{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T17:12:08Z","timestamp":1788887528191,"version":"build-2803163510"},"reference-count":0,"publisher":"Termedia Sp. z.o.o.","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pol J Pathol"],"abstract":"<jats:p>The purpose of this paper was to explore the immunohistochemistry (IHC) results for a cocktail of minichromosome maintenance protein 2 (MCM2) and topoisomerase II\uf061 (TOP2A), p16&lt;sup&gt;INK4a&lt;\/sup&gt; and Ki-67 as biomarkers for the diagnosis of cervical intraepithelial neoplasia (CIN), improving the routine interpretation of cervical histopathology. 133 cases of CIN were collected from the archival data. All routine hematoxylin and eosin (HE)-stained slides of the subjects were re-examined independently by three senior pathologists, to provide a \u201cconsensus diagnosis\u201d. Immunohistochemistry for the three biomarkers was performed, and the results were reviewed independently of the corresponding archival diagnosis to make a \u201cdiagnosis assisted by IHC\u201d by the original pathological practitioners. The diagnosis accordance rate of the archival original diagnosis with the \u201cconsensus diagnosis\u201d and the \u201cdiagnosis assisted by IHC\u201d with the \u201cconsensus diagnosis\u201d were verified by Fisher\u2019s exact test. The results showed that raw agreement between the original HE diagnosis and the \u201cconsensus diagnosis\u201d was 88.55%, and raw agreement between the \u201cdiagnosis assisted by IHC\u201d and the \u201cconsensus diagnosis\u201d was 95.78%. The latter was significantly higher than the former (Fisher\u2019s exact test, p = 0.023). In conclusion, the three biomarkers had a high degree of sensitivity and specificity, and appear to be a useful and reliable diagnostic adjunct to improve the routine diagnosis, and reduce inter-observer variability in cervical biopsy specimens.<\/jats:p>","DOI":"10.5114\/pjp.2013.34599","type":"journal-article","created":{"date-parts":[[2013,7,8]],"date-time":"2013-07-08T07:07:00Z","timestamp":1373267220000},"page":"21-27","source":"Crossref","is-referenced-by-count":9,"title":["A cocktail of MCM2 and TOP2A, p16&lt;sup&gt;INK4a&lt;\/sup&gt; and Ki-67 as biomarkers for the improved diagnosis of cervical intraepithelial lesion"],"prefix":"10.5114","volume":"64","author":[{"given":"Qi-chang","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-bing","family":"Liou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-juan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-hua","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3061","published-online":{"date-parts":[[2013,4,25]]},"container-title":["Polish Journal of Pathology"],"original-title":[],"link":[{"URL":"https:\/\/www.polishjournalofpathology.com\/pdf-54543-137240","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.polishjournalofpathology.com\/pdf-54543-137240","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T09:39:33Z","timestamp":1782207573000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.polishjournalofpathology.com\/A-cocktail-of-MCM2-and-TOP2A-p16-INK4a-and-Ki-67-as-biomarkers-for-the-improved-diagnosis,54543,0,2.html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,4,25]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013]]}},"alternative-id":["54543"],"URL":"https:\/\/doi.org\/10.5114\/pjp.2013.34599","relation":{},"ISSN":["1233-9687","2084-9869"],"issn-type":[{"value":"1233-9687","type":"print"},{"value":"2084-9869","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,4,25]]}}}