity, Jeddah, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3061","published-online":{"date-parts":[[2020,9,7]]},"container-title":["Archives of Medical Science"],"original-title":[],"link":[{"URL":"https:\/\/www.archivesofmedicalscience.com\/pdf-122208-59526","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.archivesofmedicalscience.com\/pdf-122208-59526","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,29]],"date-time":"2021-10-29T14:18:13Z","timestamp":1635517093000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.archivesofmedicalscience.com\/Assessing-the-role-of-serum-prolactin-levels-and-coding-region-somatic-mutations,122208,0,2.html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,7]]},"references-count":0,"alternative-id":["122208"],"URL":"https:\/\/doi.org\/10.5114\/aoms.2020.98658","relation":{},"ISSN":["1734-1922","1896-9151"],"issn-type":[{"value":"1734-1922","type":"print"},{"value":"1896-9151","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,7]]}}}