{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T10:51:34Z","timestamp":1782211894291,"version":"3.54.5"},"reference-count":48,"publisher":"Termedia Sp. z.o.o.","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pol J Pathol"],"abstract":"<jats:p>The purpose of\u00a0the\u00a0study was to investigate HPV16 infection in laryngeal cancer patients treated with surgery and adjuvant radiotherapy as well as to analyze treatment results in relation to HPV16 infection and selected clinical, histopathological, and radiotherapy parameters.&lt;br \/&gt;\nA retrospective analysis was performed in a\u00a0group of\u00a060 patients with squamous cell carcinoma of\u00a0the\u00a0larynx treated surgically and qualified for adjuvant radiotherapy at the\u00a0Oncology Center in Cracow between 1995 and 2001. The\u00a0studied group consisted of\u00a057 men (95%) and 3 women (5%) of\u00a0mean age of\u00a056 years. In &lt;br \/&gt;\n13 patients (22%) underweight was noted. In the\u00a0analyzed material, locally advanced laryngeal cancer prevailed (pT3-pT4) \u2013 52 cases (87%), with the\u00a0involvement of\u00a0cervical lymph nodes (pN+) \u2013 32 cases (53%). Histopathological examination revealed that microscopic radicality was not obtained in 18 patients (30%). Human papillomavirus 16 infection status as well as infection type (integrated, episomal, or mixed) were assessed in each patient by means of\u00a0quantitative polymerase chain reaction (qPCR) using real-time detection.&lt;br \/&gt;\nThe 5-year OS, DFS, and LC rates were 45%, 61%, and 69%, respectively. Multivariate analysis revealed that local relapse risk and local failure risk were statistically significantly influenced by underweight and positive surgical margin. Underweight had also a\u00a0statistically significant impact on death risk. The\u00a0HPV16 infection was noticed in 4 cancers (6.8%). In all cases it was the\u00a0same episomal type. &lt;br \/&gt;\nOn the\u00a0basis of\u00a0our observations it can be assumed that HPV infection does not play an\u00a0important role in etiology of\u00a0laryngeal cancer. Although, further study is needed in larger patient populations; optimal methodology for detecting HPV infection should also be determined. Positive surgical margin has a\u00a0significant effect on worse treatment outcomes. Underweight before radiotherapy diminishes the\u00a0probability of\u00a0treatment success and survival of\u00a0laryngeal cancer patients.<\/jats:p>","DOI":"10.5114\/pjp.2021.104659","type":"journal-article","created":{"date-parts":[[2021,7,4]],"date-time":"2021-07-04T15:12:10Z","timestamp":1625411530000},"page":"64-74","source":"Crossref","is-referenced-by-count":1,"title":["Assessment of HPV16 infection in patients with laryngeal cancer"],"prefix":"10.5114","volume":"72","author":[{"given":"Anna","family":"Mucha-Ma\u0142ecka","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beata","family":"Biesaga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Janecka-Wid\u0142a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcin","family":"Przewo\u017anik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krzysztof","family":"Ma\u0142ecki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3061","published-online":{"date-parts":[[2021,5,31]]},"reference":[{"key":"ref-1695208","unstructured":"Didkowska J, Wojciechowska U, Olasek P. 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