{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T09:55:07Z","timestamp":1767952507660,"version":"3.49.0"},"reference-count":24,"publisher":"Termedia Sp. z.o.o.","issue":"1","license":[{"start":{"date-parts":[[2024,4,19]],"date-time":"2024-04-19T00:00:00Z","timestamp":1713484800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"},{"start":{"date-parts":[[2024,4,19]],"date-time":"2024-04-19T00:00:00Z","timestamp":1713484800000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"},{"start":{"date-parts":[[2024,4,19]],"date-time":"2024-04-19T00:00:00Z","timestamp":1713484800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cent Eur J Immunol"],"abstract":"<jats:p>Fever is an adaptive host-defense response to infection and nowadays is rightly considered to be an expression of a healthy body and a well-functioning immune system. The condition is that it must be tightly regulated. Therefore, in individual cases, fever may be detrimental and should be treated. Specific excessive febrile reaction to pathogens which occurs after aseptic injuries is one among such cases. We previously found that among necrotic products, high mobility group box protein 1 (HMGB1) released from the site of aseptic injury affects immune effectors (cells) to mediate higher fever in response to further contact with bacterial lipopolysaccharide (LPS).&lt;br \/&gt;\nHere we observed that intraperitoneal (i.p.) pre-injection of recombinant HMGB1 (5 \u00b5g\/rat i.p.) provoked an increase in plasma levels of prostaglandin E2 (PGE2) in rats and augmented release of interleukin (IL)-1\u03b2 and IL-6 after LPS administration at a dose of 50 \u00b5g\/kg i.p. compared to rats pre-injected with saline or heat-denatured HMGB1. Furthermore, peripheral blood mononuclear cells (PBMCs) isolated from rats injected with HMGB1 were more sensitized to produce enhanced levels of IL-1\u03b2 and PGE2 when stimulated with LPS in vitro (1 \u00b5g\/ml\/10&lt;sup&gt;6&lt;\/sup&gt; cells for 4 h) compared to control animals injected with saline or heat-denatured HMGB1. We also noted a significant increase in activation of nuclear factor \u03baB (NF-\u03baB) in cells isolated from rats injected with HMGB1. Altogether, the obtained results suggest that HMGB1 participates in priming of immune cells to further contact with pathogens.<\/jats:p>","DOI":"10.5114\/ceji.2024.138600","type":"journal-article","created":{"date-parts":[[2024,4,29]],"date-time":"2024-04-29T09:30:28Z","timestamp":1714383028000},"page":"52-59","source":"Crossref","is-referenced-by-count":0,"title":["High mobility group box protein 1 sensitizes mononuclear cells to further contact with lipopolysaccharide"],"prefix":"10.5114","volume":"49","author":[{"given":"Jakub","family":"Piotrowski","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomasz","family":"J\u0119drzejewski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3061","published-online":{"date-parts":[[2024,4,19]]},"reference":[{"key":"ref-1535831","volume-title":"Institute of Medicine (US) Committee on Military Nutrition Research. Military Strategies for Sustainment of Nutrition and Immune Function in the Field","author":"Beisel WR","year":"1999","unstructured":"Beisel WR (1999): Overview of the immune system and other host defense mechanisms. In: Institute of Medicine (US) Committee on Military Nutrition Research. Military Strategies for Sustainment of Nutrition and Immune Function in the Field. National Academies Press, Washington (DC). Available from: https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK230976"},{"key":"ref-1535832","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.jtherbio.2019.05.028","article-title":"High mobility group box 1 protein released in the course of aseptic necrosis of tissues sensitizes rats to pyrogenic effects of lipopolysaccharide","volume":"84","author":"Piotrowski J","year":"2019","unstructured":"Piotrowski J, J\u0119drzejewski T, Pawlikowska M, et al. (2019): High mobility group box 1 protein released in the course of aseptic necrosis of tissues sensitizes rats to pyrogenic effects of lipopolysaccharide. J Therm Biol 84: 36-44.","journal-title":"J Therm Biol"},{"key":"ref-1535833","first-page":"101","article-title":"Emerging roles for HMGB1 protein in immunity, inflammation, and cancer","volume":"4","author":"Martinotti S","year":"2015","unstructured":"Martinotti S, Patrone M, Ranzato E (2015): Emerging roles for HMGB1 protein in immunity, inflammation, and cancer. Immuno Targets Ther 4: 101-109.","journal-title":"Immuno Targets Ther"},{"key":"ref-1535834","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1038\/nri1594","article-title":"High-mobility group box 1 protein (HMGB1): nuclear weapon in the immune arsenal","volume":"5","author":"Lotze MT","year":"2005","unstructured":"Lotze MT, Tracey KJ (2005): High-mobility group box 1 protein (HMGB1): nuclear weapon in the immune arsenal. Nat Rev Immunol 5: 331-342.","journal-title":"Nat Rev Immunol"},{"key":"ref-1535835","doi-asserted-by":"crossref","first-page":"33","DOI":"10.5114\/ceji.2019.84012","article-title":"Serum levels of the high-mobility group box 1 protein (HMGB1) in children with type 1 diabetes mellitus: case-control study","volume":"44","author":"Marjanac I","year":"2019","unstructured":"Marjanac I, Lovri\u0107 