{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T19:26:09Z","timestamp":1788895569337,"version":"build-2803163510"},"reference-count":27,"publisher":"Termedia Sp. z.o.o.","issue":"3","license":[{"start":{"date-parts":[[2016,10,25]],"date-time":"2016-10-25T00:00:00Z","timestamp":1477353600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"},{"start":{"date-parts":[[2016,10,25]],"date-time":"2016-10-25T00:00:00Z","timestamp":1477353600000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"},{"start":{"date-parts":[[2016,10,25]],"date-time":"2016-10-25T00:00:00Z","timestamp":1477353600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cent Eur J Immunol"],"abstract":"<b>Introduction: <\/b> It is well known that the presence of <i>Helicobacter pylori<\/i> in the stomach induces gastritis and causes an immune response. Exposure of gastric epithelial cell lines to this germ induces the secretion of interleukin-8 (IL-8), which is a potent PMN-activating chemotactic cytokine. Interleukin-8 is usually elevated in gastric biopsy samples of patients with <i>H. pylori<\/i>-associated gastritis and significantly increases in the supernatant of <i>in vitro<\/i> cultivated biopsy samples of gastric mucosa with active <i>H. pylori<\/i> gastritis. Interleukin-8 is an activating factor for leucocytes and other pro-inflammatory factors, free radicals, and proteolytic enzymes. That is why natural compounds potentially useful in therapy are still investigated \u2013 among them flavonoids. They reveal anti-oxidative and anti-inflammatory activities and significantly inhibit the gastric mucosa damage.<br \/>\n<b>The aim of the study<\/b> was the estimation of the anti-inflammatory effects of flavonoids on <i>H. pylori<\/i>-induced activation of human gastric adenocarcinoma cells (AGS). After infection of AGS cells by cag PAI (+) <i>H. pylori<\/i> <i>in vitro<\/i>, secretion of IL-8, effects of flavonoids on viability of AGS cells, and effects of flavonoids on increase of <i>H. pylori<\/i> were determined. Such flavones as chrysin, quercetin, kaemferide, flavanone, galangin, and kaempferol were examined.<br \/>\n<b>Results<\/b>: This study has shown an inhibitory effect of flavonoids on the release of IL-8 through infected AGS cells (except chrysin), and no toxic effects to AGS cells were observed. Galangin revealed antibacterial effects against <i>H. pylori<\/i>. Flavonoids limit the inflammatory process through the inhibition of IL-8 release in infected AGS cells with <i>H. pylori<\/i>. The strongest inhibitor of IL-8 was galangin.<\/jats:p>","DOI":"10.5114\/ceji.2016.63119","type":"journal-article","created":{"date-parts":[[2016,10,28]],"date-time":"2016-10-28T06:01:34Z","timestamp":1477634494000},"page":"229-235","source":"Crossref","is-referenced-by-count":22,"title":["The inhibitory effect of flavonoids on interleukin-8 release by human gastric adenocarcinoma (AGS) cells infected with cag PAI (+) <i>Helicobacter pylori<\/i>"],"prefix":"10.5114","volume":"41","author":[{"given":"Mariusz A.","family":"Skiba","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kornelia","family":"Szendzielorz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bogdan","family":"Mazur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wojciech","family":"Kr\u00f3l","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3061","published-online":{"date-parts":[[2016,10,25]]},"reference":[{"key":"ref-1555701","doi-asserted-by":"crossref","unstructured":"Zhang MJ, Meng FL, Ji XY, et al. 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