{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T22:58:54Z","timestamp":1782255534936,"version":"3.54.5"},"reference-count":28,"publisher":"Termedia Sp. z.o.o.","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pol J Pathol"],"abstract":"<jats:p>Clinical autopsies are performed to reveal the process of the disease that caused patient death and validate the diagnosis and treatment decisions. In pediatric clinical autopsy, the feedback provided to bereaved families has a\u00a0considerable social impact; however, pediatric diseases are diverse, which makes it difficult to elucidate them. Therefore, it is necessary to employ molecular biology techniques in addition to conventional methods. Formalin-fixed, paraffin-embedded (FFPE) tissues are routinely prepared. However, clinical autopsy FFPE tissue processing is not standardized, and it is unclear whether DNA from such tissues can be used for comprehensive genomic analysis. &lt;br \/&gt;\nIn this study, we evaluated the DNA quality of FFPE tissues from 15 recent autopsy cases at a\u00a0single-center children\u2019s hospital using quantitative polymerase chain reaction [PCR (Q129\/Q41)] and nanoelectrophoresis (DNA integrity number (DIN)). &lt;br \/&gt;\nGood quality DNA was obtained from every organ type excluding bone marrow within 6 days of formalin fixation. Prolonged proteinase K digestion (48 h &gt; 24 h &gt; 1 h) and thicker tissue sections (10 \u00b5m &gt; 1 \u00b5m) improved Q129\/Q41; however, 24 h fixed FFPE tissues showed better DNA quality. &lt;br \/&gt;\nWe propose an optimal and feasible workflow for storing short-term fixed FFPE tissues as DNA-preserved FFPE tissues for future comprehensive genomic searches.<\/jats:p>","DOI":"10.5114\/pjp.2022.124492","type":"journal-article","created":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T02:37:43Z","timestamp":1675046263000},"page":"255-263","source":"Crossref","is-referenced-by-count":3,"title":["Optimal DNA quality preservation process for comprehensive genomic testing of pediatric clinical autopsy formalin-fixed, paraffin-embedded tissues"],"prefix":"10.5114","volume":"73","author":[{"given":"Noriko","family":"Watanabe","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohiro","family":"Umezu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masashi","family":"Kyushiki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kayoko","family":"Ichimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsuko","family":"Nakazawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiko","family":"Kuroda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3061","published-online":{"date-parts":[[2023,1,10]]},"reference":[{"key":"ref-1693082","doi-asserted-by":"crossref","unstructured":"Kotabagi RB, Charati SC, Jayachandar D. 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J Surg Res 2017; 220: 125-132.","DOI":"10.1016\/j.jss.2017.06.077"},{"key":"ref-1693094","doi-asserted-by":"crossref","unstructured":"Kanai Y, Nishihara H, Miyagi Y, et al. The Japanese Society of Pathology guidelines on the handling of pathological tissue samples for genomic research: standard operating procedures based on empirical analyses. Pathol Int 2018; 68: 63-90.","DOI":"10.1111\/pin.12631"},{"key":"ref-1693095","doi-asserted-by":"crossref","unstructured":"Hatanaka Y, Kuwata T, Morii E, et al. The Japanese Society of Pathology Practical Guidelines on the handling of pathological tissue samples for cancer genomic medicine. Pathol Int 2021; 71: 725-740.","DOI":"10.1111\/pin.13170"},{"key":"ref-1693096","unstructured":"The Japanese Society of Pathology. Guidelines for the handling of pathological tissue samples for genomic medicine. 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Effect of different proteinase K digest protocols and deparaffinization methods on yield and integrity of DNA extracted from formalin-fixed, paraffin- embedded tissue. 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