R, Barbi\u0107 J (2019): Serum levels of the high-mobility group box 1 protein (HMGB1) in children with type 1 diabetes mellitus: case-control study. Cent Eur J of Immunol 44: 33-37.","journal-title":"Cent Eur J of Immunol"},{"key":"ref-1535836","doi-asserted-by":"crossref","first-page":"6","DOI":"10.2119\/molmed.2015.00087","article-title":"High mobility group box protein 1 (HMGB1): the prototypical endogenous danger molecule","volume":"21","author":"Yang H","year":"2015","unstructured":"Yang H, Wang H, Chavan SS, Andersson U (2015): High mobility group box protein 1 (HMGB1): the prototypical endogenous danger molecule. Mol Med 21: 6-12.","journal-title":"Mol Med"},{"key":"ref-1535837","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.3349\/ymj.2014.55.5.1165","article-title":"The role of high mobility group box 1 in innate immunity","volume":"55","author":"Lee SA","year":"2014","unstructured":"Lee SA, Kwak MS, Kim S, Shin JS (2014): The role of high mobility group box 1 in innate immunity. Yonsei Med J 55: 1165-1176.","journal-title":"Yonsei Med J"},{"key":"ref-1535838","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1074\/jbc.270.43.25752","article-title":"The receptor for advanced glycation end products (RAGE) is a cellular binding site for amphoterin. Mediation of neurite outgrowth and co-expression of rage and amphoterin in the developing nervous system","volume":"270","author":"Hori O","year":"1995","unstructured":"Hori O, Brett J, Slattery T, et al. (1995): The receptor for advanced glycation end products (RAGE) is a cellular binding site for amphoterin. Mediation of neurite outgrowth and co-expression of rage and amphoterin in the developing nervous system. J Biol Chem 270: 52-61.","journal-title":"J Biol Chem"},{"key":"ref-1535839","doi-asserted-by":"crossref","first-page":"476","DOI":"10.2119\/2008-00034.Klune","article-title":"HMGB1: Endogenous danger signaling","volume":"14","author":"Klune JR","year":"2008","unstructured":"Klune JR, Dhupar R, Cardinal J, et al. (2008): HMGB1: Endogenous danger signaling. Mol Med 14: 476-484.","journal-title":"Mol Med"},{"key":"ref-1535840","doi-asserted-by":"crossref","first-page":"2648","DOI":"10.1152\/japplphysiol.01005.2001","article-title":"Invited review: Cytokine regulation of fever: studies using gene knockout mice","volume":"92","author":"Leon LR","year":"2002","unstructured":"Leon LR (2002): Invited review: Cytokine regulation of fever: studies using gene knockout mice. 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Imm Lett 177: 1-5.","journal-title":"Imm Lett"},{"key":"ref-1535842","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.1002\/eji.200424916","article-title":"Mini-review: The nuclear protein HMGB1 as a proinflammatory mediator","volume":"34","author":"Erlandsson HH","year":"2004","unstructured":"Erlandsson HH, Andersson U (2004): Mini-review: The nuclear protein HMGB1 as a proinflammatory mediator. Eur J Immunol 34: 1503-1512.","journal-title":"Eur J Immunol"},{"key":"ref-1535843","doi-asserted-by":"crossref","first-page":"e50668","DOI":"10.1371\/journal.pone.0050668","article-title":"High-mobility group box-1 induces proinflammatory cytokines production of Kupffer cells through TLRs-dependent signaling pathway after burn injury","volume":"7","author":"Chen XL","year":"2012","unstructured":"Chen XL, Sun L, Guo F, et al. (2012): High-mobility group box-1 induces proinflammatory cytokines production of Kupffer cells through TLRs-dependent signaling pathway after burn injury. 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(2013): IL-1\u03b2\/HMGB1 complexes promote The PGE2 biosynthesis pathway in synovial fibroblasts. Scand J Immunol 77: 350-360.","journal-title":"Scand J Immunol"},{"key":"ref-1535846","first-page":"R1179","article-title":"Fever and thermogenesis in response to bacterial endotoxin involve macrophage-dependent mechanisms in rats","volume":"265","author":"Derijk RH","year":"1993","unstructured":"Derijk RH, Strijbos PJ, van Rooijen N, et al. (1993): Fever and thermogenesis in response to bacterial endotoxin involve macrophage-dependent mechanisms in rats. Am J Physiol 265: R1179-1183.","journal-title":"Am J Physiol"},{"key":"ref-1535847","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1189\/jlb.0908548","article-title":"The alarmin HMGB1 acts in synergy with endogenous and exogenous danger signals to promote inflammation","volume":"86","author":"Hreggvidsdottir HS","year":"2009","unstructured":"Hreggvidsdottir HS, Ostberg T, W\u00e4h\u00e4maa H, et al. (2009): The alarmin HMGB1 acts in synergy with endogenous and exogenous danger signals to promote inflammation. J Leukoc Biol 86: 655-662.","journal-title":"J Leukoc Biol"},{"key":"ref-1535848","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1186\/ar3450","article-title":"High mobility group box protein 1 in complex with lipopolysaccharide or IL-1 promotes an increased inflammatory phenotype in synovial fibroblasts","volume":"13","author":"W\u00e4h\u00e4maa H","year":"2011","unstructured":"W\u00e4h\u00e4maa H, Schierbeck H, Hreggvidsdottir HS, et al. (2011): High mobility group box protein 1 in complex with lipopolysaccharide or IL-1 promotes an increased inflammatory phenotype in synovial fibroblasts. 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