{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T14:14:56Z","timestamp":1781532896727,"version":"3.54.5"},"reference-count":32,"publisher":"Termedia Sp. z.o.o.","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioTechnologia"],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>&lt;i&gt;Klebsiella pneumoniae&lt;\/i&gt; (KP) has re-emerged as a prominent etiological agent of pneumonia and systemic infection, with extended-spectrum \u03b2-lactamase (ESBL)-producing strains posing considerable therapeutic challenges. Moreover, vaccines may have limited efficacy because of the emergence of variant strains. The yidRv gene encodes a protein linked to excessive adherence and is conserved in over 99% of KP isolates. The present study aimed to explore the ability of a rec-YidRv protein to stimulate humoral and cellular immune response &lt;i&gt;in vitro&lt;\/i&gt; and &lt;i&gt;in vivo&lt;\/i&gt;.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material and methods<\/jats:title>\n                    <jats:p>The capability of the purified rec-YidRv protein to induce antibody synthesis was tested using a mice model. We also assessed whether rec-YidRv could successfully trigger the proliferation of splenocytes and the synthesis of their cytokines. Furthermore, the capacity of antigen to protect mice from ESBL-producing KP strain infection was investigated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>&lt;i&gt;In vitr&lt;\/i&gt;o and &lt;i&gt;in vivo&lt;\/i&gt; analyses revealed that mice immunized with rec-YidRv generated a strong antibody and memory response. Compared to non-stimulated controls, immunized mice showed an increase in measurable KP-directed immunoglobulin G (IgG) levels, with elevated IgG1 and IgG2b fractions; additionally, splenocyte proliferation expanded by 5-fold. Infection of mice with a lethal dose of KP following immunization markedly extended ani\u00admal survival and decreased the pathogen\u2019s ability to form ventricular biofilms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The rec-YidRv elicited robust antibody responses and a moderate expansion of cellular activity. Rec-YidRv is an effective protein-only platform for inhibiting infection by antibiotic-resistant KP. Future studies should incorporate refined adjuvants and improved administration methods to enhance protective responses.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.5114\/bta\/222175","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T13:46:20Z","timestamp":1781531180000},"source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of humoral and cellular immune responses induced by a novel YidRv subunit vaccine from hypervirulent Klebsiella pneumoniae"],"prefix":"10.5114","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6881-2676","authenticated-orcid":true,"given":"Tri Yudani M.","family":"Raras","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8315-4520","authenticated-orcid":false,"given":"Mauludy J.","family":"Ajrullah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2771-1609","authenticated-orcid":false,"given":"Lailia N.","family":"Rahma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5835-1653","authenticated-orcid":false,"given":"Ni Nyoman T.","family":"Puspaningsih","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4314-6961","authenticated-orcid":false,"given":"Rivo Y. 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