{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T21:33:51Z","timestamp":1774733631233,"version":"3.50.1"},"reference-count":37,"publisher":"Termedia Sp. z.o.o.","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioTechnologia"],"abstract":"<jats:p>Dipcadi montanum (Dalz.) Baker (Asparagaceae) is a rare scapigerous herb endemic to the Western Ghats, a\u00a0glo\u00adbal biodiversity hotspot running parallel to the western coast of India. This study reports the development of a\u00a0re\u00adproducible protocol for mass propagation of this underutilized geophyte using bulb scale and immature leaf base explants. Miniature bulblets were successfully induced from both types of explants after 4 and 8 weeks of cul\u00adture on full-strength semisolid MS basal medium fortified with 3% sucrose and varying levels of BAP (4.4\u201317.7\u00a0\u03bcM) and TDZ (4.5\u201318.1 \u03bcM). The addition of 2.7 \u03bcM NAA further enhanced the rate of microbulb induction. Rooting of the 8-week-old bulblets, obtained from both explants, was achieved with more than 90% effi\u00adciency on liquid as well as agar-gelled half-strength MS basal medium fortified with varying levels of IBA (2.46\u20139.84 \u03bcM) and NAA (2.68\u201310.74 \u03bcM), with or without 2.32 \u03bcM Kinetin. More than 95% of the rooted plants survived the initial accli\u00adma\u00adtization process under controlled ex-vitro conditions, and a survival rate of over 80% was recorded after 4\u00a0weeks of transfer to greenhouse conditions. After a brief dormancy, the regenerants resumed growth in the postmonsoon season and exhibited morphological resemblance to the donor plant. Comparative cytological ana\u00adlysis between the donor and 15 randomly selected regenerants revealed a stable somatic count of 2n = 20.<\/jats:p>","DOI":"10.5114\/bta.2024.139755","type":"journal-article","created":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T06:20:47Z","timestamp":1719382847000},"page":"159-167","source":"Crossref","is-referenced-by-count":1,"title":["Micropropagation of Dipcadi montanum (Dalz.) Baker\n(Asparagaceae): a rare scapigerous herb"],"prefix":"10.5114","volume":"105","author":[{"given":"Sautrik","family":"Basu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emadul","family":"Islam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debraj","family":"Chakraborty","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3061","published-online":{"date-parts":[[2024,6,25]]},"reference":[{"key":"ref-1361208","first-page":"1","volume-title":"Asphodelaceae","author":"Ali S","year":"2005","unstructured":"Ali S., Qaiser M. (2005) Asphodelaceae, Flora of Pakistan, Department of Botany, University of Karachi and Missouri Botanical Press, Missouri Botanical Gardens, St. Louis. 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(2020) In vitro propagation of Lilium longiflorum Bulbs using NAA and BAP plant growth regulator treatment. KnE Life Sciences."},{"issue":"1","key":"ref-1361216","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2307\/3001478","article-title":"Multiple range and multiple F tests","volume":"11","author":"Duncan D.B","year":"1955","unstructured":"Duncan D.B. (1955) Multiple range and multiple F tests. Biometrics. 11(1): 1-42.","journal-title":"Biometrics"},{"issue":"5","key":"ref-1361217","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1016\/S2222-1808(15)61056-8","article-title":"Flavonoid constituents of Dipcadi erythraeum Webb. & Berthel","volume":"6","author":"El-Shabrawy M.O","year":"2016","unstructured":"El-Shabrawy M.O., Marzouk M.M., Kawashty S.A., Hosni H.A., El Garf I.A., Sale N.A.M. (2016) Flavonoid constituents of Dipcadi erythraeum Webb. & Berthel. Asian Pacific J. Tropic. Dis. 6(5): 404\u2013405.","journal-title":"Asian Pacific J. Tropic. 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Shivaji University."},{"issue":"211","key":"ref-1361244","first-page":"1","article-title":"In vitro bulb formation of direct and indirect regeneration of Lilium orientalis cv.\u201cStarfighter\u201d plants","volume":"43","author":"Youssef N.M","year":"2019","unstructured":"Youssef N.M., Shaaban S.A., Ghareeb Z.F., Taha L.S. (2019) In vitro bulb formation of direct and indirect regeneration of Lilium orientalis cv.\u201cStarfighter\u201d plants. Bull. Nation. Res. Centre 43(211): 1\u20139.","journal-title":"Bull. Nation. Res. 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